8 research outputs found
Protein-Tyrosine Kinase Activity Profiling in Knock Down Zebrafish Embryos
BACKGROUND: Protein-tyrosine kinases (PTKs) regulate virtually all biological processes. PTKs phosphorylate substrates in a sequence-specific manner and relatively short peptide sequences determine selectivity. Here, we developed new technology to determine PTK activity profiles using peptide arrays. The zebrafish is an excellent model system to investigate signaling in the whole organism, given its wealth of genetic tools, including morpholino-mediated knock down technology. We used zebrafish embryo lysates to determine PTK activity profiles, thus providing the unique opportunity to directly compare the effect of protein knock downs on PTK activity profiles on the one hand and phenotypic changes on the other. METHODOLOGY: We used multiplex arrays of 144 distinct peptides, spotted on a porous substrate, allowing the sample to be pumped up and down, optimizing reaction kinetics. Kinase reactions were performed using complex zebrafish embryo lysates or purified kinases. Peptide phosphorylation was detected by fluorescent anti-phosphotyrosine antibody binding and the porous chips allowed semi-continuous recording of the signal. We used morpholinos to knock down protein expression in the zebrafish embryos and subsequently, we determined the effects on the PTK activity profiles. RESULTS AND CONCLUSION: Reproducible PTK activity profiles were derived from one-day-old zebrafiish embryos. Morpholino-mediated knock downs of the Src family kinases, Fyn and Yes, induced characteristic phenotypes and distinct changes in the PTK activity profiles. Interestingly, the peptide substrates that were less phosphorylated upon Fyn and Yes knock down were preferential substrates of purified Fyn and Yes. Previously, we demonstrated that Wnt11 knock down phenocopied Fyn/Yes knock down. Interestingly, Wnt11 knock down induced similar changes in the PTK activity profile as Fyn/Yes knock down. The control Nacre/Mitfa knock down did not affect the PTK activity profile significantly. Our results indicate that the novel peptide chip technology can be used to unravel kinase signaling pathways in vivo
The Cis-regulatory Logic of the Mammalian Photoreceptor Transcriptional Network
The photoreceptor cells of the retina are subject to a greater number of genetic diseases than any other cell type in the human body. The majority of more than 120 cloned human blindness genes are highly expressed in photoreceptors. In order to establish an integrative framework in which to understand these diseases, we have undertaken an experimental and computational analysis of the network controlled by the mammalian photoreceptor transcription factors, Crx, Nrl, and Nr2e3. Using microarray and in situ hybridization datasets we have produced a model of this network which contains over 600 genes, including numerous retinal disease loci as well as previously uncharacterized photoreceptor transcription factors. To elucidate the connectivity of this network, we devised a computational algorithm to identify the photoreceptor-specific cis-regulatory elements (CREs) mediating the interactions between these transcription factors and their target genes. In vivo validation of our computational predictions resulted in the discovery of 19 novel photoreceptor-specific CREs near retinal disease genes. Examination of these CREs permitted the definition of a simple cis-regulatory grammar rule associated with high-level expression. To test the generality of this rule, we used an expanded form of it as a selection filter to evolve photoreceptor CREs from random DNA sequences in silico. When fused to fluorescent reporters, these evolved CREs drove strong, photoreceptor-specific expression in vivo. This study represents the first systematic identification and in vivo validation of CREs in a mammalian neuronal cell type and lays the groundwork for a systems biology of photoreceptor transcriptional regulation
Genomic analyses in Cornelia de Lange Syndrome and related diagnoses: Novel candidate genes, <scp>genotype–phenotype</scp> correlations and common mechanisms
Cornelia de Lange Syndrome (CdLS) is a rare, dominantly inherited multisystem developmental disorder characterized by highly variable manifestations of growth and developmental delays, upper limb involvement, hypertrichosis, cardiac, gastrointestinal, craniofacial, and other systemic features. Pathogenic variants in genes encoding cohesin complex structural subunits and regulatory proteins (NIPBL, SMC1A, SMC3, HDAC8, and RAD21) are the major pathogenic contributors to CdLS. Heterozygous or hemizygous variants in the genes encoding these five proteins have been found to be contributory to CdLS, with variants in NIPBL accounting for the majority (>60%) of cases, and the only gene identified to date that results in the severe or classic form of CdLS when mutated. Pathogenic variants in cohesin genes other than NIPBL tend to result in a less severe phenotype. Causative variants in additional genes, such as ANKRD11, EP300, AFF4, TAF1, and BRD4, can cause a CdLS‐like phenotype. The common role that these genes, and others, play as critical regulators of developmental transcriptional control has led to the conditions they cause being referred to as disorders of transcriptional regulation (or “DTRs”). Here, we report the results of a comprehensive molecular analysis in a cohort of 716 probands with typical and atypical CdLS in order to delineate the genetic contribution of causative variants in cohesin complex genes as well as novel candidate genes, genotype–phenotype correlations, and the utility of genome sequencing in understanding the mutational landscape in this population
Cornelia de Lange syndrome in diverse populations
Cornelia de Lange syndrome (CdLS) is a dominant multisystemic malformation syndrome due to mutations in five genes—NIPBL, SMC1A, HDAC8, SMC3, and RAD21. The characteristic facial dysmorphisms include microcephaly, arched eyebrows, synophrys, short nose with depressed bridge and anteverted nares, long philtrum, thin lips, micrognathia, and hypertrichosis. Most affected individuals have intellectual disability, growth deficiency, and upper limb anomalies. This study looked at individuals from diverse populations with both clinical and molecularly confirmed diagnoses of CdLS by facial analysis technology. Clinical data and images from 246 individuals with CdLS were obtained from 15 countries. This cohort included 49% female patients and ages ranged from infancy to 37 years. Individuals were grouped into ancestry categories of African descent, Asian, Latin American, Middle Eastern, and Caucasian. Across these populations, 14 features showed a statistically significant difference. The most common facial features found in all ancestry groups included synophrys, short nose with anteverted nares, and a long philtrum with thin vermillion of the upper lip. Using facial analysis technology we compared 246 individuals with CdLS to 246 gender/age matched controls and found that sensitivity was equal or greater than 95% for all groups. Specificity was equal or greater than 91%. In conclusion, we present consistent clinical findings from global populations with CdLS while demonstrating how facial analysis technology can be a tool to support accurate diagnoses in the clinical setting. This work, along with prior studies in this arena, will assist in earlier detection, recognition, and treatment of CdLS worldwide.Supplementary Table 1 Participants with photographs in Figures 2-5 from 10 countries.
Supplementary Table 2. Geometric and texture feature comparison of Global (combined African descent, Asian, Latin American, Caucasian) CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 3. Geometric and texture feature comparison of African descent CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 4. Geometric and texture feature comparison of Asian CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 5. Geometric and texture feature comparison of Latin American CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 6. Geometric and texture feature comparison of Caucasian CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Figure 1. Global: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 2. African: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 3. Asian: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 4. Latin American: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 5. Caucasian: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selectedPK and MM are supported by the Division of Intramural Research at the National Human Genome Research, NIH. Partial funding of this project was from a philanthropic gift from the Government of Abu Dhabi to the Children's National Health System. VS is supported by the Chulalongkorn Academic Advancement Into Its 2nd Century Project and the Thailand Research Fund. We would also like to acknowledge other clinicians who supported this work—MZ, JP, and GC. We would like to acknowledge that IDK, LD, MK, and SR are supported by the CdLS Center Endowed Funds at The Children's Hospital of Philadelphia and PO1 HD052860 from the NICHD. ES is supported by a fellowship from PKS Italia and PKSKids USA. LD was also supported by a postdoctoral training grant (T32 GM008638) from the NIGMS.http://wileyonlinelibrary.com/journal/ajmga2020-02-01hj2019Genetic
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Cornelia de Lange syndrome in diverse populations.
Cornelia de Lange syndrome (CdLS) is a dominant multisystemic malformation syndrome due to mutations in five genes-NIPBL, SMC1A, HDAC8, SMC3, and RAD21. The characteristic facial dysmorphisms include microcephaly, arched eyebrows, synophrys, short nose with depressed bridge and anteverted nares, long philtrum, thin lips, micrognathia, and hypertrichosis. Most affected individuals have intellectual disability, growth deficiency, and upper limb anomalies. This study looked at individuals from diverse populations with both clinical and molecularly confirmed diagnoses of CdLS by facial analysis technology. Clinical data and images from 246 individuals with CdLS were obtained from 15 countries. This cohort included 49% female patients and ages ranged from infancy to 37 years. Individuals were grouped into ancestry categories of African descent, Asian, Latin American, Middle Eastern, and Caucasian. Across these populations, 14 features showed a statistically significant difference. The most common facial features found in all ancestry groups included synophrys, short nose with anteverted nares, and a long philtrum with thin vermillion of the upper lip. Using facial analysis technology we compared 246 individuals with CdLS to 246 gender/age matched controls and found that sensitivity was equal or greater than 95% for all groups. Specificity was equal or greater than 91%. In conclusion, we present consistent clinical findings from global populations with CdLS while demonstrating how facial analysis technology can be a tool to support accurate diagnoses in the clinical setting. This work, along with prior studies in this arena, will assist in earlier detection, recognition, and treatment of CdLS worldwide
Cornelia de Lange Syndrome in Diverse Populations.
Cornelia de Lange syndrome (CdLS) is a dominant multisystemic malformation syndrome due to mutations in five genes—NIPBL, SMC1A, HDAC8, SMC3, and RAD21. The characteristic facial dysmorphisms include microcephaly, arched eyebrows, synophrys, short nose with depressed bridge and anteverted nares, long philtrum, thin lips, micrognathia, and hypertrichosis. Most affected individuals have intellectual disability, growth deficiency, and upper limb anomalies. This study looked at individuals from diverse populations with both clinical and molecularly confirmed diagnoses of CdLS by facial analysis technology. Clinical data and images from 246 individuals with CdLS were obtained from 15 countries. This cohort included 49% female patients and ages ranged from infancy to 37 years. Individuals were grouped into ancestry categories of African descent, Asian, Latin American, Middle Eastern, and Caucasian. Across these populations, 14 features showed a statistically significant difference. The most common facial features found in all ancestry groups included synophrys, short nose with anteverted nares, and a long philtrum with thin vermillion of the upper lip. Using facial analysis technology we compared 246 individuals with CdLS to 246 gender/age matched controls and found that sensitivity was equal or greater than 95% for all groups. Specificity was equal or greater than 91%. In conclusion, we present consistent clinical findings from global populations with CdLS while demonstrating how facial analysis technology can be a tool to support accurate diagnoses in the clinical setting. This work, along with prior studies in this arena, will assist in earlier detection, recognition, and treatment of CdLS worldwide.Supplementary Table 1 Participants with photographs in Figures 2-5 from 10 countries.
Supplementary Table 2. Geometric and texture feature comparison of Global (combined African descent, Asian, Latin American, Caucasian) CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 3. Geometric and texture feature comparison of African descent CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 4. Geometric and texture feature comparison of Asian CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 5. Geometric and texture feature comparison of Latin American CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Table 6. Geometric and texture feature comparison of Caucasian CdLS individuals with normal controls using digital facial analysis technology. The ranges of the geometric linear features were normalized by the ear‐to‐ear distance. Geometric angle features are presented in degrees. Texture features were computed at three scales (r1, r2, and r3). Features are presented in order of their relevance for the diagnosis of CdLS.
Supplementary Figure 1. Global: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 2. African: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 3. Asian: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 4. Latin American: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selected.
Supplementary Figure 5. Caucasian: Graph of area under the ROC curve (AUC), accuracy, sensitivity, and specificity versus the number of features selectedPK and MM are supported by the Division of Intramural Research at the National Human Genome Research, NIH. Partial funding of this project was from a philanthropic gift from the Government of Abu Dhabi to the Children's National Health System. VS is supported by the Chulalongkorn Academic Advancement Into Its 2nd Century Project and the Thailand Research Fund. We would also like to acknowledge other clinicians who supported this work—MZ, JP, and GC. We would like to acknowledge that IDK, LD, MK, and SR are supported by the CdLS Center Endowed Funds at The Children's Hospital of Philadelphia and PO1 HD052860 from the NICHD. ES is supported by a fellowship from PKS Italia and PKSKids USA. LD was also supported by a postdoctoral training grant (T32 GM008638) from the NIGMS.http://wileyonlinelibrary.com/journal/ajmga2020-02-01hj2019Genetic
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HNRNPC haploinsufficiency affects alternative splicing of intellectual disability-associated genes and causes a neurodevelopmental disorder
Heterogeneous nuclear ribonucleoprotein C (HNRNPC) is an essential, ubiquitously abundant protein involved in mRNA processing. Genetic variants in other members of the HNRNP family have been associated with neurodevelopmental disorders. Here, we describe 13 individuals with global developmental delay, intellectual disability, behavioral abnormalities, and subtle facial dysmorphology with heterozygous HNRNPC germline variants. Five of them bear an identical in-frame deletion of nine amino acids in the extreme C terminus. To study the effect of this recurrent variant as well as HNRNPC haploinsufficiency, we used induced pluripotent stem cells (iPSCs) and fibroblasts obtained from affected individuals. While protein localization and oligomerization were unaffected by the recurrent C-terminal deletion variant, total HNRNPC levels were decreased. Previously, reduced HNRNPC levels have been associated with changes in alternative splicing. Therefore, we performed a meta-analysis on published RNA-seq datasets of three different cell lines to identify a ubiquitous HNRNPC-dependent signature of alternative spliced exons. The identified signature was not only confirmed in fibroblasts obtained from an affected individual but also showed a significant enrichment for genes associated with intellectual disability. Hence, we assessed the effect of decreased and increased levels of HNRNPC on neuronal arborization and neuronal migration and found that either condition affects neuronal function. Taken together, our data indicate that HNRNPC haploinsufficiency affects alternative splicing of multiple intellectual disability-associated genes and that the developing brain is sensitive to aberrant levels of HNRNPC. Hence, our data strongly support the inclusion of HNRNPC to the family of HNRNP-related neurodevelopmental disorders.
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We identified genetic variants of HNRNPC in 13 individuals with intellectual disability and global developmental delay. Through a meta-analysis of multiple cell types, we found that loss of HNRNPC affects alternative splicing, in particular of intellectual disability-associated genes. In vivo assays confirmed that neurodevelopment was affected by aberrant HNRNPC levels