An algal enzyme required for biosynthesis of the most abundant marine carotenoids.

Fucoxanthin and its derivatives are the main light-harvesting pigments in the photosynthetic apparatus of many chromalveolate algae and represent the most abundant carotenoids in the world's oceans, thus being major facilitators of marine primary production. A central step in fucoxanthin biosynthesis that has been elusive so far is the conversion of violaxanthin to neoxanthin. Here, we show that in chromalveolates, this reaction is catalyzed by violaxanthin de-epoxidase-like (VDL) proteins and that VDL is also involved in the formation of other light-harvesting carotenoids such as peridinin or vaucheriaxanthin. VDL is closely related to the photoprotective enzyme violaxanthin de-epoxidase that operates in plants and most algae, revealing that in major phyla of marine algae, an ancient gene duplication triggered the evolution of carotenoid functions beyond photoprotection toward light harvesting

High light and temperature reduce photosynthetic efficiency through different mechanisms in the C4 model Setaria viridis.

Funder: start-up funding from Donald Danforth Plant Science CenterC4 plants frequently experience high light and high temperature conditions in the field, which reduce growth and yield. However, the mechanisms underlying these stress responses in C4 plants have been under-explored, especially the coordination between mesophyll (M) and bundle sheath (BS) cells. We investigated how the C4 model plant Setaria viridis responded to a four-hour high light or high temperature treatment at photosynthetic, transcriptomic, and ultrastructural levels. Although we observed a comparable reduction of photosynthetic efficiency in high light or high temperature treated leaves, detailed analysis of multi-level responses revealed important differences in key pathways and M/BS specificity responding to high light and high temperature. We provide a systematic analysis of high light and high temperature responses in S. viridis, reveal different acclimation strategies to these two stresses in C4 plants, discover unique light/temperature responses in C4 plants in comparison to C3 plants, and identify potential targets to improve abiotic stress tolerance in C4 crops

The spread-F Experiment (SpreadFEx): Program overview and first results

We performed an extensive experimental campaign (the spread F Experiment, or SpreadFEx) from September to November 2005 to attempt to define the role of neutral atmosphere dynamics, specifically wave motions propagating upward from the lower atmosphere, in seeding equatorial spread F and plasma bubbles extending to higher altitudes. Campaign measurements focused on the Brazilian sector and included ground-based optical, radar, digisonde, and GPS measurements at a number of fixed and temporary sites. Related data on convection and plasma bubble structures were also collected by GOES 12 and the GUVI instrument aboard the TIMED satellite. Initial results of our analyses of SpreadFEx and related data indicate 1) extensive gravity wave (GW) activity apparently linked to deep convection predominantly to the west of our measurement sites, 2) the presence of small-scale GWactivity confined to lower altitudes, 3) larger-scaleGWactivity apparently penetrating to much higher altitudes suggested by electron density and TEC fluctuations in the E and F regions, 4) substantial GW amplitudes implied by digisonde electron densities, and 5) apparent direct links of these perturbations in the lower F region to spread F and plasma bubbles extending to much higher altitudes. Related efforts with correlative data are defining 6) the occurrence and locations of deep convection, 7) the spatial and temporal evolutions of plasma bubbles, the 8) 2D (height-resolved) structures of plasma bubbles, and 9) the expected propagation of GWs and tides from the lower atmosphere into the thermosphere and ionosphere

Overview and Summary of the Spread F Experiment (SpreadFex)

We provide here an overview of, and a summary of results arising from, an extensive experimental campaign (the Spread F Experiment, or SpreadFEx) performed from September to November 2005, with primary measurements in Brazil. The motivation was to define the potential role of neutral atmosphere dynamics, specifically gravity wave motions propagating upward from the lower atmosphere, in seeding Rayleigh-Taylor instability (RTI) and plasma bubbles extending to higher altitudes. Campaign measurements focused on the Brazilian sector and included ground-based optical, radar, digisonde, and GPS measurements at a number of fixed and temporary sites. Related data on convection and plasma bubble structures were also collected by GOES 12, and the GUVI instrument aboard the TIMED satellite

Writing Toward Readers\u27 Better Health: A Case Study Examining the Development of Online Health Information

Each year, more people search the Internet for health information. Through a case study conducted at a prominent health information company, I will show that technical communicators are well suited to contribute to the development of online health information. Like other technical communicators, online health information developers must make rhetorical choices based on audience needs, function within specific social contexts, and work through challenges of writing, editing, and project management

Propagation of Plasma Bubbles Observed in Brazil from GPS and Airglow Data

Equatorial spread-F is a common occurrence in the equatorial ionosphere that is associated with large variations in plasma density that often cause scintillation and interference in communication signals. These events are known to result from Rayleigh–Taylor instability, but the day-to-day variability of their occurrence is not well understood. The triggering mechanism of plasma depletions is still a matter of debate, but may be linked to gravity waves that under favorable conditions propagate to the middle atmosphere. Understanding the triggering of ESF was the focus of the SpreadFEx campaign near Brasilia, Brazil in 2005. The campaign provided co-located airglow and GPS observations to study the onset of plasma depletions and their evolution as they traversed the region. Comparisons between the 630.0 nm airglow data and GPS data demonstrate the ability of the compact dual frequency GPS array to detect the plasma bubbles and retrieve reliable propagation characteristics of the depletions. In this case study, a plasma depletion was detected and moved over the array at velocities of 85–110 m/s, slowing as it moved towards the east. Correlation of consecutive airglow images gives consistent estimates of the eastward drift over the same time period. Mapping the airglow data to the GPS line-of-sight geometry allows direct comparison and reveals a resolvable westward tilt of the plasma depletion that may be due to vertical shear. The uniqueness of this study is the ability to resolve locally the characteristics of the plasma depletion without relying on assumptions about the mapping of the depletion along magnetic field lines to large latitudinal distances. It presents new information for understanding ESF development and the development of depletions strong enough to produce scintillation

Properties and Propagation of Plasma Bubbles Observed Over Brazil During the SpreadFEx Campaign 2005

Plasma irregularities in equatorial Spread F region during the night time can significantly affect low latitude communications and navigation systems. The morphology, structure, and dynamics of equatorial plasma irregularities (or plasma bubbles) have been extensively studied by different authors for several decades by using different techniques (Woodman and LaHoz, 1976, Taylor et al., 1997, Kudeki et al., 1999, Sobral et al., 2002). The variability in the occurrence of plasma bubbles and their intensity constituent an important component of the space weather conditions of the equatorial ionosphere. The plasma depletion zonal velocity is a very important parameter for the understanding and modeling of the electrodynamics of the equatorial ionosphere and for the predictions of ionospheric scintillations

Propagation of plasma bubbles observed in Brazil from GPS and airglow data

Equatorial spread-F is a common occurrence in the equatorial ionosphere that is associated with large variations in plasma density that often cause scintillation and interference in communication signals. These events are known to result from Rayleigh–Taylor instability, but the day-to-day variability of their occurrence is not well understood. The triggering mechanism of plasma depletions is still a matter of debate, but may be linked to gravity waves that under favorable conditions propagate to the middle atmosphere. Understanding the triggering of ESF was the focus of the SpreadFEx campaign near Brasilia, Brazil in 2005. The campaign provided co-located airglow and GPS observations to study the onset of plasma depletions and their evolution as they traversed the region. Comparisons between the 630.0 nm airglow data and GPS data demonstrate the ability of the compact dual frequency GPS array to detect the plasma bubbles and retrieve reliable propagation characteristics of the depletions. In this case study, a plasma depletion was detected and moved over the array at velocities of 85–110 m/s, slowing as it moved towards the east. Correlation of consecutive airglow images gives consistent estimates of the eastward drift over the same time period. Mapping the airglow data to the GPS line-of-sight geometry allows direct comparison and reveals a resolvable westward tilt of the plasma depletion that may be due to vertical shear. The uniqueness of this study is the ability to resolve locally the characteristics of the plasma depletion without relying on assumptions about the mapping of the depletion along magnetic field lines to large latitudinal distances. It presents new information for understanding ESF development and the development of depletions strong enough to produce scintillation