Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae

This article is available open access through the publisher’s website at the link below. Copyright @ 2009 The Authors.The conserved Rho-type GTPase Cdc42p is a key regulator of signal transduction and polarity in eukaryotic cells. In the yeast Saccharomyces cerevisiae, Cdc42p promotes polarized growth through the p21-activated kinases Ste20p and Cla4p. Previously, we demonstrated that Ste20p forms a complex with Erg4p, Cbr1p and Ncp1p, which all catalyze important steps in sterol biosynthesis. CLA4 interacts genetically with ERG4 and NCP1. Furthermore, Erg4p, Ncp1p and Cbr1p play important roles in cell polarization during vegetative growth, mating and filamentation. As Ste20p and Cla4p are involved in these processes it seems likely that sterol biosynthetic enzymes and p21-activated kinases act in related pathways. Here, we demonstrate that the deletion of either STE20 or CLA4 results in increased levels of sterols. In addition, higher concentrations of steryl esters, the storage form of sterols, were observed in cla4Δ cells. CLA4 expression from a multicopy plasmid reduces enzyme activity of Are2p, the major steryl ester synthase, under aerobic conditions. Altogether, our data suggest that Ste20p and Cla4p may function as negative modulators of sterol biosynthesis. Moreover, Cla4p has a negative effect on steryl ester formation. As sterol homeostasis is crucial for cell polarization, Ste20p and Cla4p may regulate cell polarity in part through the modulation of sterol homeostasis.Deutsche Forschungsgemeinschaft and the Austrian FWF

Sensitivity to the KARMEN Timing Anomaly at MiniBooNE

We present sensitivities for the MiniBooNE experiment to a rare exotic pion decay producing a massive particle, Q^0. This type of decay represents one possible explanation for the timing anomaly reported by the KARMEN collaboration. MiniBooNE will be able to explore an area of the KARMEN signal that has not yet been investigated

The reaction np→ppπ−{n} {p} \to {p} {p} \pi^{-} from threshold up to 570 MeV

The reaction ${n} {p} \to {p} {p} \pi^{-}$ has been studied in a kinematically complete measurement with a large acceptance time-of-flight spectrometer for incident neutron energies between threshold and 570 MeV. The proton-proton invariant mass distributions show a strong enhancement due to the pp($^{1}{S}_{0}$) final state interaction. A large anisotropy was found in the pion angular distributions in contrast to the reaction ${p}{p} \to {p}{p} \pi^{0}$. At small energies, a large forward/backward asymmetry has been observed. From the measured integrated cross section $\sigma({n}{p} \to {\rm p}{p} \pi^{-})$, the isoscalar cross section $\sigma_{01}$ has been extracted. Its energy dependence indicates that mainly partial waves with Sp final states contribute. Note: Due to a coding error, the differential cross sections ${d \sigma}/{d M_{pp}}$ as shown in Fig. 9 are too small by a factor of two, and inn Table 3 the differential cross sections ${d \sigma}/{d \Omega_{\pi}^{*}}$ are too large by a factor of $10/2\pi$. The integrated cross sections and all conclusions remain unchanged. A corresponding erratum has been submitted and accepted by European Physics Journal.Comment: 18 pages, 16 figure

Analysing powers for the reaction n⃗p→ppπ−\vec{\rm n} {\rm p} \to {\rm p} {\rm p} \pi^{-} and for np elastic scattering from 270 to 570 MeV

The analysing power of the reaction ${\rm n}{\rm p} \to {\rm p}{\rm p} \pi^{-}$ for neutron energies between threshold and 570 MeV has been determined using a transversely polarised neutron beam at PSI. The reaction has been studied in a kinematically complete measurement using a time-of-flight spectrometer with large acceptance. Analysing powers have been determined as a function of the c.m. pion angle in different regions of the proton-proton invariant mass. They are compared to other data from the reactions ${\rm n}{\rm p} \to {\rm p}{\rm p} \pi^{-}$ and ${{\rm p}{\rm p} \to {\rm p}{\rm p} \pi^{0}}$. The np elastic scattering analysing power was determined as a by-product of the measurements.Comment: 12 pages, 6 figures, subitted to EPJ-

Search for Exotic Muon Decays

Recently, it has been proposed that the observed anomaly in the time distribution of neutrino induced reactions, reported by the KARMEN collaboration, can be interpreted as a signal from an exotic muon decay branch mu+ to e+ X. It has been shown that this hypothesis gives an acceptable fit to the KARMEN data if the boson X has a mass of m_X=103.9MeV/c^2, close to the kinematical limit. We have performed a search for the X particle by studying for the first time the very low energy part of the Michel spectrum in mu+ decays. Using a HPGe detector setup at the muE4 beamline at PSI we find branching ratios BR(mu+ to e+ X)<5.7e-4 (90% C.L.) for most of the region 103MeV/c^2<m_X<105MeV/c^2.Comment: 9 page

Infants’ perception of goal-directed actions: A multi-lab replication reveals that infants anticipate paths and not goals

Influential developmental theories claim that infants rely on goals when visually anticipating actions. A widely noticed study suggested that 11-month-olds anticipate that a hand continues to grasp the same object even when it swapped position with another object (Cannon, E., & Woodward, A. L. (2012). Infants generate goal-based action predictions. Developmental Science, 15, 292-298.). Yet, other studies found such flexible goal-directed anticipations only from later ages on. Given the theoretical relevance of this phenomenon and given these contradicting findings, the current work investigated in two different studies and labs, whether infants indeed flexibly anticipate an action goal. Study 1 (N = 144) investigated by means of five experiments, under which circumstances (e.g., animated agent, human agent) 12-month-olds show flexible goal anticipation abilities. Study 2 (N = 104) presented 11-, 32-month-olds and adults both a human grasping action as well as a non-human action. In none of the experiments did infants flexibly anticipate the action based on the goal, but rather on the movement path, irrespective of the type of agent. Although one experiment contained a direct replication of Cannon and Woodward (2012), we were not able to replicate their findings. Overall our work challenges the view that infants are able to flexibly anticipate action goals from early on, but rather rely on movement patterns when processing other's actions

Comparison of ultracold neutron sources for fundamental physics measurements

Ultracold neutrons (UCNs) are key for precision studies of fundamental parameters of the neutron and in searches for new CP violating processes or exotic interactions beyond the Standard Model of particle physics. The most prominent example is the search for a permanent electric dipole moment of the neutron (nEDM). We have performed an experimental comparison of the leading UCN sources currently operating. We have used a 'standard' UCN storage bottle with a volume of 32 liters, comparable in size to nEDM experiments, which allows us to compare the UCN density available at a given beam port.Comment: 20 pages, 30 Figure