Phenotypic characterisation of <i>hst-24</i>.

<p>(<b>A</b>) Dissected flower from developmental stage 15a from <i>hst-24/hws-1</i>. (<b>B</b>) Comparative analyses of sepal and petal sizes from flowers (stage 15a) of Col-0, <i>hws-1</i>, <i>hst-24/hws-1</i> and <i>hst-24</i>. (<b>C</b>). Twenty-five flowers from six plants of Col-0, <i>hst-24/hws-1</i> and <i>hst-24</i> were dissected and their sepals and petals quantified and statistically analysed by regression analyses using generalized linear models. Stars indicate a significant difference in the mean at P≤0.001 n = 450. Bars indicate SD. (<b>D</b>) Rosettes, and (<b>E</b>) Dissected leaves from 22-day-old plants from Col-0, <i>hws-1</i>, <i>hst-24/hws-1</i> and <i>hst-24</i>. Bars in A, B = 1mm; and in D, E = 1 cm.</p

Mean of sepal and petal numbers in Col-0, <i>hws-1</i>, <i>hst-24/hws-1</i> and <i>hst-24</i> in Col-0 from the first 25 flowers of the inflorescences, (flowers n = 200).

<p>Mean of sepal and petal numbers in Col-0, <i>hws-1</i>, <i>hst-24/hws-1</i> and <i>hst-24</i> in Col-0 from the first 25 flowers of the inflorescences, (flowers n = 200).</p

miRNA pathway and co-suppression between <i>hws-1</i> and miRNA pathway mutants.

<p>Single (<b>A, G, J, M, P, S</b>) <i>hws-1</i>, (<b>D</b>) <i>hws-2</i>, (<b>B</b>) <i>ddl-2</i>, (<b>E</b>) <i>se-1</i>, (<b>H</b>) <i>hyl-1</i>, (<b>K</b>) <i>dcl1-9</i>, (<b>N</b>) <i>hen1-5</i>, (<b>Q</b>) <i>hst-1</i>, (<b>T</b>) <i>ago1-37</i>, and double (<b>C</b>) <i>hws-1Xddl-2</i>, (<b>F</b>) <i>hws-1Xse-1</i>, (<b>I</b>) <i>hws-1Xhyl-1</i>, (<b>L</b>) <i>hws-1Xdcl1-9</i>, (<b>O</b>) <i>hws-1Xhen1-5</i>, (<b>R</b>) <i>hws-1Xhst-1</i>, (<b>U</b>) <i>hws-1Xago1-37</i>, mutants showing co-suppression of phenotypes. Bars = 1mm. The (<b>V</b>) miRNA pathway (modified from [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189788#pone.0189788.ref032" target="_blank">32</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189788#pone.0189788.ref036" target="_blank">36</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189788#pone.0189788.ref061" target="_blank">61</a>]) has been included for reference.</p

The <i>shs-2</i> and <i>shs-3</i> mutants are alleles of <i>HST</i>.

<p>(<b>A-H</b>), Aerial and (<b>I-P</b>), lateral views of flowers at stage 15a; and (<b>Q-X</b>), lateral view of mature green siliques from wild type in Col-0, <i>hws-1</i>, <i>shs-2/hws-1</i> (<i>hst-23/hws-1</i>), <i>shs-2</i> (<i>hst-23)</i>, <i>shs-3/hws-1</i> (<i>hst-24/hws-1</i>), <i>shs-3</i> (<i>hst-24)</i>, <i>hws-1xhst-1</i>, <i>hst-1</i>. Bars = 1mm. (<b>Y</b>), Mapping strategy used to identify the <i>hst-23 and hst-24</i> mutations. Structure of the gene and location of the transition substitution (C.G→T.A) at positions 4.587 Kb and 5.517 Kb in <i>hst-23</i> and (G.C→A.T) at 0.583 Kb in <i>hst-24</i> from the ATG are included, intragenic regions are represented by thin lines and exons by dark boxes.</p