Saffron (Crocus sativus L.) extract prevents and improves D-galactose and NaNO2 induced memory impairment in mice

This study was conducted to examine the effects of saffron extract on preventing D-galactose and NaNO2 induced memory impairment and improving learning and memory deficits in amnestic mice. In this study, the learning and memory functions in ovariectomized mice were examined by the one way passive and active avoidance tests. In active avoidance test, training in amnestic treated (AT) and amnestic prophylaxis (AP) groups, was improved so that there was a significant difference between them and the amnestic control (AC) group. In passive avoidance test, animal’s step through latency, as an index for learning, in all test groups was significantly greater than control group. Total time spent in dark room (DS), which opposes the memory retention ability, in AC was significantly greater than AT group at 1 and 2 hours after full training, while there was not any significant difference between this index in AP and AT as compared with normal control (NC) group. Our findings indicate that saffron hydroalcoholic extract prevents and improves amnesia induced by D-galactose and NaNO2 in mice

Development of a Testing and Analysis Framework for Validation of Rehabilitating Pipe-in-Pipe Technologies

Aging natural gas pipeline infrastructure needs rehabilitation, and trenchless, pipe-in-pipe (PIP) technologies offer a versatile solution. For example, legacy cast/wrought iron pipes have been subject to elevated incident rates for decades (www.phmsa.dot.gov). In an effort to accelerate innovation, the United States (U.S.) Department of Energy (DOE) has invested in a recently initiated, 3-year research program focused on pipeline “REPAIR”. To establish industry adoption of new technologies, a robust framework to evaluate and validate systems under in-service loading conditions is required. This paper introduces the approach taken by the Testing and Analysis team to develop a framework that confirms a 50-year design life for the PIP technologies. Testing protocols involve a comprehensive literature review, performance criteria, and relevant load cases and failure modes of PIP technologies. We use numerical and analytical modelling to investigate failure modes and severe conditions, thus informing testing protocols. In this paper, we discuss analytical frameworks and proposed model validation methods. We further discuss plans for test geometries (e.g., circumferentially cracked host pipe) and protocols (e.g., cyclic/dynamic traffic loading) to apply relevant load cases and probe failure modes in service. Modifications and enhancements are investigated in light of the insights gained from review and modelling. The testing and analysis framework for validating service life performance of trenchless PIP repair methods is intended to accelerate the development and adoption of new and safe repair technologies in the gas industry, as well as other critical lifeline systems.Patrick G. Dixon, Brad P. Wham, Jacob Klingaman, Allan Manalo, T. Tafsirojjaman, Khalid Farrag, Thomas D. O, Rourke, Mija H. Hubler, Shideh Dasht

Expression analysis of vimentin and the related lncRNA network in breast cancer

Vimentin (VIM) is a mesenchymal marker which is expressed in some cancer types including breast cancer. A long non-coding RNA (lncRNA) has been identified to be transcribed from VIM gene locus and positively regulate expression of VIM. This lncRNA has been named as VIM-antisense 1 (VIM-AS1). Expression of VIM is also regulated by another lncRNA namely AGAP2-antisense RNA 1 (AGAP2-AS1). In the current study, we aimed at identification of the expression pattern of VIM, VIM-AS1, AGAP2 and AGAP2-AS1 in 78 breast cancer samples and their paired adjacent non-cancerous tissues (ANCTs) by means of real time PCR. All mentioned genes were significantly down-regulated in tumoral tissues compared with ANCTs (P values less than 0.000). Relative expression of VIM-AS1 in tumoral tissues versus ANCTs was associated with menopause age (P = .02) in a way that this gene was down-regulated in most of patients whose menopause age was between 40 and 50 years. Moreover, AGAP2�AS1 relative expression was associated with patients' body mass index (P = .03). There were trends toward association between VIM relative expression and tumor size (P = .07) and association between VIM-AS1 relative expression and obesity (P = .06). Expression of VIM was significantly higher in tumoral tissues of patients who had history of hormone replacement therapy compared with those without such history (P = .03). Moreover, expression levels of both VIM and AGAP2-AS1 were lower in patients whose menarche age was between 10 and 12 years old compared with those whose menarche age was between 13 and 15 years old (P values = .01 and 0.04, respectively). Transcript quantities of VIM, VIM-AS1, AGAP2 and AGAP2-AS1 were correlated with each other both in tumoral tissues and in ANCTs. Among four assessed genes, AGAP2 had the best diagnostic power for discrimination of tumoral tissues from ANCTs (AUC value = 0.87). Combination of four genes led to enhancement of AUC value to 0.94. The current study shows the importance of VIM and its associated lncRNAs in breast cancer and potentiates these genes as biomarkers for this malignancy. Moreover, these lncRNAs might be regarded as therapeutic targets in breast cancer. © 202

Frequency and risk factors of penicillin and amoxicillin allergy in suspected patients with drug allergy

Background: Unconfirmed beta-lactam allergy is a significant public health problem because of the limitations it imposes in drug selection. In this study, we aimed to evaluate patients referred for beta-lactam allergy to determine the frequency of confirmed beta-lactam allergy and identify some risk factors. Methods: In a prospective cohort study, all referred patients to Immunology, Asthma and Allergy Research Institute in Tehran University of Medical Sciences (between 2007 - 2009) who suspected to have beta-lactam allergy were entered into this study based on having the inclusion criteria. Follow-up was performed 6-8 years after the final diagnosis. Diagnosis of beta-lactam allergy relies on thorough history and specific IgE measurements (ImmunoCAP), skin prick testing (SPT), intradermal testing (IDT), patch testing, and oral drug challenge test. Results: Fifty-one patients with mean age of 24.5 (±18.5) years were enrolled in this study. Based on workups, beta-lactam allergy was confirmed in 16 (31.4%) patients, suspicious in 22 (43.1%) patients and ruled out in 13 (25.5%) patients. During the follow-up, 3 patients with suspicious drug allergy consumed the culprit drug with no reaction so allergy was finally ruled out in 16 (31.4%) patients. Age, sex, atopy and family history of drug allergies were not significantly different between the patients with confirmed or ruled-out diagnosis of penicillin and amoxicillin allergy. Conclusion: At least up to one-third of patients with a history of beta-lactam allergy are proven to be safe using the drug. Also, a clear protocol consists ofserum slgE assay and SPT can be helpful to the physicians in the health care system. © 2017, Academy of Medical Sciences of I.R. Iran. All rights reserved