Characteristics of similarity search of unigenes against Nr and Swiss-Prot databases.

<p>(A) E-value distribution of BLAST hits for each unigene with an E-value threshold of 10^-5 in Nr database. (B) E-value distribution of BLAST hits for each unigene with an E-value threshold of 10^-5 in Swiss-Prot database. (C) Similarity distribution of the top BLAST hits for each unigene in Nr database. (D) Similarity distribution of the top BLAST hits for each unigene in Swiss-Prot database.</p

Frequency of classified repeat types of SSRs.

<p>The AG/CT di-nucleotide repeat motif was the most abundant one detected in our SSRs.</p

Clusters of orthologous groups (COG) classification of wax gourd transcriptome.

<p>All the unigenes were aligned to COG database to predict and classify possible functions. In total 23, 977 of the 36, 070 unigenes with Nr hits were grouped into 25 COG classifications.</p

Comparison of wax gourd unigenes to orthologous <i>C. lanatus</i> coding sequences.

<p>(A) The ratio of wax gourd unigene length to <i>C. lanatus</i> ortholog length was plotted against wax gourd unigene coverage depth. Overall, there are 3, 227 unigenes with the ratio greater than 1, and 25, 763 unigenes with the ratio less than 1. (B) Total percent of <i>C. lanatus</i> ortholog coding sequence that was covered by all wax gourd unigenes. In total, 8, 936 orthologs could be covered by unigenes with a percentage of more than 80%, and the cover percentage of around 4, 796 orthologs ranged from 40% to 80%. Furthermore, 827 orthologs were covered with only 20% or lower.</p

Assessment of assembly quality.

<p>Distribution of unique-mapped reads of the assembled unigenes.</p

Examples of polymorphic products amplified by SSR primer pairs.

<p>A–D. PCR products amplified by primer pair 1, 2, 19 and 35, respectively. 1-6 represent B94, B96, B214, B318, P4, P75, respectively.</p

El limbo: Año I Número 12 - 1921 abril 23

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200

Image_1_Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How).jpeg

Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been reported until now. Here, we used an F2 population from the cross between the gynoecious line ‘A36’ and the monoecious line ‘SX’ for genetic mapping and revealed that chieh-qua gynoecy was regulated by a single recessive gene. We fine-mapped it into a 530-kb region flanked by the markers Indel-3 and KASP145 on Chr.8, which harbors eight candidate genes. One of the candidate genes, Bhi08G000345, encoding networked protein 4 (CqNET4), contained a non-synonymous SNP resulting in the amino acid substitution of isoleucine (ATA; I) to methionine (ATG; M). CqNET4 was prominently expressed in the female flower, and only three genes related to ethylene synthesis were significantly expressed between ‘A36’ and ‘SX.’ The results presented here provide support for the CqNET4 as the most likely candidate gene for chieh-qua gynoecy, which differed from the reported gynoecious genes.</p

Table_2_Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How).xlsx

Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been reported until now. Here, we used an F2 population from the cross between the gynoecious line ‘A36’ and the monoecious line ‘SX’ for genetic mapping and revealed that chieh-qua gynoecy was regulated by a single recessive gene. We fine-mapped it into a 530-kb region flanked by the markers Indel-3 and KASP145 on Chr.8, which harbors eight candidate genes. One of the candidate genes, Bhi08G000345, encoding networked protein 4 (CqNET4), contained a non-synonymous SNP resulting in the amino acid substitution of isoleucine (ATA; I) to methionine (ATG; M). CqNET4 was prominently expressed in the female flower, and only three genes related to ethylene synthesis were significantly expressed between ‘A36’ and ‘SX.’ The results presented here provide support for the CqNET4 as the most likely candidate gene for chieh-qua gynoecy, which differed from the reported gynoecious genes.</p

Image_3_Fine mapping and candidate gene analysis of gynoecy trait in chieh-qua (Benincasa hispida Cogn. var. chieh-qua How).jpeg

Gynoecy demonstrates an earlier production of hybrids and a higher yield and improves the efficiency of hybrid seed production. Therefore, the utilization of gynoecy is beneficial for the genetic breeding of chieh-qua. However, little knowledge of gynoecious-related genes in chieh-qua has been reported until now. Here, we used an F2 population from the cross between the gynoecious line ‘A36’ and the monoecious line ‘SX’ for genetic mapping and revealed that chieh-qua gynoecy was regulated by a single recessive gene. We fine-mapped it into a 530-kb region flanked by the markers Indel-3 and KASP145 on Chr.8, which harbors eight candidate genes. One of the candidate genes, Bhi08G000345, encoding networked protein 4 (CqNET4), contained a non-synonymous SNP resulting in the amino acid substitution of isoleucine (ATA; I) to methionine (ATG; M). CqNET4 was prominently expressed in the female flower, and only three genes related to ethylene synthesis were significantly expressed between ‘A36’ and ‘SX.’ The results presented here provide support for the CqNET4 as the most likely candidate gene for chieh-qua gynoecy, which differed from the reported gynoecious genes.</p