Total and CO-reactive heme content of actinorhizal nodules and the roots of some non-nodulated plants

The concentration of total and CO-reactive heme was measured in actinorhizal nodules from six different genera. This gave the upper limit to hemoglobin concentration in these nodules. Quantitative extraction of CO-reactive heme was achieved under anaerobic conditions in a buffer equilibrated with CO and containing Triton X-100. The concentration of CO-reactive heme in nodules of Casuarina and Myrica was approximately half of that found in legume nodules, whereas in Comptonia, Alnus and Ceanothus the concentrations of heme were about 10 times lower than in legume nodules. There was no detectable CO-reactive heme in Datisca nodules, but low concentrations were detected in roots of all non-nodulating plants examined, including Zea mays . Difference spectra of CO treated minus dithionite-reduced extracts displayed similar wavelengths of maximal and minimal light absorption for all extracts, and were consistent with those of a hemoglobin. The concentration of CO-reactive heme was not correlated to the degree to which CO inhibited nitrogenase activity nor was it affected by reducing the oxygen concentration in the rooting zone. However, there was a positive correlation between heme concentration and suberization or lignification of the walls of infected host cells. These observations demonstrate that, unlike legume nodules, high concentrations of heme or hemoglobin are not needed for active nitrogen fixation in most actinorhizal nodules. Nonetheless, a significant amount of CO-reactive heme is found in the nodules of Alnus, Comptonia, and Ceanothus, and in the roots of Zea mays . The identity and function of this heme is unknown.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43460/1/11104_2006_Article_BF02370943.pd

Effects of heparinoids on bovine aortic smooth muscle cells

Smooth muscle cells (SMCs) comprise the bulk of the tunica media of all blood vessels. Upon injury to the overlying endothelial monolayer of blood vessels, SMCs proliferate into the lumen of the vessel and cause dangerous occlusions. The glycosaminoglycan (GAG) heparin has been shown to be antiproliferative towards SMCs both in vitro and in vivo. The precise molecular mechanism whereby heparin exerts this antiproliferative effect is unknown. The effect of various lengths of heparin treatment on endogenous proteoglycan metabolism in both log and confluent cells was examined. The majority of GAG produced by SMCs is chondroitin sulfate (CS) or dermatan sulfate (DS). Most (90%) of the GAG produced by SMCs is secreted to the culture media. Heparin treatment caused a decrease in the amount of proteoglycans produced, and secreted to the culture media by log cells, while a stimulation of proteoglycan synthesis was observed in confluent cells. Heparin treatment had little effect on the overall type or distribution of proteoglycans in either log or confluent SMCs. Heparin treatment also had little effect on the synthesis of DNA, RNA, or protein by SMCs. The binding and uptake of heparin into SMCs was examined in both log and confluent cells. It was found that log cells exhibit a K\sb{\rm d} of 171 nM for heparin and have 1.5 $\times$ 10\sp5 binding sites/cell for heparin. Confluent SMCs have a K\sb{\rm d} of 263 nM for heparin and have 7.9 $\times$ 10\sp5 binding sites/cell for heparin. Pre-treatment of cells with heparin prior to binding experiments showed a marked change in both receptor affinity and number of binding sites in log cells. Confluent cells exhibited a decrease in the number of binding sites/cell, but no change in receptor affinity. The binding of heparin to SMCs was examined further through the use of heparin analogs to compete with heparin binding. Heparin was depolymerized by either HONO degradation or periodate oxidation, and these fragments were screened for their ability to compete with heparin binding. Uptake of heparin into SMCs was observed in cells with and without heparin treatment. It was found that heparin-treated cells took up less heparin than untreated cells. The effect of xyloside GAG synthesis initiators were tested in SMCs. Estradiol xyloside gives the same level of stimulation in GAG synthesis (3 to 4-fold) at a 20 fold lower concentration than p-nitrophenyl-xyloside. However, the type of GAG produced by SMCs was unchanged with the xyloside treatment.U of I OnlyETDs are only available to UIUC Users without author permissio