SyntenyBlockData.tar

These files contain the coordinates of synteny blocks calculated from GenAlyzer match results. These were used to prepare the figures for segmentation (i.e. Figs 2, S1-S9)

Baseline characteristics at the time of sampling in term infants, preterm infants sampled at birth and preterm infants sampled at term equivalent age.

<p>Data are presented as mean (95% CI).</p

A summary of studies to date that have assessed telomere length in preterm infants in comparison to term born infants or age matched fetus’.

<p>A summary of studies to date that have assessed telomere length in preterm infants in comparison to term born infants or age matched fetus’.</p

Preterm infants have significantly longer telomeres than their term born counterparts - Fig 3

<p>Relationship between relative telomere length (T/S ratio) and gestational age (a), birth weight (b) and maternal age (c).</p

Gender distribution and mean (95% CI) T/S ratios of male and female infants included in the study overall and within each study cohort.

<p><i>p</i> value signifies results from univariate ANOVA analyses of T/S ratios in males compared to females overall and within each cohort. (*Gender data missing in 2/31 term infants).</p

Relative telomere length (T/S ratio) in preterm infants at birth, preterm infants at term age and term born infants.

<p>Data are Mean (95% CI).</p

Epigenetic regulator expression in individual human blastocysts by qPCR (n = 12 replicates for each group).

<p>Ct values were normalized to <i>RPL19</i>, an internal, constant housekeeping gene. Fold change was determined using the ΔΔCt method on the average of technical duplicates. Error bars represent standard error and the y-axis denotes fold change between euploid and aneuploidy. <b>A)</b> The expression of DNA methylatransferases was analyzed in euploid, trisomy 15, and monosomy 15 blastocysts (*P < 0.05). <b>B)</b> The expression of post-translational regulators and the chromatin modifying protein, PRMT5 (*P < 0.05).</p

Primer information and qPCR efficiencies for chromosomes 15, 22, and housekeeping genes.

<p>Primer information and qPCR efficiencies for chromosomes 15, 22, and housekeeping genes.</p

Developmental gene expression in individual human blastocysts (n = 10 replicates for each group) was performed by qPCR.

<p>Ct values were normalized to <i>PPIA</i>, an internal, constant housekeeping gene. Fold change was determined using the ΔΔCt method on the average of technical duplicates. Error bars represent standard error and the y-axis denotes fold change between euploid and aneuploidy. <b>A)</b> Signigicant decreased expression of chromosome 15 genes in monosomy 15 blastocysts compared to controls (*P < 0.05). <b>B)</b> Significant decreased expression of chromosome 22 genes in monosomy 22 blastocysts compared to controls (*P < 0.05).</p

Methylome profiles of pooled human blastocysts (n = 10 each pool with 2–3 replicates per group).

<p>Beta value reflects the level of global methylation with no variation observed in association with blastocyst chromosome constitution. (no statistical significance). Standard deviation (STDEV) was calculated for each group, reflecting low biological variability between replicates.</p