Real-time Information, Uncertainty and Quantum Feedback Control

Feedback is the core concept in cybernetics and its effective use has made great success in but not limited to the fields of engineering, biology, and computer science. When feedback is used to quantum systems, two major types of feedback control protocols including coherent feedback control (CFC) and measurement-based feedback control (MFC) have been developed. In this paper, we compare the two types of quantum feedback control protocols by focusing on the real-time information used in the feedback loop and the capability in dealing with parameter uncertainty. An equivalent relationship is established between quantum CFC and non-selective quantum MFC in the form of operator-sum representation. Using several examples of quantum feedback control, we show that quantum MFC can theoretically achieve better performance than quantum CFC in stabilizing a quantum state and dealing with Hamiltonian parameter uncertainty. The results enrich understanding of the relative advantages between quantum MFC and quantum CFC, and can provide useful information in choosing suitable feedback protocols for quantum systems.Comment: 24 page

O-Antigen Gene Clusters of Plesiomonas shigelloides Serogroups and Its Application in Development of a Molecular Serotyping Scheme

Plesiomonas shigelloides is a Gram-negative, flagellated, rod-shaped, ubiquitous, and facultative anaerobic bacterium. It has been isolated from various sources, such as freshwater, surface water, and many wild and domestic animals. P. shigelloides is associated with diarrheal diseases of acute secretory gastroenteritis, an invasive shigellosis-like disease, and a cholera-like illness in humans. At present, 102 somatic antigens and 51 flagellar antigens of P. shigelloides have been recognized; however, very little is known about variations of O-antigens among P. shigelloides species. In this study, 12 O-antigen gene clusters of P. shigelloides, O2H1a1c (G5877), O10H41 (G5892), O12H35 (G5890), O23H1a1c (G5263), O25H3 (G5879), O26H1a1c (G5889), O32H37 (G5880), O33H38 (G5881), O34H34 (G5882), O66H3 (G5270), O75H34 (G5885), and O76H39 (G5886), were sequenced and analyzed. The genes that control O-antigen synthesis are present as chromosomal gene clusters that maps between rep and aqpZ, and most of the synthesis and translocation of OPS (O-specific polysaccharide) belongs to Wzx/Wzy pathway with the exception of O12, O25, and O66, which use the ATP-binding cassette (ABC) transporter pathway. Phylogenetic analysis of wzx and wzy show that the wzx and wzy genes are specific to individual O-antigens and can be used as targets in molecular typing. Based on the sequence data, an O-antigen specific suspension array that detects 12 distinct OPS’ has been developed. This is the first report to catalog the genetic features of P. shigelloides O-antigen variations and develop a suspension array for the molecular typing. The method has several advantages over traditional bacteriophage and serum agglutination methods and lays the foundation for easier identification and detection of additional O-antigen in the future

RpoE Facilitates Stress-Resistance, Invasion, and Pathogenicity of Escherichia coli K1

Escherichia coli K1 is the most common Gram-negative bacterium that causes neonatal meningitis; thus, a better understanding of its pathogenic molecular mechanisms is critical. However, the mechanisms by which E. coli K1 senses the signals of the host and expresses toxins for survival are poorly understood. As an extracytoplasmic function sigma factor, RpoE controls a wide range of pathogenesis-associated pathways in response to environmental stress. We found that the ΔrpoE mutant strain reduced the binding and invasion rate in human brain microvascular endothelial cells (HBMECs) in vitro, level of bacteremia, and percentage of meningitis in vivo. To confirm the direct targets of RpoE in vivo, we performed qRT-PCR and ChIP-qPCR on known toxic genes. RpoE was found to regulate pathogenic target genes, namely, ompA, cnf1, fimB, ibeA, kpsM, and kpsF directly and fimA, aslA, and traJ indirectly. The expression of these genes was upregulated when E. coli K1 was cultured with antibacterial peptides, whereas remained unchanged in the presence of the ΔrpoE mutant strain. Moreover, RpoE reduced IL-6 and IL-8 levels in E. coli K1-infected HBMECs. Altogether, these findings demonstrate that RpoE mediates the host adaptation capacity of E. coli K1 via a regulatory mechanism on virulence factors

Genetic diversity of K-antigen gene clusters of E. coli and their molecular typing using a suspension array

Capsular polysaccharides (CPSs), or K-antigens, are the major surface antigens of Escherichia coli. More than 80 serologically unique K-antigens are classified into four groups (Groups 1â 4) of capsules. Group 1 and 4 contain the Wzy-dependent polymerization pathway and the gene clusters are in the order galF to gnd; Group 2 and 3 contain the ABC transporter-dependent pathway and the gene clusters consist of three regions, region 1, 2 and 3. Little is known about the variations among the gene clusters. In this study, nine serotypes of K-antigen gene clusters (K2ab, K11, K20, K24, K38, K84, K92, K96, and K102), were sequenced and correlated with their CPS chemical structures. Based on the sequence data, a K-antigen specific suspension array that detects 10 distinct CPSs, including the above nine CPSs plus K30, was developed. This is the first report to catalog the genetic features of E. coli K-antigen variations, and to develop a suspension array for their molecular typing. The method has a number of advantages over traditional bacteriophage and serum agglutination methods, and lays the foundation for straightforward identification and detection of additional K-antigens in the future.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author