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Characterization of Bloomy Rind Cheese Recipes and the Impact of High Pressure Processing (HPP) on Cheese Quality
Bloomy rind cheeses, including Camembert and Brie type cheeses, are highly susceptible to contamination by environmental pathogens during their manufacture and ripening. These cheeses undergo many physiochemical changes during ripening that provides these pathogens with a suitable environment to grow. One example of this change is an increase of pH to greater than 7 during the initial stages of the cheese ripening. The risk associated with this cheese type has been well documented and people with compromised immune systems are advised against consuming these cheeses. Currently, there are no options available for bloomy rind cheese producers to add a kill step in late in the manufacture/ripening of bloomy rind cheeses. Due to the lack of kill step, cheese makers are forced to rely on stringent sanitation techniques and environmental monitoring to mitigate the risk of contamination.
There are many ways to manufacture bloomy rind cheeses, and we hypothesized that different cheese recipes would perform differently under HPP condition with the stabilized cheeses being most likely to withstand this treatment with minimal impact on the overall quality. To be able to test this hypothesis we first manufactured five varieties of bloomy rind cheeses to investigate the composition and characteristics of the recipes as a baseline for the expected quality from these different bloomy rind cheese varieties. Next, we evaluated the effect of HPP on cheese quality for selected cheese varieties. Finally, HPP was evaluated for the overall reduction of Listeria monocytogenes. The overall hypothesis of this research is that HPP (high hydrostatic pressure processing) could be effectively used on bloomy rind cheeses as a post-manufacture kill step, and that the cheese making practices used will influence how the cheeses withstand HPP.
Camembert cheese varieties varied in composition and characteristics that were influenced by the cheese making practices. The most notable difference between the varieties was the paste stability. Paste stability is defined by the distance that the center of the cheese is displaced from the rind after slicing. Another notable observation is the variation in total Ca content, which is related to the amount of colloidal calcium phosphate that is associated with the protein matrix that retains the cheese structure. When determining the effect of HPP on cheese quality, the most notable discovery was the destruction of the mycelium from the surface fungi used to ripen these cheeses. HPP treatment caused significant discoloration, from white to brown/yellow, and would be unacceptable to consumers of bloomy rind cheeses. Finally, when evaluating the reduction of L. monocytogenes using HPP there were large reductions (> 5-log CFU/g) in the microbial load at greater than 450 MPa for 10 minutes.
Based on the evidence provided, HPP successfully reduces L. monocytogenes in Camembert; however, the tested treatments produced unacceptable changes in cheese appearance. Until a technology is found that can be successfully applied to bloomy rind cheeses, cheese makers must emphasize proper sanitation procedures and environmental monitoring to control the risk of contamination
âI didnât have any optionâ: Experiences of people receiving in-centre haemodialysis during the COVID-19 pandemic
People receiving in-centre haemodialysis (ICHD) during the COVID-19 pandemic had to adjust to more challenging treatment conditions. To explore peopleâs experiences of adjustment to ICHD during the pandemic. Thematic analysis of in-depth, semi-structured interviews with 14 adult UK ICHD patients.
Findings: Four themes were identified: âperceptions of the threatâ, âimpacts on treatmentâ, âimpaired communicationâ and âcoping and positive adjustmentâ. These described participantsâ experiences of vulnerability to COVID-19; the ways the pandemic affected dialysis and clinical care; the impact that measures to reduce viral transmission had on communication and interaction within dialysis units; and ways that participants coped and made positive adjustments to the adversities imposed by the pandemic. The findings give insights into adjustment during extreme adversity. They also help to identify ways that support for ICHD patients could be improved as pandemic conditions recede, and ways that dialysis units could prepare for future outbreaks of infectious illness
Human Microglia Transplanted in Rat Focal Ischemia Brain Induce Neuroprotection and Behavioral Improvement
BACKGROUND AND PURPOSE: Microglia are resident immunocompetent and phagocytic cells of central nervous system (CNS), which produce various cytokines and growth factors in response to injury and thereby regulate disease pathology. The purpose of this study is to investigate the effects of microglial transplantation on focal cerebral ischemia model in rat. METHODS: Transient middle cerebral artery occlusion (MCAO) in rats was induced by the intraluminal filament technique. HMO6 cells, human microglial cell line, were transplanted intravenously at 48 hours after MCAO. Functional tests were performed and the infarct volume was measured at 7 and 14 days after MCAO. Migration and cell survival of transplanted microglial cells and host glial reaction in the brain were studied by immunohistochemistry. Gene expression of neurotrophic factors, cytokines and chemokines in transplanted cells and host rat glial cells was determined by laser capture microdissection (LCM) and quantitative real time-PCR. RESULTS: HMO6 human microglial cells transplantation group demonstrated significant functional recovery compared with control group. At 7 and 14 days after MCAO, infarct volume was significantly reduced in the HMO group. In the HMO6 group, number of apoptotic cells was time-dependently reduced in the infarct core and penumbra. In addition, number of host rat microglia/macrophages and reactive astrocytes was significantly decreased at 7 and 14 days after MCAO in the penumbra. Gene expression of various neurotrophic factors (GDNF, BDNF, VEGF and BMP7) and anti-inflammatory cytokines (IL4 and IL5) was up-regulated in transplanted HMO6 cells of brain tissue compared with those in culture. The expression of GDNF and VEGF in astrocytes in penumbra was significantly up-regulated in the HMO6 group. CONCLUSIONS: Our results indicate that transplantation of HMO6 human microglial cells reduces ischemic deficits and apoptotic events in stroke animals. The results were mediated by modulation of gliosis and neuroinflammation, and neuroprotection provided by neurotrophic factors of endogenous and transplanted cells-origin
RIG-I contributes to the innate immune response after cerebral ischemia
BACKGROUND: Focal cerebral ischemia induces an inflammatory response that when exacerbated contributes to deleterious outcomes. The molecular basis regarding the regulation of the innate immune response after focal cerebral ischemia remains poorly understood. METHODS: In this study we examined the expression of retinoic acid-inducible gene (RIG)-like receptor-I (RIG-I) and its involvement in regulating inflammation after ischemia in the brain of rats subjected to middle cerebral artery occlusion (MCAO). In addition, we studied the regulation of RIG-I after oxygen glucose deprivation (OGD) in astrocytes in culture. RESULTS: In this study we show that in the hippocampus of rats, RIG-I and IFN-α are elevated after MCAO. Consistent with these results was an increased in RIG-I and IFN-α after OGD in astrocytes in culture. These data are consistent with immunohistochemical analysis of hippocampal sections, indicating that in GFAP-positive cells there was an increase in RIG-I after MCAO. In addition, in this study we have identified n-propyl gallate as an inhibitor of IFN-α signaling in astrocytes. CONCLUSION: Our findings suggest a role for RIG-I in contributing to the innate immune response after focal cerebral ischemia
Breeding systems in Tolpis (Asteraceae) in the Macaronesian islands: the Azores, Madeira and the Canaries
Plants on oceanic islands often originate from
self-compatible (SC) colonizers capable of seed set by self fertilization. This fact is supported by empirical studies,
and is rooted in the hypothesis that one (or few) individuals
could find a sexual population, whereas two or more would
be required if the colonizers were self-incompatible (SI).
However, a SC colonizer would have lower heterozygosity
than SI colonizers, which could limit radiation and diver sification of lineages following establishment. Limited
evidence suggests that several species-rich island lineages
in the family Asteraceae originated from SI colonizers with
some ââleakinessââ (pseudo-self-compatibility, PSC) such
that some self-seed could be produced. This study of Tolpis
(Asteraceae) in Macaronesia provides first reports of the
breeding system in species from the Azores and Madeira,
and additional insights into variation in Canary Islands.
Tolpis from the Azores and Madeira are predominately SI
but with PSC. This study suggests that the breeding sys tems of the ancestors were either PSC, possibly from a
single colonizer, or from SI colonizers by multiple dis seminules either from a single or multiple dispersals. Long distance colonists capable of PSC combine the advantages
of reproductive assurance (via selfing) in the establishment
of sexual populations from even a single colonizer with the
higher heterozygosity resulting from its origin from an
outcrossed source population. Evolution of Tolpis on the
Canaries and Madeira has generated diversity in breeding
systems, including the origin of SC. Macaronesian Tolpis is
an excellent system for studying breeding system evolution
in a small, diverse lineage.info:eu-repo/semantics/publishedVersio
Describing congenital heart disease by using three-part segmental notation
The segmental classification of congenital heart disease, first conceptualized nearly 50 years ago, is now well established. The Van Praagh classification system, in particular, is commonly used throughout North America to facilitate communication between physicians from various specialties who are involved in diagnosing and managing congenital cardiovascular abnormalities. In the Van Praagh system, a three-part notation consisting of letters separated by commas and encompassed by a set of braces is used to succinctly describe the visceroatrial situs, the orientation of the ventricular loop, and the position and relation of the great vessels. For example, the notation "{S, D, S}" describes the normal anatomic configuration, in which the morphologic right atrium and largest hepatic lobe are on the patient's right side and the morphologic left atrium, stomach, and spleen are on the left side (situs solitus); the ventricular loop is curved rightward (dextro- or d-loop); and the aorta is posterior to and rightward of the main pulmonary artery (situs solitus). Because the Van Praagh notation imposes on its users a systematic approach to anatomic description, it is a helpful device for structuring the interpretation of imaging studies as well as the reporting of results: First, the morphologic right and left atria and ventricles must be identified; next, the visceroatrial situs and ventricular loop orientation may be determined from the positions of the cardiac chambers; and last, the position and relation of the great vessels (normal, inverted, transposed, or malpositioned) can be established. The article provides concise, step-by-step guidance for applying the Van Praagh system in the radiology reading room
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