From parasite genomes to one healthy world: Are we having fun yet?

In 1990, the Human Genome Sequencing Project was established. This laid the ground work for an explosion of sequence data that has since followed. As a result of this effort, the first complete genome of an animal, Caenorhabditis elegans was published in 1998. The sequence of Drosophila melanogaster was made available in March, 2000 and in the following year, working drafts of the human genome were generated with the completed sequence (92%) being released in 2003. Recent advancements and next-generation technologies have made sequencing common place and have infiltrated every aspect of biological research, including parasitology. To date, sequencing of 32 apicomplexa and 24 nematode genomes are either in progress or near completion, and over 600k nematode EST and 200k apicomplexa EST submissions fill the databases. However, the winds have shifted and efforts are now refocusing on how best to store, mine and apply these data to problem solving. Herein we tend not to summarize existing X-omics datasets or present new technological advances that promise future benefits. Rather, the information to follow condenses up-to-date-applications of existing technologies to problem solving as it relates to parasite research. Advancements in non-parasite systems are also presented with the proviso that applications to parasite research are in the making

The effects of lengthening the school day on female labor supply: Evidence from a quasi-experiment in Chile

In 1996, the Chilean government approved the extension of the school day, increasing the amount of time that students spend at school by 30%. Using data from the Chilean socio- economic household survey and administrative data from the Ministry of Education for 1990- 2006, we exploit the quasi-experimental nature of the reform’s implementation by time and region in order to identify the causal impact of the program on labor participation, employment and hours worked for women between 20 and 65 years old. The identification strategy relies on a fixed effect model of repeated cross-section. The results show a positive and significant effect on labor participation and female employment in all age groups and a negative and statistically significant effect on the number of hours worked. The main conclusion of this study is that the implicit childcare subsidy induced by the program had a positive and significant impact on the labor supply of women in Chile.Female labor supply; childcare; fertility; labor supply; Chile.

A multiplex PCR assay for differentiating economically important gastrointestinal nematodes of cattle

A multiplex polymerase chain reaction (PCR) test was developed for identifying gastrointestinal (GI) nematodes that commonly infect cattle. This assay was developed using adult-derived genomic DNA and shown capable of discriminating parasite eggs from the feces of experimentally-infected animals at both the species and genus levels. Sequence data from internal (ITS) and external (ETS) transcribed spacers of the ribosomal DNA (rDNA) repeats as well as the 3′-end of the small subunit rDNA and 5′-end of the large subunit rDNA were used to generate five primer sets which, when used simultaneously in a multiplex PCR, produce a unique electrophoretic DNA banding pattern charac- terized by a single DNA fragment for Ostertagia ostertagi (257 bp), Haemonchus placei (176 bp),Oesophagostomum radiatum (329 bp), Trichostrongylus colubriformis (243 bp) and Cooperia on- cophora (151 bp). In a similar manner, the constructed primer sets amplified DNA from Ostertagia lyrata, Haemonchus contortus, Trichostrongylus axei, Cooperia surnabada and Cooperia punctata. With respect to H. contortus, a closely migrating doublet was generated suggesting size heterogene- ity in the ETS which is consistent with multiple rDNA repeat units within this species. PCR analyses using mixtures of monospecifically-purified nematode eggs indicated a sensitivity of less than 0.5 egg-DNA equivalent per species. Although, not designed as a quantitative technique, relative PCR signal intensities corresponded to relative egg burdens within the DNA samples from mixed species of eggs. Published by Elsevier Science B.V

Patterning molecular scale paramagnets at Au Surface: A root to Magneto-Molecular-Electronics

Few examples of the exploitation of molecular magnetic properties in molecular electronics are known to date. Here we propose the realization of Self assembled monolayers (SAM) of a particular stable organic radical. This radical is meant to be used as a standard molecule on which to prove the validity of a single spin reading procedure known as ESR-STM. We also discuss a range of possible applications, further than ESR-STM, of magnetic monolayers of simple purely organic magnetic molecule.Comment: This preprint is currently partially under revisio

The Diversity of Extrasolar Terrestrial Planets

Extrasolar planetary host stars are enriched in key planet-building elements. These enrichments have the potential to drastically alter the building blocks available for terrestrial planet formation. Here we report on the combination of dynamical models of late-stage terrestrial planet formation within known extrasolar planetary systems with chemical equilibrium models of the composition of solid material within the disk. This allows us to constrain the bulk elemental composition of extrasolar terrestrial planets. A wide variety of resulting planetary compositions exist, ranging from those that are essentially "Earth-like", containing metallic Fe and Mg-silicates, to those that are dominated by graphite and SiC. This implies that a diverse range of terrestrial planets are likely to exist within extrasolar planetary systems.Comment: 4 pages, 1 figure. Submitted to the proceedings of IAU symposium 265 Chemical Abundances in the Universe: Connecting First Stars to Planet

Design Solutions For Modular Satellite Architectures

The cost-effective access to space envisaged by ESA would open a wide range of new opportunities and markets, but is still many years ahead. There is still a lack of devices, circuits, systems which make possible to develop satellites, ground stations and related services at costs compatible with the budget of academic institutions and small and medium enterprises (SMEs). As soon as the development time and cost of small satellites will fall below a certain threshold (e.g. 100,000 to 500,000 €), appropriate business models will likely develop to ensure a cost-effective and pervasive access to space, and related infrastructures and services. These considerations spurred the activity described in this paper, which is aimed at: - proving the feasibility of low-cost satellites using COTS (Commercial Off The Shelf) devices. This is a new trend in the space industry, which is not yet fully exploited due to the belief that COTS devices are not reliable enough for this kind of applications; - developing a flight model of a flexible and reliable nano-satellite with less than 25,000€; - training students in the field of avionics space systems: the design here described is developed by a team including undergraduate students working towards their graduation work. The educational aspects include the development of specific new university courses; - developing expertise in the field of low-cost avionic systems, both internally (university staff) and externally (graduated students will bring their expertise in their future work activity); - gather and cluster expertise and resources available inside the university around a common high-tech project; - creating a working group composed of both University and SMEs devoted to the application of commercially available technology to space environment. The first step in this direction was the development of a small low cost nano-satellite, started in the year 2004: the name of this project was PiCPoT (Piccolo Cubo del Politecnico di Torino, Small Cube of Politecnico di Torino). The project was carried out by some departments of the Politecnico, in particular Electronics and Aerospace. The main goal of the project was to evaluate the feasibility of using COTS components in a space project in order to greatly reduce costs; the design exploited internal subsystems modularity to allow reuse and further cost reduction for future missions. Starting from the PiCPoT experience, in 2006 we began a new project called ARaMiS (Speretta et al., 2007) which is the Italian acronym for Modular Architecture for Satellites. This work describes how the architecture of the ARaMiS satellite has been obtained from the lesson learned from our former experience. Moreover we describe satellite operations, giving some details of the major subsystems. This work is composed of two parts. The first one describes the design methodology, solutions and techniques that we used to develop the PiCPoT satellite; it gives an overview of its operations, with some details of the major subsystems. Details on the specifications can also be found in (Del Corso et al., 2007; Passerone et al, 2008). The second part, indeed exploits the experience achieved during the PiCPoT development and describes a proposal for a low-cost modular architecture for satellite

The Stellar Populations of NGC 3109: Another Dwarf Irregular Galaxy with a Population II Stellar Halo

We have obtained V and I-band photometry for about 17500 stars in the field of the dwarf irregular galaxy NGC3109, located in the outskirts of the Local Group. The photometry allows us to study the stellar populations present inside and outside the disk of this galaxy. From the VI color-magnitude diagram we infer metallicities and ages for the stellar populations in the main body and in the halo of NGC3109. The stars in the disk of this galaxy have a wide variety of ages, including very young stars with approximately 10^7 yr. Our main result is to establish the presence of a halo consisting of population II stars, extending out to about 4.5 arcmin (or 1.8 kpc) above and below the plane of this galaxy. For these old stars we derive an age of > 10 Gyr and a metallicity of [Fe/H] = -1.8 +/- 0.2. We construct a deep luminosity function, obtaining an accurate distance modulus (m-M)_0 = 25.62 +/- 0.1 for this galaxy based on the I-magnitude of the red giant branch (RGB) tip and adopting E(V-I) = 0.05.Comment: Accepted for publication in the Astronomical Journal 23 pages, latex, 12 Figures (Fig 1 not available in electronic format

Keep Rollin' - Whole-Body Motion Control and Planning for Wheeled Quadrupedal Robots

We show dynamic locomotion strategies for wheeled quadrupedal robots, which combine the advantages of both walking and driving. The developed optimization framework tightly integrates the additional degrees of freedom introduced by the wheels. Our approach relies on a zero-moment point based motion optimization which continuously updates reference trajectories. The reference motions are tracked by a hierarchical whole-body controller which computes optimal generalized accelerations and contact forces by solving a sequence of prioritized tasks including the nonholonomic rolling constraints. Our approach has been tested on ANYmal, a quadrupedal robot that is fully torque-controlled including the non-steerable wheels attached to its legs. We conducted experiments on flat and inclined terrains as well as over steps, whereby we show that integrating the wheels into the motion control and planning framework results in intuitive motion trajectories, which enable more robust and dynamic locomotion compared to other wheeled-legged robots. Moreover, with a speed of 4 m/s and a reduction of the cost of transport by 83 % we prove the superiority of wheeled-legged robots compared to their legged counterparts.Comment: IEEE Robotics and Automation Letter