Additional file 2: Figure S1. of Transcriptome profiling of two maize inbreds with distinct responses to Gibberella ear rot disease to identify candidate resistance genes

Comparison between ddPCR and RNA-Seq expression profiles of selected genes. The Y-axis scale corresponds to transcripts per million (TPM) for RNA-Seq data and copies/Îźl for ddPCR. Tissue samples from the 2004 and 2006 field season were used for both gene expression quantitation methods. (PDF 288 kb

Additional file 1: Table S1. of Transcriptome profiling of two maize inbreds with distinct responses to Gibberella ear rot disease to identify candidate resistance genes

Primers used for selected candidate and reference genes in droplet digital PCR validation experiment. Table S2. Hourly temperature readings during inoculation and tissue collection time periods in 2004, 2006, and 2013. Table S3. Mapping of RNA-Seq reads to the reference genome B73 V2. Table S4. List of significant differentially expressed transcripts. Table S5. Upregulated transcripts mapping within GER resistance QTL regions. (XLSX 1156 kb

Hydroxylation of Longiborneol by a <i>Clm2</i>-Encoded CYP450 Monooxygenase to Produce Culmorin in <i>Fusarium graminearum</i>

A second structural gene required for culmorin biosynthesis in the plant pathogen <i>Fusarium graminearum</i> is described. <i>Clm2</i> encodes a regio- and stereoselective cytochrome P450 monooxygenase for C-11 of longiborneol (<b>1</b>). <i>Clm2</i> gene disruptants were grown in liquid culture and assessed for culmorin production via HPLC-evaporative light scattering detection. The analysis indicated a complete loss of culmorin (<b>2</b>) from the liquid culture of the Δ<i>Clm2</i> mutants. Culmorin production resumed in a Δ<i>Clm2</i> complementation experiment. A detailed analysis of the secondary metabolites extracted from the large-scale liquid culture of disruptant Δ<i>Clm2</i>D20 revealed five new natural products: 3-hydroxylongiborneol (<b>3</b>), 5-hydroxylongiborneol (<b>4</b>), 12-hydroxylongiborneol (<b>5</b>), 15-hydroxylongiborneol (<b>6</b>), and 11-<i>epi</i>-acetylculmorin (<b>7</b>). The structures of the new compounds were elucidated by a combination of HRMS, 1D and 2D NMR, and X-ray crystallography