Frequency (median) of CD4⁺ T cell subsets expressing different combinations of CD38, HLA-DR and CCR5 molecules in PBMC collected before vaccination (V1).

<p>Seroconversors (Sc+), Non-seroconversors (Sc−).</p><p>*p-value <0.05 comparing Sc- versus Sc+</p><p>Frequency (median) of CD4⁺ T cell subsets expressing different combinations of CD38, HLA-DR and CCR5 molecules in PBMC collected before vaccination (V1).</p

Strategy of analysis of Flow Cytometer data from one representative experiment with PBMC samples of HIV infected children.

<p>(A) Were used high Quality Parameters: (Time x PE-to check laser status; Singlets-to exclude the doublets cells; SSC x FSC- to choose lymphocytes subset; Live/Dead-to exclude the dead cells; gate SSC x CD3- to chose T lymphocytes and gate CD4 x CD8- to separate in CD4 and CD8 T subsets, then b Naïve/Memory subsets and cellular activation were evaluated in CD4 T Cells and CD8 T Cells, (B) Naïve/Memory CD4⁺ Subsets were classified through differentiated expression of three markers (CD45RA, CD27 and CCR7): 1- Naïve (CD45RA⁺CD27⁺CCR7⁺); 2- Central Memory (CD45RA⁻CD27⁺CCR7⁺); 3- Transitory Memory (CD45RA⁻CD27⁺CCR7⁻); 4- Intermediary Memory (CD45RA⁺CD27⁺CCR7⁻); 5- Effector Memory (CD45RA⁻CD27⁻CCR7⁻); 6- Effector (CD45RA⁺CD27⁻CCR7⁻); Cellular Activation were characterized through expression of CD38, HLA-DR and CCR5.</p

Triple positive CD4⁺ T cells negatively correlates with bactericidal antibody titers.

<p>CD4⁺ T_NAIVE (A) and T_EM (B) T cells with CD38⁺DR⁺CCR5⁺ phenotype correlates negatively with post-vacciantion bactericidal titers (V2). Correlations were analyzed using Sperman rank test.</p

Predominance of CD4⁺ T_INT memory subset among HIV⁺ seroconverters.

<p>Frequency and median (lines) of CD4⁺ T_Naive (A), T_CM (B), T_TM (C), T_INT (D), T_EM(E) and T_Eff (F) subpopulations in seroconverters (Sc+, closed symbols) and non-seroconverters (Sc-, open symbols) before (V1) and after (V2) vaccination.</p