Culture of airway epithelial cells from neonates sampled within 48-hours of birth

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Antenatal and delivery data of sampled neonates (n = 149) recruited to the cohort study.

<p>Antenatal and delivery data of sampled neonates (n = 149) recruited to the cohort study.</p

Cytospin of nasal sample obtained by brushing (size marker = 200 µm).

<p>Cytospin of nasal sample obtained by brushing (size marker  =  200 µm).</p

Characteristics of cell growth by passage.

<p>Characteristics of cell growth by passage.</p

Technique for harvesting nasal epithelial cells from newborn infants.

<p>Written informed consent, as outlined in the PLOS consent form, to publication of this photograph was given by the child’s legal guardian.</p

Geometric mean (95% CI) IL-8 responses of nasal AEC incubated with (A) increasing concentrations of TNF-α/IL-1β (n = 15), (B) increasing concentrations of HDM (n = 10) and (C) media, HDM (25 µg/ml) and IL-1β and TNF-α (both at 10 ng/ml) for 24 hours (n = 52).

<p>Repeated measures ANOVA and paired t-tests for statistical significance.</p