Nanofat injector: a low-cost disposable device for standardization and optimization of grafting time

Introduction: Nanofat graft improves skin quality in damage secondary to aging and scar sequelae. We present the initial results of the nanofat graft using a low-cost disposable device, proposing a standardization of its use according to the area to be treated. Methods: A prospective cohort was conducted from July 2019 to March 2020. The inclusion criterion was patients who underwent nanofat grafting for skin treatment. The exclusion criterion was the previous performance of some invasive treatment of the skin. Twenty consecutive patients who met the prerequisites were analyzed. The results were evaluated in the 6th postoperative month. The patients answered a questionnaire, classifying from 1 - very bad to 10 - excellent, changes in skin quality. Results: The twenty patients followed did not present any postoperative complications. Edema after the application was reduced between three and seven days. There was no hematoma or infection. Patients who underwent only nanofat grafting without another associated surgery could return to their activities after 24 hours. The scores reported by patients at 6 months were between 7 and 10, with a mean of 8. Conclusion: The use of the SmartneedleTM system for nanofat grafting presents patient satisfaction similar to other application methods and allows a uniform and standardized distribution of the graft according to the anatomical region and optimizing surgical tim

Forearm reconstruction with acellular dermal matrix after giant plexiform neurofibroma excision: case reports

Introduction: Giant plexiform neurinoma is a neuroectoderm and inherited disease. It is an uncommon skin tumor associated with NF1, characterized as a benign peripheral nerve sheath tumor surrounding multiple nervous fascicles. The goals of forearm coverage reconstruction are to protect the structures running to the wrist and hand and prevent scarring that leads to movement loss. Both forearm and hand play functional and social roles. Successful management of complex wounds is necessary for the overall functional rehabilitation of these patients. Case Report: A 31-year-old woman presented at the plastic surgery division with a giant plexiform neurofibroma in the right forearm. After careful resection, all anterior forearm tendons were exposed. The defect was covered with graftable Pelnac T (thickness of 3mm and sizing 12 X 24cm2), fixed with 4-0 monocryl sutures. After 10 days, the acellular dermal matrix silicone layer was removed, and a split-thickness skin meshed graft was placed. On day 7, the acellular dermal matrix showed good signs of intake. On day 17, we observed a 95% graft survival. At the 3-month follow-up, reconstruction was stable without contouring defects, the hand had full range of motion, and the patient had no problems in daily activities. Conclusions: Acellular dermal matrix appears to be a useful option in covering complex defects in the forearm, allowing for less morbidity and rapid functional recovery

Chronic Metabolic Derangement-Induced Cognitive Deficits and Neurotoxicity Are Associated with REST Inactivation

Chronic metabolic alterations may represent a risk factor for the development of cognitive impairment, dementia, or neurodegenerative diseases. Hyperglycemia and obesity are known to imprint epigenetic markers that compromise the proper expression of cell survival genes. Here, we showed that chronic hyperglycemia (60 days) induced by a single intraperitoneal injection of streptozotocin compromised cognition by reducing hippocampal ERK signaling and by inducing neurotoxicity in rats. The mechanisms appear to be linked to reduced active DNA demethylation and diminished expression of the neuroprotective transcription factor REST. The impact of the relationship between adiposity and DNA hypermethylation on REST expression was also demonstrated in peripheral blood mononuclear cells in obese children with reduced levels of blood ascorbate. The reversible nature of epigenetic modifications and the cognitive impairment reported in obese children, adolescents, and adults suggest that the correction of the anthropometry and the peripheral metabolic alterations would protect brain homeostasis and reduce the risk of developing neurodegenerative diseases.Fil: Remor, Aline Pertile. Universidade Federal de Santa Catarina; Brasil. Universidade do Oeste de Santa Catarina; BrasilFil: Da Silva, Rodrigo Augusto. Universidade Federal de Santa Catarina; Brasil. Universidade Estadual de São Paulo; BrasilFil: de Matos, Filipe José. Universidade Federal de Santa Catarina; BrasilFil: Glaser, Viviane. Universidade Federal de Santa Catarina; BrasilFil: Paula Martins, Roberta de. Universidade Federal de Santa Catarina; BrasilFil: Ghisoni, Karina. Universidade Federal de Santa Catarina; BrasilFil: Luz Scheffer, Débora da. Universidade Federal de Santa Catarina; BrasilFil: Andia, Denise Carleto. Universidade Paulista. Faculdade de Odontologia. Área de Pesquisa em Epigenética; BrasilFil: Portinho, Daniele. Universidade Estadual de São Paulo; BrasilFil: de Souza, Ana Paula. Universidade Estadual de São Paulo; BrasilFil: Oliveira, Paulo Alexandre de. Universidade Federal de Santa Catarina; BrasilFil: Prediger, Rui Daniel. Universidade Federal de Santa Catarina; BrasilFil: Torres, Alicia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; ArgentinaFil: Linhares, Rose Marie Mueller. Universidade Federal de Santa Catarina; Brasil. Hospital Infantil Joana de Gusmão; BrasilFil: Walz, Roger. Universidade Federal de Santa Catarina; BrasilFil: Ronsoni, Marcelo Fernando. Universidade Federal de Santa Catarina; BrasilFil: Hohl, Alexandre. Universidade Federal de Santa Catarina; BrasilFil: Rafacho, Alex. Universidade Federal de Santa Catarina; BrasilFil: Aguiar, Aderbal Silva. Universidade Federal de Santa Catarina; BrasilFil: de Paul, Ana Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopía Electrónica; ArgentinaFil: Latini, Alexandra. Universidade Federal de Santa Catarina; Brasil. Harvard Medical School; Estados Unido