Mung Bean nuclease mapping of RNAs 3' end

A method is described that allows an accurate mapping of 3' ends of RNAs. In this method a labeled DNA probe, containing the presumed 3' end of the RNA under analysis is allowed to anneals to the RNA itself. Mung-bean nuclease is then used to digest single strands of both RNA and DNA. Electrophoretic fractionation of "protected" undigested, labeled DNA is than performed using a sequence reaction of a known DNA as length marker. This procedure was applied to the analysis of both a polyA RNA (Interleukin 10 mRNA) and non polyA RNAs (sea urchin 18S and 26S rRNAs). This method might be potentially relevant for the evaluation of the role of posttrascriptional control of IL-10 in the pathogenesis of the immune and inflammatory mediated diseases associated to ageing. This might allow to develop new strategies to approach to the diagnosis and therapy of age related diseases

Substance use and depression. Comparative course in adolescents

Objective: To examine the relation between depression and substance use in adolescents and the concomitant courses of both disorders. Methods: Four individual interviews were administered to 85 adolescent substance users aged 14-19 years (mean 17.1 years, SD 1.4) over a 3.5 year period using the Adolescent Drug Abuse Interview (ADAD) and the Beck Depression Inventory (BDI-13). Results: No predictive effect was observed on one dimension over the other, but each dimension was predictive of its own course. A decrease in substance-use severity paralleled a decrease in depressive state. Similarly, stable substance-use rates, either at a low or a high level, tended to be associated with low or high levels of depression, respectively. However, an increase in substance use was not accompanied by an increase in depressive states. Moreover, depression varied greatly between adolescents, and according to gender and age. Conclusions: Depressive states and substance use in adolescents can vary considerably overtime, and are closely but rather synchronically related. Since most of the adolescents do not seek help for substance-related problems, substance use should be systematically assessed in adolescents presenting with a depressive stat

Activity and rotation of the X-ray emitting Kepler stars

The relation between magnetic activity and rotation in late-type stars provides fundamental information on stellar dynamos and angular momentum evolution. Rotation/activity studies found in the literature suffer from inhomogeneity in the measure of activity indexes and rotation periods. We overcome this limitation with a study of the X-ray emitting late-type main-sequence stars observed by XMM-Newton and Kepler. We measure rotation periods from photometric variability in Kepler light curves. As activity indicators, we adopt the X-ray luminosity, the number frequency of white-light flares, the amplitude of the rotational photometric modulation, and the standard deviation in the Kepler light curves. The search for X-ray flares in the light curves provided by the EXTraS (Exploring the X-ray Transient and variable Sky) FP-7 project allows us to identify simultaneous X-ray and white-light flares. A careful selection of the X-ray sources in the Kepler field yields 102 main-sequence stars with spectral types from A to M. We find rotation periods for 74 X-ray emitting main-sequence stars, 22 of which without period reported in the previous literature. In the X-ray activity/rotation relation, we see evidence for the traditional distinction of a saturated and a correlated part, the latter presenting a continuous decrease in activity towards slower rotators. For the optical activity indicators the transition is abrupt and located at a period of ~ 10 d but it can be probed only marginally with this sample which is biased towards fast rotators due to the X-ray selection. We observe 7 bona-fide X-ray flares with evidence for a white-light counterpart in simultaneous Kepler data. We derive an X-ray flare frequency of ~ 0.15 d^{-1} , consistent with the optical flare frequency obtained from the much longer Kepler time-series.Comment: Accepted for publication in A&A. 31 pages, 19 figure

The instrument control unit of the ARIEL payload: design evolution following the unit and payload subsystems SRR (system requirements review)

ARIEL (Atmospheric Remote-sensing InfraRed Large-survey) is a medium-class mission of the European Space Agency, part of the Cosmic Vision program, whose launch is foreseen by early 2029. ARIEL aims to study the composition of exoplanet atmospheres, their formation and evolution. The ARIEL’s target will be a sample of about 1000 planets observed with one or more of the following methods: transit, eclipse and phase-curve spectroscopy, at both visible and infrared wavelengths simultaneously. The scientific payload is composed by a reflective telescope having a 1m-class elliptical primary mirror, built in solid Aluminium, and two focal-plane instruments: FGS and AIRS. FGS (Fine Guidance System)1 has the double purpose, as suggested by its name, of performing photometry (0.50-0.55 µm) and low resolution spectrometry over three bands (from 0.8 to 1.95 µm) and, simultaneously, to provide data to the spacecraft AOCS (Attitude and Orbit Control System) with a cadence of 10 Hz and contributing to reach a 0.02 arcsec pointing accuracy for bright targets. AIRS (ARIEL InfraRed Spectrometer) instrument will perform IR spectrometry in two wavelength ranges: between 1.95 and 3.9 µm (with a spectral resolution R > 100) and between 3.9 and 7.8 µm with a spectral resolution R > 30. This paper provides the status of the ICU (Instrument Control Unit), an electronic box whose purpose is to command and supply power to AIRS (as well as acquire science data from its two channels) and to command and control the TCU (Telescope Control Unit)

Preliminary surface charging analysis of Ariel payload dielectrics in early transfer orbit and L2-relevant space environment

Ariel [1] is the M4 mission of the ESA’s Cosmic Vision Program 2015-2025, whose aim is to characterize by lowresolution transit spectroscopy the atmospheres of over one thousand warm and hot exoplanets orbiting nearby stars. The operational orbit of the spacecraft is baselined as a large amplitude halo orbit around the Sun-Earth L2 Lagrangian point, as it offers the possibility of long uninterrupted observations in a fairly stable radiative and thermo-mechanical environment. A direct escape injection with a single passage through the Earth radiation belts and no eclipses is foreseen. The space environment around Earth and L2 presents significant design challenges to all spacecraft, including the effects of interactions with Sun radiation and charged particles owning to the surrounding plasma environment, potentially leading to dielectrics charging and unwanted electrostatic discharge (ESD) phenomena endangering the Payload operations and its data integrity. Here, we present some preliminary simulations and analyses about the Ariel Payload dielectrics and semiconductors charging along the transfer orbit from launch to L2 include

The Ariel Instrument Control Unit: its role within the Payload and B1 Phase design

Ariel, the Atmospheric Remote-sensing Infrared Exoplanet Large-survey mission (Tinetti 2019; Puig et al. 2018; Pascale et al. 2018), has been selected in March 2018 by ESA for the fourth medium-class mission (M4) launch opportunity of the Cosmic Vision Program, with an expected lift off in late 2028. It is the first mission dedicated to measuring the chemical composition and thermal structures of the atmospheres of hundreds of transiting exoplanets, enabling planetary science far beyond the boundaries of our own Solar System. Its Payload (P/L) (Eccleston and Tinetti 2018; Eccleston et al. 2017; Middleton et al. 2019), has been designed to perform transit spectroscopy from space during primary and secondary planetary eclipses in order to achieve a large unbiased survey concerning the nature of exoplanets atmospheres and their interiors, to determine the key factors affecting the formation and evolution of planetary systems (Tinetti et al. 2017, 2018). Ariel will observe hundreds of warm and hot transiting gas giants, Neptunes and super-Earths around a wide range of host star types, targeting planets hotter than ∼ 600 K to take advantage of their well-mixed atmospheres. It will exploit primary and secondary transit spectroscopy in the 1.10 to 7.80 μm spectral range and broad-band photometry in the optical (0.50 - 0.80 μm) and Near IR (0.80 - 1.10 μm). One of the two instruments of the Ariel Payload is the Fine Guidance System (FGS), including three photometric channels (two used for guiding as well as science) between 0.5-1.1 μm plus a low resolution NIR spectrometer for 1.1-1.95 μm range. Along with FGS an IR Spectrometer (AIRS) (Amiaux et al. 2017) is foreseen, providing low-resolution spectroscopy in two IR channels: Channel 0 (CH0) for the 1.95 − 3.90 μm band and Channel 1 (CH1) for the 3.90 − 7.80 μm range. Finally, an Active Cooler System (ACS) including a Ne Joule-Thomson cooler is adopted to provide active cooling capability to the AIRS detectors working at cryogenic temperatures. AIRS is located at the intermediate focal plane of the telescope and common optical system and it hosts two HgCdTe-based hybrid IR detectors and two cold front-end electronics (CFEE) for detectors control and readout. Each CFEE is driven by a Detector Control Unit (DCU) part of AIRS but hosted within and managed by the Instrument Control Unit (ICU) of the Payload (Focardi et al. 2018). ICU is a warm unit residing into the S/C Service Module (SVM) and it is based on a cold redundant configuration involving the Power Supply Unit (PSU) and the Commanding and Data Processing Unit (CDPU) boards; both DCUs are instead cross-strapped and can be managed by the nominal or the redundant (PSU+CDPU) chain. ICU is in charge of AIRS management, collecting scientific and housekeeping (HK) telemetries from the spectrometer and HK from the telescope (temperatures readings), the P/L Optical Bench (OB) and other Subsystems (SS), thanks to a warm slave unit (TCU, Telescope Control Unit) interfaced to the ICU. Science and HK telemetries are then forwarded to the S/C, for temporary storage, before sending them to Ground. Here we describe the status of the ICU design at the end of B1 Phase, prior to the Mission Adoption Review (MAR) by ESA, with some still open architectural choices to be addressed and finalised once selected the ICU industrial Prime contractor

Serum Albumin Is Inversely Associated With Portal Vein Thrombosis in Cirrhosis

We analyzed whether serum albumin is independently associated with portal vein thrombosis (PVT) in liver cirrhosis (LC) and if a biologic plausibility exists. This study was divided into three parts. In part 1 (retrospective analysis), 753 consecutive patients with LC with ultrasound-detected PVT were retrospectively analyzed. In part 2, 112 patients with LC and 56 matched controls were entered in the cross-sectional study. In part 3, 5 patients with cirrhosis were entered in the in vivo study and 4 healthy subjects (HSs) were entered in the in vitro study to explore if albumin may affect platelet activation by modulating oxidative stress. In the 753 patients with LC, the prevalence of PVT was 16.7%; logistic analysis showed that only age (odds ratio [OR], 1.024; P = 0.012) and serum albumin (OR, -0.422; P = 0.0001) significantly predicted patients with PVT. Analyzing the 112 patients with LC and controls, soluble clusters of differentiation (CD)40-ligand (P = 0.0238), soluble Nox2-derived peptide (sNox2-dp; P < 0.0001), and urinary excretion of isoprostanes (P = 0.0078) were higher in patients with LC. In LC, albumin was correlated with sCD4OL (Spearman's rank correlation coefficient [r(s)], -0.33; P < 0.001), sNox2-dp (r(s), -0.57; P < 0.0001), and urinary excretion of isoprostanes (r(s), -0.48; P < 0.0001) levels. The in vivo study showed a progressive decrease in platelet aggregation, sNox2-dp, and urinary 8-iso prostaglandin F2 alpha-III formation 2 hours and 3 days after albumin infusion. Finally, platelet aggregation, sNox2-dp, and isoprostane formation significantly decreased in platelets from HSs incubated with scalar concentrations of albumin. Conclusion: Low serum albumin in LC is associated with PVT, suggesting that albumin could be a modulator of the hemostatic system through interference with mechanisms regulating platelet activation

Enabling planetary science across light-years. Ariel Definition Study Report

Ariel, the Atmospheric Remote-sensing Infrared Exoplanet Large-survey, was adopted as the fourth medium-class mission in ESA's Cosmic Vision programme to be launched in 2029. During its 4-year mission, Ariel will study what exoplanets are made of, how they formed and how they evolve, by surveying a diverse sample of about 1000 extrasolar planets, simultaneously in visible and infrared wavelengths. It is the first mission dedicated to measuring the chemical composition and thermal structures of hundreds of transiting exoplanets, enabling planetary science far beyond the boundaries of the Solar System. The payload consists of an off-axis Cassegrain telescope (primary mirror 1100 mm x 730 mm ellipse) and two separate instruments (FGS and AIRS) covering simultaneously 0.5-7.8 micron spectral range. The satellite is best placed into an L2 orbit to maximise the thermal stability and the field of regard. The payload module is passively cooled via a series of V-Groove radiators; the detectors for the AIRS are the only items that require active cooling via an active Ne JT cooler. The Ariel payload is developed by a consortium of more than 50 institutes from 16 ESA countries, which include the UK, France, Italy, Belgium, Poland, Spain, Austria, Denmark, Ireland, Portugal, Czech Republic, Hungary, the Netherlands, Sweden, Norway, Estonia, and a NASA contribution

Effect of angiotensin-converting enzyme inhibitor and angiotensin receptor blocker initiation on organ support-free days in patients hospitalized with COVID-19

IMPORTANCE Overactivation of the renin-angiotensin system (RAS) may contribute to poor clinical outcomes in patients with COVID-19. Objective To determine whether angiotensin-converting enzyme (ACE) inhibitor or angiotensin receptor blocker (ARB) initiation improves outcomes in patients hospitalized for COVID-19. DESIGN, SETTING, AND PARTICIPANTS In an ongoing, adaptive platform randomized clinical trial, 721 critically ill and 58 non–critically ill hospitalized adults were randomized to receive an RAS inhibitor or control between March 16, 2021, and February 25, 2022, at 69 sites in 7 countries (final follow-up on June 1, 2022). INTERVENTIONS Patients were randomized to receive open-label initiation of an ACE inhibitor (n = 257), ARB (n = 248), ARB in combination with DMX-200 (a chemokine receptor-2 inhibitor; n = 10), or no RAS inhibitor (control; n = 264) for up to 10 days. MAIN OUTCOMES AND MEASURES The primary outcome was organ support–free days, a composite of hospital survival and days alive without cardiovascular or respiratory organ support through 21 days. The primary analysis was a bayesian cumulative logistic model. Odds ratios (ORs) greater than 1 represent improved outcomes. RESULTS On February 25, 2022, enrollment was discontinued due to safety concerns. Among 679 critically ill patients with available primary outcome data, the median age was 56 years and 239 participants (35.2%) were women. Median (IQR) organ support–free days among critically ill patients was 10 (–1 to 16) in the ACE inhibitor group (n = 231), 8 (–1 to 17) in the ARB group (n = 217), and 12 (0 to 17) in the control group (n = 231) (median adjusted odds ratios of 0.77 [95% bayesian credible interval, 0.58-1.06] for improvement for ACE inhibitor and 0.76 [95% credible interval, 0.56-1.05] for ARB compared with control). The posterior probabilities that ACE inhibitors and ARBs worsened organ support–free days compared with control were 94.9% and 95.4%, respectively. Hospital survival occurred in 166 of 231 critically ill participants (71.9%) in the ACE inhibitor group, 152 of 217 (70.0%) in the ARB group, and 182 of 231 (78.8%) in the control group (posterior probabilities that ACE inhibitor and ARB worsened hospital survival compared with control were 95.3% and 98.1%, respectively). CONCLUSIONS AND RELEVANCE In this trial, among critically ill adults with COVID-19, initiation of an ACE inhibitor or ARB did not improve, and likely worsened, clinical outcomes. TRIAL REGISTRATION ClinicalTrials.gov Identifier: NCT0273570

Uterine rupture after prostaglandin analogues to induce midtrimester abortion

Although prostaglandins are largely used and considered safe drugs to induce midtrimester abortion, the literature reports several cases of uterine rupture consequent to their administration. We report the second ever-described case of uterine rupture after administration of gemeprost and sulprostone for midtrimester abortion in a 45 years-old women with scarred uterus