Mean beech mast intensity of the previous year, bank vole abundance, and human PUUV incidence in Germany 2001–2012.

<p>Bars = mean beech mast intensity of the previous year, solid line = bank vole abundance, and broken line = human PUUV incidence. Values are mean values ± one standard error from 7 Federal States. Upper bold values are beech mast intensity corresponding to bars at the bottom. (PUUV = Puumala virus; TN = trap nights).</p

Total reported annual human Puumala virus (PUUV) cases in Germany 2001–2012 (all 16 Federal States).

<p>Source: Robert Koch-Institute, SurvStat, <a href="http://www3.rki.de/SurvStat" target="_blank">http://www3.rki.de/SurvStat</a>, data status: 06/27/2014.</p

Classification of tree fructification [43].

<p>Classification of tree fructification [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0134124#pone.0134124.ref043" target="_blank">43</a>].</p

Number of notified human PUUV infections per Federal State in Germany from 2001–2012.

<p>Source: Robert Koch-Institute, SurvStat, <a href="http://www3.rki.de/SurvStat" target="_blank">http://www3.rki.de/SurvStat</a>, data status: 06/27/2014.</p><p>Number of notified human PUUV infections per Federal State in Germany from 2001–2012.</p

Additional file 3: of Occurrence and distribution of Giardia species in wild rodents in Germany

Figure S2. Bayesian phylogenetic analysis of all SSU rDNA sequence fragments. Unrooted phylogenetic tree comprised of 317 SSU rDNA sequences (277 G. microti, purple clade; 5 G. duodenalis, green clade; 32 G. muris, blue clade and 3 O. intestinalis, red clade). Reference sequences (GenBank accession numbers) of O. intestinalis, G. muris, G. duodenalis and G. microti are included (uncoloured sequence names). Posterior probabilities ≥ 0.5 are illustrated by black dots (proportionally increasing in size). Sequence names are color coded (colored ranges) according to the rodent host. Further color coding (inner to outer layer) was introduced according to locality [1 to 11: 1 (“Billerbeck”), 2 (“Gotha”), 3 (“Krahnberg”), 4 (“Schaderode”), 5 (“Jeeser”), 6 (“Saal”), 7 (“Kammerforst”), 8 (“Leinawald”), 9 (“Pahnaer Holz”), 10 (“Phönix Ost”), 11 (“Weissach”); see also Additional file 1: Figure S1 and Table 1], season (spring, summer, autumn), habitat (F = “forest”, GL= “grassland”) and year of sample collection (2011, 2012). Maximum likelihood analysis based on PhyML resulted in a similar tree (not shown). (TIFF 2598 kb

Additional file 1: of Occurrence and distribution of Giardia species in wild rodents in Germany

Figure S1. Map of Germany with study sites where wild rodents were captured and sampled. Rodents were captured at 11 study sites that were subdivided into five regions (refer to the color coding) from four German federal states. Please refer to Table 1 for further details on captured animals from each site. (TIFF 780 kb