Age distribution.

<p>(a) Black: age at diagnosis of primary melanoma (n = 149). (b) Grey: age at detection of brain metastasis (n = 193).</p

Subtypes of primary melanoma according to gender.

<p>Subtypes of primary melanoma according to gender.</p

Human melanoma lymph node metastases (entirely embedded).

<p>(A) Box plot comparison of lymphatic vessels (number of D2–40 positive vessels per core) in primary versus metastatic melanoma. * One case, * two cases. (B) Absence of D2–40 staining within metastatic tissue showing no lymphatic vessels (×30). (C) D2–40 positivity shows dilated lymphatic vessels present at the tumor/lymphatic parenchyma interface (arrow, ×150). (D) CD34 staining shows important blood micro-neovascularisation within metastases. (×50). (E) CD34-positive blood microvessels (arrowhead) associated with CD34–negative cells showing vascular mimicry (arrow, ×400). (F) and (G) Cells of vascular mimicries embedded in a PAS (F) and collagen IV (G) positive laminina (arrow, ×320)).</p

Human melanoma lymph node metastases (entirely embedded).

<p>(A) and (B) Vascular mimicries (arrow): (A) Melanoma cells with endothelial-like morphology, condensed nuclei, minimal Masson Fontana positive (inset) pigmentation (H&E, ×500), (B) mostly negative for PanMelanoma (red) (×150).</p

Location of the primary melanoma according to gender.

<p>Location of the primary melanoma according to gender.</p

Blood and lymphatic vessels per TMA core (0,28 mm²) in primary and metastatic melanoma.

<p>± Mean ± SD.</p

Comparison of Breslow tumour thickness between males and females.

<p>Comparison of Breslow tumour thickness between males and females.</p

Overview of melanoma xenograft experiments used for illustrating melanoma cells participation in neovascularisation.

<p>Overview of melanoma xenograft experiments used for illustrating melanoma cells participation in neovascularisation.</p

Comparison of T stages between males and females.

<p>Comparison of T stages between males and females.</p

Subtypes of primary melanoma according to the presence or absence of lymph node metastasis.

<p>Subtypes of primary melanoma according to the presence or absence of lymph node metastasis.</p