2023 06 02 Total MAPT Cerebellum 10 47

Detection of total-MAPT RNA species by digital droplet PCR in cerebellum samples of non-demented individuals and Alzheimer's disease patients. </p

2023_02_08 Cerebellum 12-39

Detection of intron 3 and intron 12 retaining MAPT species by digital droplet PCR in cerebellum samples of non-demented individuals and Alzheimer's disease patients. Each retention was detected in a different fluorophore. There are species that retain both introns TIR3+12-MAPT (Fam +, Hex +) which would be translated into DW-Tau (double intron retention), species that retain only intron 3 TIR3-MAPT (Fam +, Hex -) which would be translated into NW-Tau (Nt truncation) or species that retain only intron 12 TIR12-MAPT (Fam -, Hex +) which would be translated into CW-Tau (Ct truncation).</p

Analysis of ddPCR results csv files

Data collection of the detection of intron 3 and intron 12 retaining MAPT species by digital droplet PCR in frontal lateral cortex, hippocampal and cerebellum samples of non-demented individuals and Alzheimer's disease patients. Each retention was detected in a different fluorophore. There are species that retain both introns TIR3+12-MAPT (Fam +, Hex +) which would be translated into DW-Tau (double intron retention), species that retain only intron 3 TIR3-MAPT (Fam +, Hex -) which would be translated into NW-Tau (Nt truncation) or species that retain only intron 12 TIR12-MAPT (Fam -, Hex +) which would be translated into CW-Tau (Ct truncation). Additionally Tubulin-beta and total-MAPT levels were measured by ddPCR to using SYBRGeen for normalyzation purposes.</p

2023 06 05 Hippocapus total MAPT 11 43

Detection of total MAPT species by digital droplet PCR in hippocampal samples of non-demented individuals and Alzheimer's disease patients</p

2021 06 02 total-MAPT cortex cerebellum 13 52

Detection of intron total MAPT species by digital droplet PCR in frontal lateral cortex and cerebellum samples of non-demented individuals and Alzheimer's disease patients.</p

2023 06 16 TubB Hippocampus 11 54

Detection of tubulin-beta RNA species by digital droplet PCR in hippocampal samples of non-demented individuals and Alzheimer's disease patients.</p

Analysis of data and statistics

Data analysis of the detection of intron 3 and intron 12 retaining MAPT species by digital droplet PCR in frontal lateral cortex, hippocampal and cerebellum samples of non-demented individuals and Alzheimer's disease patients. Each retention was detected in a different fluorophore. There are species that retain both introns TIR3+12-MAPT (Fam +, Hex +) which would be translated into DW-Tau (double intron retention), species that retain only intron 3 TIR3-MAPT (Fam +, Hex -) which would be translated into NW-Tau (Nt truncation) or species that retain only intron 12 TIR12-MAPT (Fam -, Hex +) which would be translated into CW-Tau (Ct truncation). Additionally Tubulin-beta and total-MAPT levels were measured by ddPCR to perform normalization.</p

23 02 09 cortex hippocampus TIR12 TIR3 08 48

Detection of intron 3 and intron 12 retaining MAPT species by digital droplet PCR in frontal lateral cortex and hippocampal samples of non-demented individuals and Alzheimer's disease patients. Each retention was detected in a different fluorophore. There are species that retain both introns TIR3+12-MAPT (Fam +, Hex +) which would be translated into DW-Tau (double intron retention), species that retain only intron 3 TIR3-MAPT (Fam +, Hex -) which would be translated into NW-Tau (Nt truncation) or species that retain only intron 12 TIR12-MAPT (Fam -, Hex +) which would be translated into CW-Tau (Ct truncation).</p

2023 TubB Cerebellum 10 31

Detection of tubulin-beta RNA species by digital droplet PCR in cerebellum samples of non-demented individuals and Alzheimer's disease patients.</p

2023 06 19 TubB frontal lateral cortex and hippocampus 10 28

Detection of Tubulin-beta RNA species by digital droplet PCR in frontal lateral cortex and hippocampal samples of non-demented individuals and Alzheimer's disease patients.</p