Mapping the Beta-Sheet Structure of the Yeast Prion Sup35 through Creation of Targeted Mutant Forms

Proteins with an aggregated form rich in beta-sheet structure are known as amyloids, of which a subset are infectious. These infectious proteins are known as prions and cause diseases including bovine spongiform encephalopathy (“Mad Cow” disease). Several prions have been identified in the baker’s yeast, Saccharomyces cerevisiae. One of the most well-studied yeast prions is the protein Sup35. To understand the fine protein structure of Sup35 better, we used PCR-based mutagenesis to introduce a lysine residue (a charged amino acid) at five defined places in the protein sequence of Sup35. We describe our process for creating these mutant versions and the results of DNA sequencing of each mutant version. The next step will be to assess prion formation and stability of clones with the correct sequences. Understanding the behavior of yeast prions has proven helpful in understanding human amyloid diseases and further studies on these yeast prions, including Sup35, will expand our knowledge further

Cohomology and Support Varieties for Lie Superalgebras II

In \cite{BKN} the authors initiated a study of the representation theory of classical Lie superalgebras via a cohomological approach. Detecting subalgebras were constructed and a theory of support varieties was developed. The dimension of a detecting subalgebra coincides with the defect of the Lie superalgebra and the dimension of the support variety for a simple supermodule was conjectured to equal the atypicality of the supermodule. In this paper the authors compute the support varieties for Kac supermodules for Type I Lie superalgebras and the simple supermodules for $\mathfrak{gl}(m|n)$. The latter result verifies our earlier conjecture for $\mathfrak{gl}(m|n)$. In our investigation we also delineate several of the major differences between Type I versus Type II classical Lie superalgebras. Finally, the connection between atypicality, defect and superdimension is made more precise by using the theory of support varieties and representations of Clifford superalgebras.Comment: 28 pages, the proof of Proposition 4.5.1 was corrected, several other small errors were fixe

Global Hot Gas in and around the Galaxy

The hot interstellar medium traces the stellar feedback and its role in regulating the eco-system of the Galaxy. I review recent progress in understanding the medium, based largely on X-ray absorption line spectroscopy, complemented by X-ray emission and far-UV OVI absorption measurements. These observations enable us for the first time to characterize the global spatial, thermal, chemical, and kinematic properties of the medium. The results are generally consistent with what have been inferred from X-ray imaging of nearby galaxies similar to the Galaxy. It is clear that diffuse soft X-ray emitting/absorbing gas with a characteristic temperature of $\sim 10^6$ K resides primarily in and around the Galactic disk and bulge. In the solar neighborhood, for example, this gas has a characteristic vertical scale height of $\sim 1$ kpc. This conclusion does not exclude the presence of a larger-scale, probably much hotter, and lower density circum-Galactic hot medium, which is required to explain observations of various high-velocity clouds. This hot medium may be a natural product of the stellar feedback in the context of the galaxy formation and evolution.Comment: 11 pages, invited talk in the workshop "The Local Bubble and Beyond II

No confinement without Coulomb confinement

We compare the physical potential $V_D(R)$ of an external quark-antiquark pair in the representation $D$ of SU(N), to the color-Coulomb potential $V_{\rm coul}(R)$ which is the instantaneous part of the 44-component of the gluon propagator in Coulomb gauge, D_{44}(\vx,t) = V_{\rm coul}(|\vx|) \delta(t) + (non-instantaneous). We show that if $V_D(R)$ is confining, $\lim_{R \to \infty}V_D(R) = + \infty$, then the inequality $V_D(R) \leq - C_D V_{\rm coul}(R)$ holds asymptotically at large $R$, where $C_D > 0$ is the Casimir in the representation $D$. This implies that $- V_{\rm coul}(R)$ is also confining.Comment: 9 page