Transcriptional response of <i>R. rickettsii</i> exposed to 4°C for 24 hrs.

a<p>locus_tags for <i>R. rickettsii</i> Sheila Smith (CP000848) and Iowa (CP000766).</p>b<p>Comparison of gene expression levels from R. rickettsii grown for three days at 34°C and shifted to 4°C for 24 hrs to R. rickettsii grown for three days at 34°C.</p>c<p>No anotation in <i>R. rickettsii</i> Sheila Smith (CP000848).</p

Scatter plots of fold changes.

<p>A) 34°C vs 25°C. B) 34°C vs 4°C. The solid line indicates an equivalence between the two conditions, while the small dashed line indicates a two fold difference and the long-short dashed line indicates a three fold difference.</p

Transcriptional response to limiting iron conditions.

a<p>locus_tags for R. rickettsii Sheila Smith (CP000848) and Iowa (CP000766).</p>b<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 34°C and then incubated with 200 µM of deferozamine mesylate for 24 hrs to <i>R. rickettsii</i> grown for three days at 34°C.</p>c<p>Gene fragment or predicted gene less than 10 Kda.</p>d<p>No anotation in <i>R. rickettsii</i> Iowa (CP000766).</p>e<p>No anotation in <i>R. rickettsii</i> Sheila Smith (CP000848).</p

Confirmation and comparison of responses after 2 and 24 hrs at 4°C.

a<p>locus_tags for <i>R. rickettsii</i> Sheila Smith (CP000848) and Iowa (CP000766).</p>b<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 34°C and shifted to 4°C for 2 hrs to <i>R. rickettsii</i> grown for three days at 34°C.</p>c<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 34°C and shifted to 4°C for 24 hrs to <i>R. rickettsii</i> grown for three days at 34°C.</p>d<p>Gene fragments or predicted genes less than 10 kda.</p>e<p>No anotation in <i>R. rickettsii</i> Sheila Smith (CP000848).</p

Genes that differentially respond early or late after shift to 4°C.

a<p>locus_tags for <i>R. rickettsii</i> Sheila Smith (CP000848) and Iowa (CP000766).</p>b<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 34°C and shifted to 4°C for 2 hrs to <i>R. rickettsii</i> grown for three days at 34°C.</p>c<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 34°C and shifted to 4°C for 24 hrs to <i>R. rickettsii</i> grown for three days at 34°C.</p>d<p>Gene fragments or predicted genes less than 10 kda.</p>e<p>No anotation in <i>R. rickettsii</i> Sheila Smith (CP000848).</p

Transcriptional differences between Vero and ISE6 cells.

a<p>locus_tag for <i>R. rickettsii</i> Sheila Smith (CP000848) and Iowa (CP000766).</p>b<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 37°C in Vero cells to <i>R. rickettsii</i> grown for eight days at 22°C in ISE6 cells.</p>c<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 37°C in Vero cell to <i>R. rickettsii</i> grown for three days at 37°C in ISE6 cells.</p>d<p>Gene fragments or predicted genes less than 10 Kda.</p

Transcriptional response of <i>R. rickettsii</i> shifted from 34°C to 4°C to 34°C.

a<p>locus_tags for <i>R. rickettsii</i> Sheila Smith (CP000848) and Iowa (CP000766).</p>b<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 34°C and shifted to 4°C for 2 hrs to <i>R. rickettsii</i> grown for three days at 34°C.</p>c<p>Comparison of gene expression levels from <i>R. rickettsii</i> grown for three days at 34°C shifted to 4°C for 2 hrs and then back to 34°C for 2 hrs to <i>R. rickettsii</i> grown for three days at 34°C and shifted to 4°C for 2 hrs.</p>d<p>No anotation in <i>R. rickettsii</i> Sheila Smith (CP000848).</p

Growth kinetics of <i>R. rickettsii</i> under different experimental conditions.

<p>A) Growth of <i>R. rickettsii</i> in Vero cells incubated at either 34°C or 25°C. B) Growth of <i>R. rickettsii</i> at 37°C in ISE6 and Vero cells. C) Growth of <i>R. rickettsii</i> in ISE6 cells at 22°C.</p

<i>S</i>. <i>aureus</i> skin infection causes changes in transcripts encoding molecules involved in Th lymphocyte differentiation.

<p>The networks/functional analyses were generated through the use of QIAGEN’s Ingenuity Pathway Analysis (IPA, QIAGEN Redwood City, <a href="http://www.qiagen.com/ingenuity" target="_blank">www.qiagen.com/ingenuity</a>). The heat map representing changes in transcript levels of molecules on day 1, 3, 6, 10 and 14 during 14-day course of infection, was created using Adobe Illustrator CS5.1 software. Empty symbols indicate molecules not evaluated by the RT² Profiler PCR Array.</p

TaqMan real-time PCR verification of changes in <i>S</i>. <i>aureus</i> transcript levels.

<p>Results are expressed as an average relative fold change in bacterial transcripts from three abscesses excised 24 h post infection and compared to the level of bacterial transcript expression in the inoculum. The relative quantification of <i>S</i>. <i>aureus</i> transcript was determined by the change in expression of target transcripts relative to that of <i>gyrB</i>. *Also known as <i>lukA</i>.</p