Crotalus oreganus concolor (Viperidae; Crotalinae): A case of envenomation with venom analysis from the envenomating snake and a diagnostic conundrum of myo-neurological symptoms

Background:Crotalus oreganus concolor is a smaller species of North American rattlesnake indigenous to a confined middle region of the western United States. Reports of envenomation to humans are quite rare, and studies regarding the toxicity and pharmacological actions of C. o. concolor venom have shown the presence of low molecular weight myotoxins with myotoxic effects, and low metalloproteinase activity. Method: A case that occurred in a remote location following the bite of a captive C. o. concolor is presented. Case: The bite was sustained to the right thumb while removing the snake from a drawer-type housing unit and it resulted in a single fang puncture. Immediately, there was tingling in the bitten thumb followed by extreme tingling in the lips that progressed to the toes, and tightening of the face, forehead, and chest. Ambulance ground transport to a medical helicopter airlift site was initiated. While in transport early on the patient experienced a cascade of symptoms beginning with blurry vision, total body paresthesia, dyspnea and transient diaphragmatic paralysis followed by three waves of spastic muscle paresis of the hands and feet. These symptoms were transient and had resolved spontaneously prior to arrival at the hospital three hours post bite. Patient laboratory values and coagulation parameters remained within normal limits, except for a mild increase in D-dimer, elevated creatine kinase, and reduced total calcium. Local envenomation at the bite site was minimal, but numbness of the thumb persisted for longer than a week. For various reasons antivenom was not administered at any stage and symptoms were successfully managed symptomatically. At 24 hours, and following discharge, the patient was experiencing total body weakness, but able to walk slowly without assistance. He continued to experience myalgias in the right arm and overall generalized weakness for several days. Several weeks later sloughing of skin around the bite site was observed, but other local symptoms, other than numbness of the thumb, had resolved completely. Venom from the offending snake was collected and venom analysis performed revealing the presence of very high levels of myotoxins, small peptides that induce rapid tetanic muscle contractions in mice, and several serine proteinases often associated with coagulopathies. Discussion/Conclusion: Causes of the patient’s transient myo-neurological symptoms were confounding diagnostically with respect to those that were potentially venom-induced versus those that may have been stress-induced physiological responses

Crotalus oreganus concolor: Envenomation Case with Venom Analysis and a Diagnostic Conundrum of Myoneurologic Symptoms

This is an accepted manuscript of an article published by Elsevier in Wilderness & Environmental Medicine on 12/03/2020, available online: https://doi.org/10.1016/j.wem.2019.12.007 The accepted version of the publication may differ from the final published version.A case of midget-faded rattlesnake (Crotalus oreganus concolor) envenomation of an adult male professional herpetologist occurred in a rural setting and resulted in an array of venom induced myoneurologic symptoms. The patient experienced blurry vision, total body paresthesia, dyspnea, chest tightness, and waves of spastic muscle movements of the hands and feet that resembled tetany. It was not apparent whether these symptoms were potentially venom induced or were related to stress-induced physiologic responses. Local envenomation effects were minimal, and coagulation parameters remained within normal limits. Antivenom was not administered per patient concerns related to a history of acute allergic reactions to antivenom. Venom was collected from the Crotalus oreganus concolor responsible for the bite, and analysis revealed the presence of high levels of myotoxins (SR calcium pump antagonists) and concolor toxin, a presynaptic neurotoxin that can have myotoxic effects and cause respiratory paralysis; several serine proteinases associated with coagulopathies were also present in the venom profile.Published versio

Determination of the in vivo antigen-antibody affinity constant from the redistribution of desipramine in rats following administration of a desipramine-specific monoclonal antibody

Quantitative expressions have been derived to determine the affinity constant for the in vivo interaction of an antigen with its elicited monoclonal antibody by analysing the redistribution of antigen following antibody administration. Using this method, the intrinsic binding constant for the interaction of subtoxic doses of DMI in rats with anti-TCA was found to be about two orders of magnitude less than the value obtained in vitro. The disparity is probably due to the presence of endogenous ligands for the antibody