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    Evaluation of interface quality in organ-cultured lamellar corneal transplants

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    Background: With increasing numbers of lamellar keratoplasties, eye banks are challenged to deliver precut lamellar donor tissue. In Europe, the most common technique of corneal storage is organ culture which requires a deswelling process before surgical processing. The aim of this study was to investigate the influence of different deswelling times on the cutting plane quality after microkeratome-assisted lamellar dissection. Methods: Eight paired donor corneas (16 specimens) not suitable for transplantation were organ cultured under standard conditions at the Eye Bank of the Ludwig-Maximilians UniversitÀt, Munich, Germany. Pairs of corneal buttons were analyzed during the deswelling process in dextrane-containing medium. While one cornea was cut at an early time point during the deswelling process and put back into deswelling medium thereafter, the partner cornea was completely deswollen and dissected after 72 hours. Specimens were then further processed for scanning electron microscopy. Surface quality was assessed both digitally using Scanning Probe Imaging Processing software, and manually by three blinded graders. Results: The corneal buttons processed at the beginning of the deswelling process had a smoother surface when compared to the partner cornea that was cut at the end of the deswelling process. In our setting, no relevant difference was detectable between manual and automated microkeratome dissection. Conclusion: For lamellar keratoplasty, organ-cultured corneas should be processed at an early stage during the deswelling process. We interpret the smoother dissection plane during early deswelling as a result of mechanical properties in a highly hydrated cornea

    The Wolf effect and the Redshift of Quasars

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    We consider a simple model, based on currently accepted models for active galactic nuclei, for a quasi-stellar object (QSO or ``quasar'') and examine the influence that correlation- induced spectral changes (``The Wolf Effect'') may have upon the redshifts of the optical emission lines.Comment: 13 pages, 3 figures. To be published in J. European Optical Soc. A: Pure and Applied Optic

    Cosmological Implications of the Uncertainty in H– Destruction Rate Coefficients

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    In primordial gas, molecular hydrogen forms primarily through associative detachment of H- and H, thereby destroying the H-. The H- anion can also be destroyed by a number of other reactions, most notably by mutual neutralization with protons. However, neither the associative detachment nor the mutual neutralization rate coefficients are well determined: both may be uncertain by as much as an order of magnitude. This introduces a corresponding uncertainty into the H2 formation rate, which may have cosmological implications. Here we examine the effect that these uncertainties have on the formation of H2 and the cooling of protogalactic gas in a variety of situations. We show that the effect is particularly large for protogalaxies forming in previously ionized regions, affecting our predictions of whether or not a given protogalaxy can cool and condense within a Hubble time, and altering the strength of the ultraviolet background that is required to prevent collapse

    Intravitreal bevacizumab for the treatment of macular oedema secondary to branch retinal vein occlusion

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    Purpose: To evaluate the effect of intravitreal bevacizumab (Avastin) injections on visual acuity (VA) and foveal retinal thickness in patients with macular oedema secondary to branch retinal vein occlusion.Methods: A prospective, non-comparative, consecutive, interventional case series of 34 patients. Patients received repeated intravitreal injections of 1.25 mg bevacizumab. Main outcome measures were VA (Snellen charts and ETDRS) and retinal thickness (optical coherence tomography measurements) in a follow-up period of 6 months.Results: Patients presented at a mean age of 69 years (range 44--86). Mean duration of symptoms was 40 weeks (range 1--300). Mean (SD) VA at baseline was 0.79 (0.39) logMAR, improving to 0.51 (0.34) logMAR at 6 months (p = 0.009). Mean number of letters on the ETDRS chart at baseline was 45.3 (19.0), improving to 60.6 (19.9) at 6 months (p = 0.003). Mean (SD) retinal thickness at baseline was 474 (120) \textgreekmm, declining to 316 (41) \textgreekmm at 6 months.Conclusion: Intravitreal injection of 1.25 mg bevacizumb appears to be an effective treatment option for branch retinal vein occlusion

    Chiral criticality in doped Mn1−y_{1-y}Fey_ySi compounds

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    The critical spin fluctuations in doped compounds Mn1−y_{1-y}Fey_ySi have been studied by means of ac-susceptibility measurements, polarized neutron small angle scattering and spin echo spectroscopy. It is shown that these compounds undergo the transition from the paramagnetic to helimagnetic phase through continuous, yet well distinguishable crossovers: (i) from paramagnetic to partially chiral, (ii) from partially chiral to highly chiral fluctuating state. The crossover points are identified on the basis of combined analysis of the temperature dependence of ac-susceptibility and polarized SANS data. The whole transition is marked by two inflection point of the temperature dependence of ac-susceptibility: the upper one corresponds to the crossover to partially chiral state at T∗T^*, where the inverse correlation length Îș≈2k\kappa \approx 2 k, the lower one corresponds to the transition to the spin helix structure. The intermediate crossover to the highly chiral phase is observed at the inflection point TkT_k of the first derivative of ac-susceptibility, where Îș≈k\kappa \approx k. The temperature crossovers to the highly chiral fluctuating state is associated with the enhancing influence of the Dzyaloshinskii-Moria interaction close to TcT_c.Comment: 5 pages, 5 figures, 1 table, 13 cite
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