Double mutations in eIF4E and eIFiso4E confer recessive resistance to Chilli veinal mottle virus in pepper

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinai mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an eIF4E mutation (pvr1 2 ) and C. annuum 'Perennial' containing an eIFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All F1 plants showed resistance, and F2 individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five F2 and 329 F3 plants of 17 families were genotyped with pvr1 2 and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both pvr1 2 and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in eIF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of F2 plants revealed that all plants containing homozygous genotypes of both pvr1 2 and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of eIF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper. © 2009 The Korean Society for Molecular and Cellular Biology and Springer Netherlands

The PHEMU09 catalogue and astrometric results of the observations of the mutual occultations and eclipses of the Galilean satellites of Jupiter made in 2009

Context. In 2009, the Sun and the Earth passed through the equatorial plane of Jupiter and therefore the orbital planes of its main satellites. It was the equinox on Jupiter. This occurrence made mutual occultations and eclipses between the satellites possible. Experience has shown that the observations of such events provide accurate astrometric data able to bring new information on the dynamics of the Galilean satellites. Observations are made under the form of photometric measurements, but need to be made through the organization of a worldwide observation campaign maximizing the number and the quality of the data obtained. Aims. This work focuses on processing the complete database of photometric observations of the mutual occultations and eclipses of the Galilean satellites of Jupiter made during the international campaign in 2009. The final goal is to derive new accurate astrometric data. Methods. We used an accurate photometric model of mutual events adequate with the accuracy of the observation. Our original method was applied to derive astrometric data from photometric observations of mutual occultations and eclipses of the Galilean satellites of Jupiter. Results. We processed the 457 lightcurves obtained during the international campaign of photometric observations of the Galilean satellites of Jupiter in 2009. Compared with the theory, for successful observations, the r.m.s. of O-C residuals are equal to 45.8 mas and 81.1 mas in right ascension and declination, respectively; the mean O-C residuals are equal to -2 mas and -9 mas in right ascension and declination, respectively, for mutual occultations; and -6 mas and +1 mas in right ascension and declination, respectively, for mutual eclipses. © ESO, 2014