Antibiotic Susceptibility Profile of respiratory pathogens obtained at tertiary care hospital from western Nepal

The prevalence and drug resistance of the respiratory pathogens is increasing gradually in Nepal. However, their detail study is rare in the western region of Nepal. Hence, this study was carried out to know the incidence and antibiotic susceptibility profile of the respiratory pathogens obtained at a tertiary care center located at Pokhara. 139 pathogens were isolated from 460 clinical samples included. Significant pathogens were Gram-negative bacteria 94 (67.62%), followed by 28 (20.15%) Candida, and Gram-positive isolates 17 (12.23%). The growth rate was significantly higher for sputum samples in comparison to throat swabs. Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter were significant Gram-negative isolates while Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus pyogenes were Gram-positive pathogens. Sensitivity rate was higher for colistin and imipenem among Gram-negative isolates while lower sensitivity was for cefepime. Vancomycin was effective against all tested Gram-positive isolates while erythromycin and ciprofloxacin were less effective

Heading towards the Safer Highways: an assessment of the Avahan prevention programme among long distance truck drivers in India

<p>Abstract</p> <p>Background</p> <p>Using data from two rounds of a cross-sectional, national-level survey of long-distance truck drivers, this paper examines the extent and trend of sexual risk behavior, prevalence of STI/HIV, and the linkage between exposure to HIV prevention programs and safe sex behavior.</p> <p>Methods</p> <p>Following the time location cluster sampling approach, major transshipment locations covering the bulk of India’s transport volume along four routes, North-East (NE), North-South (NS), North-West (NW) and South-East (SE) were surveyed. First round of the survey was conducted in 2007 (sample size 2066) whereas the second round was undertaken in 2009-2010 (sample size 2085). Long distance truck drivers were interviewed about their sexual behaviors, condom use practices, exposure to different HIV prevention interventions, and tested for HIV, reactive syphilis serology, <it>Neiserria gonorrhoeae</it> and <it>Chlamydia trachomatis.</it> The key variable of this evaluation study - exposure to HIV prevention interventions was divided into three categories - no exposure, less intensive exposure and intensive exposure. Data were analyzed using multiple logistic regression methods to understand the relationship between risk behavior and exposure to intervention and between program exposure and condom use.</p> <p>Results</p> <p>The proportion of truckers exposed to HIV prevention interventions has increased over time with much significant increase in the intensive exposure across all the four routes (NE: from 14.9% to 28%, P < 0.01; NS: from 20.9% to 38.1%; NW: 11.5% to 39.5%, P < 0.01; SE: 4.7% to 9.7%, P <0.05). Overall, the consistent condom use in sex with non-regular female partners too has increased over the time (paid female partners: from 67.1% to 73.2%, P <0.05; non-paid female partners: from 17.9% to 37.1%, P <0.05). At the aggregate level, the proportion tested HIV positive has declined from 3.2% to 2.5% in (p<0.10) and proportion tested positive for Syphilis too has reduced from 3.2% to 1.7% (p<0.05). Truckers who had sex with paid female partners (men at risk) were significantly more likely to get exposed to intensive program (aOR: 2.6, 95%CI 1.9-3.4) as compared to those who did not have sex with paid partners. Truckers who had sex with paid partners and exposed to intervention program were more likely to use condoms consistently (aOR: 2.1, 95% CI 1.2-3.7). The consistent condom use among respondents who travel through states with targeted interventions towards female sex workers was higher than those who travel through states with less intensive program among FSWs.</p> <p>Conclusions</p> <p>These evaluation study results highlight the ability of intensive program to reach truckers who have sex outside marriage with HIV prevention interventions and promote safe sex behaviors among them. Truckers who practice safe sex behaviors with an exposure to intensive program are less likely to suffer from STIs and HIV, which has implications for HIV prevention program with truckers’ population in India and elsewhere. The simultaneous targeted interventions among female sex workers appeared to have contributed to safe sexual practices among truckers.</p

Evaluation of pro-vitamin A enriched maize hybrids for fighting hidden hunger in Nepal

Prevailing vitamin A deficiency is a malnutrition repercussing retarded growth, weak immune system and night-blindness in human beings. Pro-vitamin A enriched maize hybrids could be a strategy for combating vitamin A deficiency, mostly prevailing in children and women of Nepal. With the objective to investigate superior pro-vitamin A enriched ‘bio-fortified’ maize cultivars, twice replicated experiments were laid out in α-lattice design over two consecutive growing seasons of 2019 and 2019/20 at the National Maize Research Program (NMRP), Rampur, Chitwan, Nepal. The results revealed that the difference among tested hybrids was glaring for all agro-morphological, yield, and yield components traits. Among the evaluated traits, days to 50% anthesis and silking, plant and ear height, numbers of kernel rows per cob, grains per row, and grain yield varied significantly among the tested maize hybrids. Effect of planting season was significant for grain yield where winter maize produced 32% higher grain yield than spring maize. HPO16-2, HPO49-3, HPO49-5, and HPO49-2 were the 38-61% high yielding ‘bio-fortified’ maize genotypes than normal hybrid check. Therefore, these hybrids might be the potential higher-yielding future pro-vitamin A enriched maize hybrids to resolve food insecurity, malnutrition, trade deficit on maize grains and specially to combat vitamin A deficiency in Nepal

Development and testing the feasibility of a sports-based mental health promotion intervention in Nepal: a protocol for a pilot cluster-randomised controlled trial

Background: Mental wellbeing encompasses life satisfaction, social connectedness, agency and resilience. In adolescence, mental wellbeing reduces sexual health risk behaviours, substance use and violence; improves educational outcomes; and protects mental health in adulthood. Mental health promotion seeks to improve mental wellbeing and can include activities to engage participants in sport. However, few high-quality trials of mental health promotion interventions have been conducted with adolescents, especially in low- and middle-income countries. We sought to address this gap by testing SMART (Sports-based Mental heAlth pRomotion for adolescenTs) in a pilot cluster-randomised controlled trial (cRCT) in Bardiya, Nepal. // Methods: The objectives of the trial are to assess the acceptability and feasibility of SMART, test trial procedures, explore outcome distributions in intervention and control clusters and calculate the total annual cost of the intervention and unit cost per adolescent. The trial design is a parallel-group, two-arm superiority pilot cRCT with a 1:1 allocation ratio and two cross-sectional census surveys with adolescents aged 12–19, one pre-intervention (baseline) and one post-intervention (endline). The study area is four communities of approximately 1000 population (166 adolescents per community). Each community represents one cluster. SMART comprises twice weekly football, martial arts and dance coaching, open to all adolescents in the community, led by local sports coaches who have received psychosocial training. Sports melas (festivals) and theatre performances will raise community awareness about SMART, mental health and the benefits of sport. Adolescents in control clusters will participate in sport as usual. In baseline and endline surveys, we will measure mental wellbeing, self-esteem, self-efficacy, emotion regulation, social support, depression, anxiety and functional impairment. Using observation checklists, unstructured observation and attendance registers from coaching sessions, and minutes of meetings between coaches and supervisors, we will assess intervention fidelity, exposure and reach. In focus group discussions and interviews with coaches, teachers, caregivers and adolescents, we will explore intervention acceptability and mechanisms of change. Intervention costs will be captured from monthly project accounts, timesheets and discussions with staff members. // Discussion: Findings will identify elements of the intervention and trial procedures requiring revision prior to a full cRCT to evaluate the effectiveness of SMART

Impact of adoption of heat-stress tolerant maize hybrid on yield and profitability: Evidence from Terai region of Nepal

Abiotic stresses (drought, heat) are one of the major impediments to enhancing the maize productivity of marginal farmers in the facet of climate change. The present study attempts to investigate the impact of heat-tolerant maize hybrid on yield and income in the Terai region of Nepal. This study uses cross-sectional farm household-level data collected in August 2021 from a randomly selected sample of 404 rural households. We used a doubly robust inverse probability weighted regression adjustment method to obtain reliable impact estimates. Adoption of heat-tolerant hybrid increases yields by 16% and income by 44% in the spring season (a stress condition). Overall, yield increases by 12%, net income by 31%, saving of 40% in seed costs, and per capita food expenditure increases by 8.50%. Hence a conducive environment must be created for scaling up heat-tolerant maize varieties to increase productivity, minimize risk, and transform of the maize sector

Genetic insights on single cross maize hybrid and its importance on maize self-sufficiency in Nepal

When the world's population rises, total crop production worldwide is not meeting rising food demand. Focus on developing high yielding single cross hybrids of maize that are resource-efficient under diverse soil and climatic conditions is utmost for countries like Nepal. With the aim of exploring genetic reasons for higher hybrid vigor of single cross hybrid of maize, global genetic importance and addressing the emerging issue of needed higher productivity in Nepal to achieve maize self-sufficiency status, comprehensive review work was performed. Research findings explored that; the Heterosis hypothesis has been widely exploited in crop breeding, resulting in a large increase in yield. The ability analysis is of special importance in cross-pollinated crops like maize as it helps in identifying potential parents that can be used for producing hybrids and synthetics. New molecular tools and techniques can complement traditional methods to allow breeders to tackle priority research areas efficiently. Nepal’s reliance on imported hybrid maize seed and grain increased each year as competitive hybrid cultivars became unavailable within the country. Recently, higher yield gap due to lower productivity, being the major concern in Nepal, single cross hybrids are intervention point. For the countries like Nepal, where achieving higher yield of maize to address self-sufficiency is the nationally prioritized issue, single cross hybrids of maize could be the best way to come up

Production and Trade Scenario of Major Underutilized Crops of Nepal

In Nepal, underutilized crops are the crop species which have high potential for food, nutrition, dietary and culinary diversification but are less prioritized in formal research, education, extension and development. Analysis of the trend of production and trade helps to formulate and prioritize governing policies for the development of the underutilized crops in Nepal. Analyzing this need, This study aims to reveal the production and trade scenario of major underutilized crops of Nepal; mainly Finger millet, Buckwheat and Barley over the years. Secondary data collected from Food and Agriculture Organization Corporate Statistical Database (FAOSTAT), Ministry of Agriculture and Livestock Development (MoALD), Department of Customs and relevant articles were used for the study and to analyze the ongoing trend, simple linear regression model was used. Collected and analyzed data revealed that, production area of millet is decreasing. However, the production quantity is increasing mainly because of the increased productivity. Though the production is increasing, the import of millet is also high. Increased consumption of local liquor products made from millet followed by diversified food product consumption have seems to conduced the increased demand. The production area of buckwheat found steady over a decade, however, the production and productivity is increasing. The production area and productivity of barley is decreasing while the import of barley is increasing from 2013/14 with the sudden fall in 2019/20. The sudden decrease in import in 2019/20 was due to trade restriction due to Covid situation. But now the demand of these underutilized crops is increasing year after year, and this will continue in forthcoming years also, mainly because of raise in awareness about the health and nutritional benefits. To reduce the ballooning trade deficit of these crops in near future, along with productivity, effective area expansion policies and programs should be formulated and implemented from all three tiers of government

Bio-fortified maize: Cornerstone in plant breeding to combat hidden hunger in developing countries

Malnutrition has been one of the major global health problems mainly in underdeveloped and developing world causing massive economic damage as well as distressing human life. Deficiency of useful micronutrients like vitamins and minerals including low level of availability of better quality protein causes hidden hunger which can be alleviate with the help of genetic bio-fortification of crops. Besides all the challenges, biofortified maize crops like quality protein maize along with the provitamin A and Zn hold a great future to address the malnutrition challenge combating the deficiency of malnutrtients. This is the most sustainable, cost-effective and potentially wide-reaching approach which can bridge the gap between agriculture and nutrition. Biofortification can be achieved both by agronomic and genetic approaches. The Importance, genetics and potential of bio-fotification is thoroughly reviewed to provide useful findings for new readers and researchers

Stem Cell Therapy for Post-Traumatic Stress Disorder: A Novel Therapeutic Approach

Stem cell therapy is a rapidly evolving field of regenerative medicine being employed for the management of various central nervous system disorders. The ability to self-renew, differentiate into specialized cells, and integrate into neuronal networks has positioned stem cells as an ideal mechanism for the treatment of epilepsy. Epilepsy is characterized by repetitive seizures caused by imbalance in the GABA and glutamate neurotransmission following neuronal damage. Stem cells provide benefit by reducing the glutamate excitotoxicity and strengthening the GABAergic inter-neuron connections. Similar to the abnormal neuroanatomic location in epilepsy, post-traumatic stress disorder (PTSD) is caused by hyperarousal in the amygdala and decreased activity of the hippocampus and medial prefrontal cortex. Thus, stem cells could be used to modulate neuronal interconnectivity. In this review, we provide a rationale for the use of stem cell therapy in the treatment of PTSD

Stem Cell Therapy for Post-Traumatic Stress Disorder: A Novel Therapeutic Approach

Stem cell therapy is a rapidly evolving field of regenerative medicine being employed for the management of various central nervous system disorders. The ability to self-renew, differentiate into specialized cells, and integrate into neuronal networks has positioned stem cells as an ideal mechanism for the treatment of epilepsy. Epilepsy is characterized by repetitive seizures caused by imbalance in the GABA and glutamate neurotransmission following neuronal damage. Stem cells provide benefit by reducing the glutamate excitotoxicity and strengthening the GABAergic inter-neuron connections. Similar to the abnormal neuroanatomic location in epilepsy, post-traumatic stress disorder (PTSD) is caused by hyperarousal in the amygdala and decreased activity of the hippocampus and medial prefrontal cortex. Thus, stem cells could be used to modulate neuronal interconnectivity. In this review, we provide a rationale for the use of stem cell therapy in the treatment of PTSD