68 research outputs found
Transplantacija autolognih matiÄnih stanica u lijeÄenju ne-Hodgkinovog limfoma
Substantial number of patients who present with non-Hodgkinās lymphoma cannot be cured of their disease by conventional dose therapy. New data on treatment results in the past decade elucidate the role of high-dose therapy (HDT) and autologous bone marrow or peripheral blood stem cells in the treatment of malignant lymphomas. There is evidence from randomized studies that high-dose therapy followed by autografting for relapsed chemosensitive patients is superior to conventional chemotherapy in terms of disease-free and overall survival. For this group of patients autografting became a standard approach to therapy. Also the increasing evidences indicate that high-dose therapy and autotransplantation in first remission improves survival in high-risk patients. The toxicity of the procedure is substantially reduced in recent years. Several new methods are under investigation, like various forms of immunotherapy and radioimmunotherapy, with the aim to reduce the incidence of relapse following transplantation.Brojne bolesnike s ne-Hodgkinovim limfomom nije moguÄe izlijeÄiti terapijom konvencionalnih doza. Novi podaci o rezultatima lijeÄenja u posljednjem desetljeÄu rasvjetljavaju ulogu terapije velikim dozama i matiÄnih stanica autlogne koÅ”tane srži ili periferne krvi u lijeÄenju zloÄudnih limfoma. U randomiziranim je ispitivanjima dokazano da je terapija velikim dozama nakon koje slijedi autotransplantacija primijenjena u kemosenzitivnih bolesnika s relapsom uspjeÅ”nija od konvencionalne kemoterapije s obzirom na sveukupno preživljenje bez znakova bolesti. Za tu je skuppinu bolesnika autotransplantacija postala standardna terapijska metoda. Sve je viÅ”e dokaza koji pokazuju da se terapijom velikim dozama i autotransplantacijom kod prve remisije bolesti postiže bolje preživljenje u bolesnika izloženim velikom riziku. ToksiÄnost postupka posljednjih je godina znatno smanjena. Ispituje se nekoliko novih metoda, poput raznih oblika imunoterapije i radioimunoterapije, s ciljem da se pojavnost relapsa nakon transplantacije smanji
Transplantacija autolognih matiÄnih stanica u lijeÄenju ne-Hodgkinovog limfoma
Substantial number of patients who present with non-Hodgkinās lymphoma cannot be cured of their disease by conventional dose therapy. New data on treatment results in the past decade elucidate the role of high-dose therapy (HDT) and autologous bone marrow or peripheral blood stem cells in the treatment of malignant lymphomas. There is evidence from randomized studies that high-dose therapy followed by autografting for relapsed chemosensitive patients is superior to conventional chemotherapy in terms of disease-free and overall survival. For this group of patients autografting became a standard approach to therapy. Also the increasing evidences indicate that high-dose therapy and autotransplantation in first remission improves survival in high-risk patients. The toxicity of the procedure is substantially reduced in recent years. Several new methods are under investigation, like various forms of immunotherapy and radioimmunotherapy, with the aim to reduce the incidence of relapse following transplantation.Brojne bolesnike s ne-Hodgkinovim limfomom nije moguÄe izlijeÄiti terapijom konvencionalnih doza. Novi podaci o rezultatima lijeÄenja u posljednjem desetljeÄu rasvjetljavaju ulogu terapije velikim dozama i matiÄnih stanica autlogne koÅ”tane srži ili periferne krvi u lijeÄenju zloÄudnih limfoma. U randomiziranim je ispitivanjima dokazano da je terapija velikim dozama nakon koje slijedi autotransplantacija primijenjena u kemosenzitivnih bolesnika s relapsom uspjeÅ”nija od konvencionalne kemoterapije s obzirom na sveukupno preživljenje bez znakova bolesti. Za tu je skuppinu bolesnika autotransplantacija postala standardna terapijska metoda. Sve je viÅ”e dokaza koji pokazuju da se terapijom velikim dozama i autotransplantacijom kod prve remisije bolesti postiže bolje preživljenje u bolesnika izloženim velikom riziku. ToksiÄnost postupka posljednjih je godina znatno smanjena. Ispituje se nekoliko novih metoda, poput raznih oblika imunoterapije i radioimunoterapije, s ciljem da se pojavnost relapsa nakon transplantacije smanji
Anemia and Other Manifestations of Iron, Vitamin B12 and Folate Deficiencies
Deficit željeza, vitamina B12 i folata dominantni
je uzrok anemije i velik je zdravstveni problem u zemljama
u razvoju, ali i u razvijenim zemljama diljem svijeta. Ovaj
pregled posveÄen je prevalenciji, metabolizmu i posebno
uzrocima i dijagnostici deficita ovih triju nutritivnih Äinitelja.
Anemija je dobro poznata i Äesto opisivana posljedica njihova
deficita, stoga Äe znatan dio prostora biti posveÄen novim
spoznajama o ulozi folata i vitamina B12 u kardiovaskularnim,
neuroloÅ”kim, psihijatrijskim i malignim bolestima, kao i u poremeÄajima razvoja. Napredak u istraživanju ovih nutritivnih
Äinitelja otkrio je nove aspekte hematoloÅ”kih poremeÄaja, ali
i mnoge nehematoloÅ”ke manifestacije te posebno blaga, pretkliniÄka
stanja deficita. Niz osjetljivih testova omoguÄava
ranu dijagnozu deficita vitamina i željeza. Visoka prevalencija
deficita otvorila je pitanje profilaktiÄke terapije i obogaÄivanja
hrane ovim vitaminima i mineralima, Ŕto je od posebne
važnosti za stariju populaciju i populaciju s niskim socioekonomskim
statusom.Deficiences of iron, vitamin B12 and folate
are the dominant causes of anemia and represent the major
public health problem in developing but also in developed
countries worldwide. This review is restricted to prevalence,
metabolism, and especially to causes and diagnosis of deficiency
of these three nutritients. Anemia is very well known
and frequently described consequence of their deficiency, and
consequently, more attention is paid to recent understanding
of the role of folate and vitamin B12 in cardiovascular, neurologic,
psychiatric and malignant diseases as well as developmental
abnormalities. Advances in the study of these nutritients
have revealed new aspects of hematological disturbances,
but also of many nonhematologic manifestations of
deficiency and especially mild, preclinical deficiency states. A
battery of sensitive tests allows for early detection of vitamin
and iron deficiency. The high prevalence of deficiency opened
a question of prophylactic therapy, and of food fortification
with these vitamins and minerals, especially in the elderly and
in people with low socioeconomic status
Fizioterapija bolesnika lijeÄenih transplantacijom krvotvornih matiÄnih stanica
Fizioterapija ima svoje mjesto u gotovo svim granama medicine, pa i kao dio lijeÄenja bolesnika s malignim bolestima. U ovome se radu razmatra uloga fizioterapije u bolesnika s hematoloÅ”kim malignim bolestima lijeÄenih transplantacijom krvotvornih matiÄnih stanica. Prikazan je tijek lijeÄenja transplantacijom krvotvornih matiÄnih stanica od pripreme za transplantaciju do razdoblja nakon transplantacije te problemi i komplikacije koje se pritom javljaju. LijeÄenje transplantacijom praÄeno je nizom komplikacija kao Å”to su promjene na
muskoloskeletnom sustavu te na kardiovaskularnom i respiratornom sustavu. Posebno je izražen problem akutne i kroniÄne reakcije transplantata protiv primatelja (graft versus host disease, GvHD). NaÄin lijeÄenja kojem je bolesnik podvrgnut i sve komplikacije rezultiraju
raznolikim ozbiljnim tjelesnim promjenama koje utjeÄu na fiziÄko stanje i kvalitetu života bolesnika, pa i na krajnji ishod lijeÄenja. Fizioterapija tih bolesnika iznimno je važna u svakom trenutku lijeÄenja, a njezini uÄinci na kvalitetu života bolesnika, smanjenje komplikacija
i konaÄni ishod lijeÄenja relativno su slabo istraženi. Zbog specifiÄnoga stanja i komplikacija nakon transplantacije hematoloÅ”kim je bolesnicima potreban jedinstven i složen pristup u fizioterapijskim procedurama. MeÄutim, ne postoje jasna uputstva i protokoli koje bi fizioterapeuti nužno slijedili i provodili u bolesnika lijeÄenih transplantacijom krvotvornih matiÄnih stanica. Opisana su dosadaÅ”nja iskustva i navedeni moguÄi pristupi fizioterapiji tih bolesnika
Adsorption of Arsenic on MgAl Layered Double Hydroxide
Groundwater in the Eastern Croatia, as well in the South-eastern Hungary, contains relatively high concentrations of arsenic that can cause chronic toxicity to humans. Therefore, the aim to find an ef-fective composite adsorbent that can be applied for arsenic water remediation by introducing it in the groundwater treatment is very important. The presented results were obtained using layered double hyĀ¬droxide (LDH) as a sorbing system. MgAl LDH samples with a Mg:Al molar ratio of 2:1 were syntheĀ¬sized. Adsorption of arsenic anions from groundwater samples from Eastern Croatia, as well as adsorption of model aquatic arsenic sample solutions, on MgAl layered double hydroxide was investigated. InducĀ¬tively coupled plasma atomic emission spectrometry (ICP-AES) was used for determination of arsenic concentration after adsorption. It was shown that in both cases the adsorption process could be interpreted in terms of Kroeker adsorption isotherm regardless to the presence of other ions in the groundwater. AddiĀ¬tionally, the influence of phosphate concentration on adsorption of model arsenic samples was examined and it was shown that (at least in examined range of arsenic and phosphate concentration) there is no sigĀ¬nificant influence of phosphate on adsorption of arsenic. (doi: 10.5562/cca2283
Collection and composition of autologous peripheral blood stem cells graft in patients with acute myeloid leukemia: influence on hematopoietic recovery and outcome [Skupljanje i sastav transplantata autolognih krvotvornih matiÄnih stanica periferne krvi u bolesnika s akutnom mijeloiÄnom leukemijom: utjecaj na hematoloÅ”ki oporavak i ishod]
Hematopoietic stem cell (HSC) transplantation is a standard approach in the treatment of hematological malignant diseases. For the last 15 years the main source of cells for trasplantation have been peripheral blood stem cells (PBSC). With the availability of hematopoietic growth factors and understanding the advantages of treatment with PBSC, the application of bone marrow (BM) was supplanted. The aim of this survey was to explore the success of PBSC collection, the factors which influence the success of PBSC collection, the composition and the quality of graft and their infuence on hematopoietic recovery and outcome after transplantation in patients with acute myeloid leukemia (AML). PBSC were collected by the method of leukapheresis after applying a combination of chemotherapy and growth factors or only growth factors. The quality of graft was determined with the clonogenic progenitor cell assay and with the flow citometry analysis. Of the total 134 patients with AML, who were submitted to HSC mobilization, the collection was successful in 78 (58.2%) patients. The collection was more successful after the first than after the second attempt of HSC mobilization (49% vs. 11%). The criteria for effective mobilization were the number of leukocytes >3Ā“109/L and the concentration of CD34+ cells >20Ā“103/mL in the peripheral blood on the first day of leukapheresis. The number of CD34+ cells infused had the strongest impact on hematopoietic recovery. We noted significantly faster hematological recovery of neutrophils and platelets, fewer number of transfused units of red blood cells and platelets, shorter duration of the tranfusion support, shorter treatment with intravenous antibiotic therapy and shorter hospitalization after PBSC compared to BM transplantation. These advantages could provide their standard application in the treatment of patients with AML
Smjernice za dijagnostiku i lijeÄenje primarne imunosne trombocitopenije u odraslih [Guidelines for diagnostics and treatment of primary immune thrombocytopenia in adults]
Working Group for Diseases of Hemostasis and Thrombosis of the Croatian Cooperative Group for Hematologic Diseases (CROHEM), Referral Center for Hereditary and Acquired Disorders of Hemostasis of the Croatian Ministry of Health, and Croatian Hematology Society of the Croatian Medical Association developed the Croatian guidelines for the diagnosis and treatment of primary immune thrombocytopenia (ITP). Detailed clinical and laboratory examination should be performed to exclude common secondary causes of thrombocytopenia. An individualized therapeutic approach is Āproposed, and decision to start active treatment depends on the severity and risk of bleeding, platelet count, patientās life style, age and comorbidities. Glucocorticoids with or without intravenous immunoglobulines are recommended as the first line treatment. The second line treatments are splenectomy or thrombopoietin receptor agonists as suggested options. Thrombopoietin receptor agonists are recommended treatment for splenectomized patients with relapsed ITP. Alternatively rituximab or other immunosuppressive agents are suggested in second or later treatment lines
GUIDELINES FOR DIAGNOSTICS AND TREATMENT OF PRIMARY IMMUNE THROMBOCYTOPENIA IN ADULTS
Radna skupina za bolesti hemostaze i tromboze Hrvatske kooperativne grupe za hematoloÅ”ke bolesti (KROHEM), Referentni centar Ministarstva zdravlja Republike Hrvatske za nasljedne i steÄene bolesti hemostaze te Hrvatsko hematoloÅ”ko druÅ”tvo Hrvatskoga lijeÄniÄkog zbora izradili su Hrvatske smjernice za dijagnostiku i lijeÄenje odraslih bolesnika s primarnom imunosnom trombocitopenijom (ITP). Detaljnim kliniÄkim i laboratorijskim pregledom treba iskljuÄiti niz Äestih uzroka sekundarne trombocitopenije. Predlaže se individualizirani pristup lijeÄenju, gdje se odluka o poÄetku aktivnog lijeÄenja temelji na ozbiljnosti i riziku od krvarenja, broju trombocita, životnom stilu, dobi bolesnika i komorĀbiditetima. Prva linija terapije ITP-a jesu glukokortikoidi, s primjenom intravenskih imunoglobulina ili bez nje. U drugoj terapijskoj liniji predlaže se splenektomija ili lijeÄenje agonistima trombopoetinskih receptora. Kod splenektomiranih Ābolesnika s relapsom ITP-a preporuÄuje se lijeÄenje agonistima trombopoetinskih receptora. Alternativno se sugerira moguÄnost primjene rituksimaba i drugih imunosupresiva u drugoj ili kasnijoj terapijskoj liniji.Working Group for Diseases of Hemostasis and Thrombosis of the Croatian Cooperative Group for Hematologic Diseases (CROHEM), Referral Center for Hereditary and Acquired Disorders of Hemostasis of the Croatian Ministry of Health, and Croatian Hematology Society of the Croatian Medical Association developed the Croatian guidelines for the diagnosis and treatment of primary immune thrombocytopenia (ITP). Detailed clinical and laboratory examination should be performed to exclude common secondary causes of thrombocytopenia. An individualized therapeutic approach is Āproposed, and decision to start active treatment depends on the severity and risk of bleeding, platelet count, patientās life style, age and comorbidities. Glucocorticoids with or without intravenous immunoglobulines are recommended as the first line treatment. The second line treatments are splenectomy or thrombopoietin receptor agonists as suggested options. Thrombopoietin receptor agonists are recommended treatment for splenectomized patients with relapsed ITP. Alternatively rituximab or other immunosuppressive agents are suggested in second or later treatment lines
Glycoprotein YKL-40: a novel biomarker of chronic graftvs- host disease activity and severity?
Aim To investigate whether increased YKL-40 levels positively
correlate with graft-vs-host disease (cGVHD) activity
and severity and if YKL-40 could serve as a disease biomarker.
Methods This case-control study was conducted at the
University Hospital Centre Zagreb from July 2013 to October
2015. 56 patients treated with hematopoietic stem
cell transplantation (HSCT) were included: 35 patients with
cGVHD and 21 without cGVHD. There was no difference
between groups in age, sex, median time from transplant
to study enrollment, intensity of conditioning, type of donor,
or source of stem cells. Blood samples were collected
at study enrollment and YKL-40 levels were measured with
ELISA. Disease activity was estimated using Clinicianās Impression
of Activity and Intensity of Immunosuppression
scales and disease severity using Global National Institutes
of Health (NIH) score.
Results YKL-40 levels were significantly higher in cGVHD
patients than in controls (P = 0.003). The difference remained
significant when patients with myelofibrosis were
excluded from the analysis (P = 0.017). YKL-40 level significantly
positively correlated with disease severity (P < 0.001;
correlation coefficient 0.455), and activity estimated using
Clinicianās Impression of Activity (P = 0.016; correlation coefficient
0.412) but not using Intensity of Immunosuppression
(P = 0.085; correlation coefficient 0.296).
Conclusion YKL-40 could be considered a biomarker of
cGVHD severity and activity. However, validation in a larger
group of patients is warranted, as well as longitudinal
testing of YKL-40 levels in patients at risk of developing
cGVHD
Which questionnaires should we use to evaluate quality of life in patients with chronic graft-vs-host disease?
Aim To investigate the ability of two standard quality of life
(QOL) questionnaires ā The Short Form (36-item) Health
Survey (SF-36) and The European Organisation for Research
and Treatment of Cancer Quality of Life Questionnaire-
Core 30 (EORTC QLQ C30) to evaluate QOL in patients with
chronic graft-vs-host disease (cGVHD) graded according to
National Institutes of Health (NIH) consensus criteria.
Methods In this cross-sectional study, QOL was assessed
in patients who underwent allogeneic stem cell transplantation
(allo-SCT) at the University Hospital Centre Zagreb
and were alive and in complete remission for more than
one year after allo-SCT.
Results The study included 58 patients, 38 patients with
cGVHD and 20 controls, patients without cGVHD. Patients
with cGVHD scored according to the NIH criteria had significantly
lower scores of global health status and lower
QOL on all SF-36 subscales and most of QLQ C30 functional
subscales (P < 0.050 for all comparisons). Furthermore,
patients with active cGVHD had significantly lower QOL
scores than patients with inactive cGVHD, and this difference
was most evident in physical functioning subscale of
SF-36 (P = 0.0007) and social functioning subscale of QLQ
C30 (P = 0.009).
Conclusion cGVHD scored according to the NIH criteria
is correlated with patient-reported QOL, particularly in the
physical domains as detected by SF-36. QLQ C30 questionnaire
adds more information on social functioning and
should be used as a valuable tool in the evaluation of social
domains in cGVHD patients
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