The match-play activity cycles in elite U17, U21 and senior hurling competitive games

Purpose The current study aimed to investigate the ball-in-play (BIP) and ball-out-of-play (BOP) differences between U17, U21 and senior hurling matches. Methods Video recordings of matches (n = 36) were coded and analysed for BIP and BOP. Time when the ball was continuously in-play was considered BIP, whereas any stoppages were considered BOP. Results The total and mean BIP cycle duration showed no difference between levels. The number of BIP cycles were higher in senior matches compared to U17 (ES = 1.80: large) and U21 (ES = 1.27: large). U17 matches had a lower frequency of BIP cycles between 16 and 30 s (ES = − 1.75: large) compared to senior. Total BOP duration was longer in senior (45:30 ± 4:13 min) matches compared to U17 (36:31 ± 2:30 min, ES = 2.59: very large) and U21 (36:48 ± 2:53 min, ES = 2.40: very large). Senior matches had a longer BOP duration and greater number of BOP cycles than U17 (ES = 0.17: trivial, ES = 2.20: very large, respectively) and U21 (ES = 0.17: trivial, ES = 0.99: moderate, respectively). U17 matches had a lower frequency of BOP cycles \u3e 60 s (ES = − 1.33: large) compared to senior. Conclusion Although there was a difference in the total match duration, U17 and U21 matches have similar BIP time as seniors, suggesting that U17 and U21 players should be conditioned to withstand the elite senior BIP duration. In training practice, high-intensity short-duration games are suggested for repeating the duration demands of competition

Lidar Ratios for Dust Aerosols Derived From Retrievals of CALIPSO Visible Extinction Profiles Constrained by Optical Depths from MODIS-Aqua and CALIPSO/CloudSat Ocean Surface Reflectance Measurements

CALIPSO's (Cloud Aerosol Lidar Infrared Pathfinder Satellite Observations) analysis algorithms generally require the use of tabulated values of the lidar ratio in order to retrieve aerosol extinction and optical depth from measured profiles of attenuated backscatter. However, for any given time or location, the lidar ratio for a given aerosol type can differ from the tabulated value. To gain some insight as to the extent of the variability, we here calculate the lidar ratio for dust aerosols using aerosol optical depth constraints from two sources. Daytime measurements are constrained using Level 2, Collection 5, 550-nm aerosol optical depth measurements made over the ocean by the MODIS (Moderate Resolution Imaging Spectroradiometer) on board the Aqua satellite, which flies in formation with CALIPSO. We also retrieve lidar ratios from night-time profiles constrained by aerosol column optical depths obtained by analysis of CALIPSO and CloudSat backscatter signals from the ocean surface

Identification of maximal running intensities during elite hurling match-play

The current study aimed to describe the duration-specific running intensities of elite hurling players during competition with respect to position using a rolling average method. Global positioning systems (10-Hz Viper; STATSport, Viper, Newry, Northern Ireland) were used to collect data from 36 elite hurling players across 2 seasons. Players were categorized according to playing positions (full-backs, half-backs, midfielders, half-forwards, and full-forwards). A total of 230 full match samples were obtained from 22 competitive games for analysis. The velocity-time curve was analyzed using a rolling average method, in which the maximum relative total distance (TD; m·min), high-speed running distance (HSR; m·min), and sprint distance (SD; m·min) intensities were calculated across 10 different rolling time durations (1-10 minutes) within each game. There were large to very large (effect sizes [ES] = 0.66-4.33) differences between 1 minute rolling averages and all other durations for TD, HSR, and SD. However, pairwise comparisons between 6 and 10 minutes for TD, HSR, and SD were smaller and more variable (ES = 0.07, trivial to ES = 0.85, moderate). Half-backs, midfielders, and half-forwards achieved a higher maximal relative TD and HSR in all duration-specific fields when compared with full-backs and full-forwards. No positional difference was observed in 1- and 2-minute durations for SD. Because the rolling average duration increased the maximum TD, HSR and SD running intensities decreased across all positions. These data provide knowledge of the peak running intensities of elite hurling competition and can be used to design training activities to sufficiently prepare players for these worst-case scenarios

Impact of the clusterization on the solubility of oxygen and vacancy concentration in nickel: A multi-scale approach

Combining thermodynamic concepts with first-principles calculations, we study the solubility of oxygen atoms (O) in nickel. In our approach, we include the possible formation of oxygen clusters (On) and vacancies-oxygens clusters (VOn and V2On). We show that the vacancy-oxygens interactions are strong (approximately 1 eV) and would induce a large concentration of clusters in fcc-Ni. The use of a thermodynamic model, within a grand canonical approach, allows calculation of the vacancy concentration, including these VOn clusters, as a function of O concentration, for different temperatures. We find that at low temperatures (below 600 K), a small content of oxygen (in appm) strongly modifies the vacancy concentration, increasing the total vacancy concentration in the metal by many orders of magnitude more than the thermal vacancy concentration. The vacancy concentration is thus directly controlled by the oxygen content in the metal. At high temperatures, the effect is reduced, becoming negligible near the melting point. These results show the strong impact of interstitial atoms on the vacancy concentration. The influence of the vacancy formation energy is also discussed

Huntingtin-associated protein 1: Eutherian adaptation from a TRAK-like protein, conserved gene promoter elements, and localization in the human intestine

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Background: Huntingtin-associated Protein 1 (HAP1) is expressed in neurons and endocrine cells, and is critical for postnatal survival in mice. HAP1 shares a conserved “HAP1_N” domain with TRAfficking Kinesin proteins TRAK1 and TRAK2 (vertebrate), Milton (Drosophila) and T27A3.1 (C. elegans). HAP1, TRAK1 and TRAK2 have a degree of common function, particularly regarding intracellular receptor trafficking. However, TRAK1, TRAK2 and Milton (which have a “Milt/TRAK” domain that is absent in human and rodent HAP1) differ in function to HAP1 in that they are mitochondrial transport proteins, while HAP1 has emerging roles in starvation response. We have investigated HAP1 function by examining its evolution, and upstream gene promoter sequences. We performed phylogenetic analyses of the HAP1_N domain family of proteins, incorporating HAP1 orthologues (identified by genomic synteny) from 5 vertebrate classes, and also searched the Dictyostelium proteome for a common ancestor. Computational analyses of mammalian HAP1 gene promoters were performed to identify phylogenetically conserved regulatory motifs. Results: We found that as recently as marsupials, HAP1 contained a Milt/TRAK domain and was more similar to TRAK1 and TRAK2 than to eutherian HAP1. The Milt/TRAK domain likely arose post multicellularity, as it was absent in the Dictyostelium proteome. It was lost from HAP1 in the eutherian lineage, and also from T27A3.1 in C. elegans. The HAP1 promoter from human, mouse, rat, rabbit, horse, dog, Tasmanian devil and opossum contained common sites for transcription factors involved in cell cycle, growth, differentiation, and stress response. A conserved arrangement of regulatory elements was identified, including sites for caudal-related homeobox transcription factors (CDX1 and CDX2), and myc-associated factor X (MAX) in the region of the TATA box. CDX1 and CDX2 are intestine-enriched factors, prompting investigation of HAP1 protein expression in the human duodenum. HAP1 was localized to singly dispersed mucosal cells, including a subset of serotonin-positive enterochromaffin cells. Conclusion: We have identified eutherian HAP1 as an evolutionarily recent adaptation of a vertebrate TRAK protein-like ancestor, and found conserved CDX1/CDX2 and MAX transcription factor binding sites near the TATA box in mammalian HAP1 gene promoters. We also demonstrated that HAP1 is expressed in endocrine cells of the human gut

The Match-Play Sprint Performance of Elite Senior Hurlers during Competitive Games

The typical sprint profile in elite hurling has yet to be established. The purpose of this study was to investigate the sprinting demands of elite hurling competition and characterize the sprinting patterns of different playing positions. GPS (10-Hz, STATSports Viper) were used to collect data from 51 hurlers during 18 games. The total sprint (≥22 km·h-1) distance (TSD), the number of sprints (NOS) classified as length (\u3c20 m, ≥20 m) and relative speed thresholds (\u3c80%, 80–90%, \u3e90%), the between-sprint duration and the number of repeated-sprint bouts (≥2 sprints in ≤60 s) were analyzed. The NOS was 22.2 ± 6.8 accumulating 415 ± 140 m TSD. The NOS \u3c20 m, ≥20 m was 14.0 ± 4.7 and 8.1 ± 3.6 respectively. The NOS \u3c80%, 80–90% and \u3e90% was 10.6 ± 4.3, 8.2 ± 3.6, 3.4 ± 2.4 respectively. The between-sprint duration and the repeated-sprint bouts were 208 ± 86 s and 4.5 ± 2.6 respectively. TSD (ES = -0.20), NOS (ES = -0.34), NOS \u3c20 m (ES = -0.33), ≥20 m (ES = -0.24), 80–90% (ES = -0.35) \u3e90% (ES = -0.13) and repeated-sprint bouts (ES = -0.28) decreased between-halves. Full-backs performed a lower NOS \u3c80% than half-backs (ES = -0.66) and a shorter mean duration of sprints than half-backs (ES = -0.75), midfielders (ES = -1.00) and full-forwards (ES = -0.59). These findings provide a sprint profile of elite hurling match-play that coaches should consider to replicate the sprint demands of competition in training

Metabolic Power in Hurling with Respect to Position and Halves of Match-play

The current investigation compared the metabolic power and energetic characteristics in team sports with respect to positional lines and halves of match-play. Global positioning system (GPS) technology data were collected from 22 elite competitive hurling matches over a 3-season period. A total of 250 complete match-files were recorded with players split into positional groups of full-back; half-back; midfield; half-forward; full-forward. Raw GPS data were exported into a customized spreadsheet that provided estimations of metabolic power and speed variables across match-play events (average metabolic power [Pmet], high metabolic load distance [HMLD], total distance, relative distance, high-speed distance, maximal speed, accelerations, and deceleration). Pmet, HMLD, total, relative and high-speed distance were 8.9±1.6 W�kg-1, 1457±349 m, 7506±1364 m, 107±20 m�min-1 and 1169± 260 m respectively. Half-backs, midfielders and half-forwards outperformed full-backs (Effect Size [ES] = 1.03, 1.22 and 2.07 respectively), and full-forwards in Pmet (Effect Size [ES] = 1.70, 2.07 and 1.28 respectively), and HMLD (full-backs: ES = -1.23, -1.37 and -0.84 respectively, and full-forwards: ES = -1.77, -2.00 and -1.38 respectively). Half-backs (ES = -0.60), midfielders (ES = -0.81), and half-forwards (ES = -0.74) experienced a second-half temporal decrement in HMLD. The current investigation demonstrates that metabolic power may increase our understanding of the match-play demands placed on elite hurling players. Coaches may utilize these findings to construct training drills that replicate match-play demands

The Regulation of Peripheral Metabolism by Gut-Derived Hormones

Enteroendocrine cells lining the gut epithelium constitute the largest endocrine organ in the body and secrete over 20 different hormones in response to cues from ingested foods and changes in nutritional status. Not only do these hormones convey signals from the gut to the brain via the gut-brain axis, they also act directly on metabolically important peripheral targets in a highly concerted fashion to maintain energy balance and glucose homeostasis. Gut-derived hormones released during fasting tend to be orexigenic and have hyperglycaemic potential. Conversely, gut hormones secreted postprandially generally promote satiety and facilitate glucose clearance. Although some of the metabolic benefits conferred by bariatric surgeries have been ascribed to changes in the secretory profiles of various gut hormones, the therapeutic potential of the enteroendocrine system as a viable target against metabolic diseases remain largely underexploited, except for incretin-mimetics. This review provides a brief overview of the physiological importance and highlights the therapeutic potential of the following gut hormones: serotonin, glucose-dependent insulinotropic peptide, glucagon-like peptide 1, oxyntomodulin, peptide YY, insulin-like peptide 5, and ghrelin

Regional differences in nutrient-induced secretion of gut serotonin

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Enterochromaffin (EC) cells located in the gastrointestinal (GI) tract provide the vast majority of serotonin (5‐HT) in the body and constitute half of all enteroendocrine cells. EC cells respond to an array of stimuli, including various ingested nutrients. Ensuing 5‐HT release from these cells plays a diverse role in regulating gut motility as well as other important responses to nutrient ingestion such as glucose absorption and fluid balance. Recent data also highlight the role of peripheral 5‐HT in various pathways related to metabolic control. Details related to the manner by which EC cells respond to ingested nutrients are scarce and as that the nutrient environment changes along the length of the gut, it is unknown whether the response of EC cells to nutrients is dependent on their GI location. The aim of the present study was to identify whether regional differences in nutrient sensing capability exist in mouse EC cells. We isolated mouse EC cells from duodenum and colon to demonstrate differential responses to sugars depending on location. Measurements of intracellular calcium concentration and 5‐HT secretion demonstrated that colonic EC cells are more sensitive to glucose, while duodenal EC cells are more sensitive to fructose and sucrose. Short‐chain fatty acids (SCFAs), which are predominantly synthesized by intestinal bacteria, have been previously associated with an increase in circulating 5‐HT; however, we find that SCFAs do not acutely stimulate EC cell 5‐HT release. Thus, we highlight that EC cell physiology is dictated by regional location within the GI tract, and identify differences in the regional responsiveness of EC cells to dietary sugars

The Maximal Intensity Period: Rationalising its Use in Team Sports Practice

Quantifying the highest intensity of competition (the maximal intensity period [MIP]) for varying durations in team sports has been used to identify training targets to inform the preparation of players. However, its usefulness has recently been questioned since it may still underestimate the training intensity required to produce specific physiological adaptations. Within this conceptual review, we aimed to: (i) describe the methods used to determine the MIP" (ii) compare the data obtained using MIP or whole-match analysis, considering the influence of different contextual factors" (iii) rationalise the use of the MIP in team sports practice and (iv) provide limitations and future directions in the area. Different methods are used to determine the MIP, with MIP values far greater than those derived from averaging across the whole match, although they could be affected by contextual factors that should be considered in practice. Additionally, while the MIP might be utilised during sport-specific drills, it is inappropriate to inform the intensity of interval-based, repeated sprint and linear speed training modes. Lastly, MIP does not consider any variable of internal load, a major limitation when informing training practice. In conclusion, practitioners should be aware of the potential use or misuse of the MIP