SDS–PAGE (a) and native PAGE (b) of purified laccase from <i>M.verrucaria</i> NF-05.

<p>(a) Lane 1: denatured protein marker, Lane 2: purified laccase; (b) Lane 1: purified laccase with Coomassie Brilliant Blue R-250staining, Lane 2: purified laccase with ABTS staining.</p

Substrate specificity of the purified laccase.

<p>Substrate specificity of the purified laccase.</p

UV–visible absorption (a) and electron paramagnetic resonance spectra (b) of laccase from <i>M.verrucaria</i> NF-05 in 10 mM citric acid buffer (pH 4.0)

<p>UV–visible absorption (a) and electron paramagnetic resonance spectra (b) of laccase from <i>M.verrucaria</i> NF-05 in 10 mM citric acid buffer (pH 4.0)</p

Characteristics of dyes tested in this work.

<p>Characteristics of dyes tested in this work.</p

Production of extracellular laccase by <i>M.verrucaria</i> NF-05.

<p>Results represents means of three experiments, and error bars indicates ± standard error.</p

Optima and stability of (a) temperature and (b) pH value for purified laccase from <i>M.verrucaria</i> NF-05 reacting with ABTS.

<p>Optima curve (•); stability curve (○). The highest value of activity for each analytical curve was considered as 100%, and error bars shown are standard deviations of triplicate samples.</p

Effect of inhibitors on laccase activity.

<p>Effect of inhibitors on laccase activity.</p

Decolourisation of dyes by <i>M.verrucaria</i> NF-05 laccase.

<p>Decolourisation of dyes by <i>M.verrucaria</i> NF-05 laccase.</p

Effect of different organic solvents (a)and enhancement effects of metal ions (b) on the activity of purified laccase from <i>M.verrucaria</i> NF-05.

<p>All the experiments are performed with the same purified laccase, and activity without the addition of organic solvents was considered as 100%. Error bars shown are standard errors of triplicate samples.</p

Degradation of azo dyes, a: orange I, b: amaranth, c: sudan II, d: sudan III, e: eriochrome black T; anthraquinone dyes, f: alizarin red, g: alizarin; arylmethane dyes, h: malachite green, i: fuchsin basic, j: crystal violet; and other dyes, k: methylen blue, l: phenol red by purified laccase from <i>M.verrucaria</i> NF-05.

<p>Degradation of azo dyes, a: orange I, b: amaranth, c: sudan II, d: sudan III, e: eriochrome black T; anthraquinone dyes, f: alizarin red, g: alizarin; arylmethane dyes, h: malachite green, i: fuchsin basic, j: crystal violet; and other dyes, k: methylen blue, l: phenol red by purified laccase from <i>M.verrucaria</i> NF-05.</p