Effect of expansion coefficient difference between machine tool and workpiece to the thermal deformation induced by room temperature change

9th CIRP Conference on High Performance Cutting (HPC 2020)In the precision machining process, ambient temperature is maintained to 20 °C to minimize the thermal deformations. Much energy is consumed to maintain ambient temperature. The use of thermal compensation systems can minimize the energy consumption of room cooling systems. However, the influence of thermal deformation induced by room temperature upon workpieces is not clear. This paper investigates the effect of the linear expansion coefficient difference between a machine tool and workpieces to the thermal deformation induced by room temperature change. Machining experiments are conducted for steel and aluminum workpieces. The results agree with the calculation

カバリング工程におけるカバード糸の被覆状態と糸張力に関する研究

取得学位：博士(工学)，学位授与番号：博甲第645号，学位授与年月日：平成16年3月25日,学位授与年：200

Detection of Mumps virus of Genotype G in Bangladeshi children suffering from Encephalitis

Although mumps virus (MuVi) is an important agent of encephalitis, however, mumps vaccine has not yet been included in the national immunization programme of Bangladesh. Furthermore, the genotype distribution of this virus in Bangladesh is unknown. Cerebrospinal fluid samples collected from 97 children with encephalitis from April 2009 to March 2010 were subjected to polymerase chain reaction (PCR) test to determine the causative agents. MuVi was detected in two samples, these samples were further subjected to conventional PCR using specific primers, then amplicons were sequenced, and genotype was determined as genotype G. Phylogenetic analysis showed that these strains were clustered with strains from Nepal, India, the UK, Thailand, and the USA. By Bayesian inference, we also determined that the ancestor of Bangladeshi and Indian MuVi were same and segregated only about two decades back. These results will help future surveillance and the detection of invading MuVi strains from other countries

Chemical Visualization of an Attractant Peptide, LURE

The pollen tube attractant peptide LUREs of Torenia fournieri are diffusible peptides that attract pollen tubes in vitro. Here, we report a method enabling the direct visualization of a LURE peptide without inhibiting its attraction activity by conjugating it with the Alexa Fluor 488 fluorescent dye. After purifying and refolding the recombinant LURE2 with a polyhistidine tag, its amino groups were targeted for conjugation with the Alexa Fluor dye. Labeling of LURE2 was confirmed by its fluorescence and mass spectrometry. In our in vitro assay using gelatin beads, Alexa Fluor 488-labeled LURE2 appeared to have the same activity as unlabeled LURE2. Using the labeled LURE2, the relationship between the spatiotemporal change of distribution and activity of LURE2 was examined. LURE2 attracted pollen tubes when embedded in gelatin beads, but hardly at all when in agarose beads. Direct visualization suggested that the significant difference between these conditions was the retention of LURE2 in the gelatin bead, which might delay diffusion of LURE2 from the bead. Direct visualization of LURE peptide may open the way to studying the spatiotemporal dynamics of LURE in pollen tube attraction