410 research outputs found
N\'eel-Dimer Transition in Antiferromagnetic Heisenberg Model and Deconfinement of Spinons at the Critical Point
Quantum phase transition from the N\'eel to the dimer states in an
antiferromagnetic(AF) Heisenberg model on square lattice is studied. We
introduce a control parameter for the exchange coupling which connects
the N\'eel () and the dimer () states. We employ the
(the Schwinger boson) representation of the spin operator and
integrate out the half of the variables at odd sites and we obtain a
nonlinear model. The effective coupling constant is a function
of and at the model is in the ordered phase which
corresponds to the N\'eel state of the AF Heisenberg model. A phase transition
to the dimer state occurs at a certain critical value of as
increases. In the N\'eel state, the dynamical composite U(1) gauge field in the
model is in a Higgs phase and low-energy excitations are gapless spin
wave. In the dimer phase, a confinement phase of the gauge theory is realized
and low-energy excitations are magnons. For the critical point, we argue
that a deconfinement phase, which is similar to the Coulomb phase in 3 spatial
dimensions, is realized and spinons appear as low-energy
excitations
In silico methods in enzyme screening and gene expression
INTMSAlign is a software to assign consensus residues of target protein utilizing large amount of their family sequences. We generated three protein sequences with S-selective hydroxynitrile lyase (S-HNL) activity, which we call designed S-HNLs; these proteins folded as efficiently as the native S-HNL (1). a-Amino-e-caprolactam (ACL) racemase from Achromobacter obae has been shown to be an effective catalyst for the dynamic kinetic resolution of amino acid amide and a-aminonitriles to form chiral amino acids. We searched for ACL racemase in silico with INTMSAlign software. By fixing Lys 241 as one of the key residues, we discovered thirteen ACL racemase genes from 413 fold type-I PLP genes (2).
Insolubility of proteins expressed in Escherichia coli expression hinders the progress of both basic and applied research. Insoluble proteins contain residues that decrease their solubility (aggregation hotspots). We discovered a phenomenon of soluble expression of HNL from Manihot esculenta, in E. coli. By random mutagenesis, we found that a single point mutation H103L, and mutation with alterations at three positions (Lys-Pro mutations at positions 176, 199 and 224) cause total solubility in E. coli even when grown at 37°C (3). If a relationship between soluble expression and mutation points could be established, it will become very easy to generate a mutant for correctly folded expression in E. coli. Using a combination of approaches involving directed evolution and primary sequence analysis, we found two rules of thumb to help identify hotspots: one focuses on the hydrophobicity of amino acids in the a-helix structure, and another one focuses the difference in hydrophobicity relative to the corresponding amino acid in the consensus protein. Using these two relationships together, we succeeded in developing methods to improve the solubility of expressed proteins in E. coli (4).
References:
(1) S. Nakano and Y. Asano, Sci. Rep., 5, 8193 (2015).
(2) W. Payoungkiattikun, S. Okazaki, S. Nakano, A. Ina, A. H-Kittikun, and Y. Asano, Appl. Biochem. Biotechnol., 176 (5), 1303-1314 (2015).
(3) Y. Asano, M. Dadashipour, M. Yamazaki, N. Doi, and H. Komeda. Prot. Eng. Des. Sel., 24 (8), 607-616 (2011).
(4) D. Matsui, S. Nakano, M. Dadashipour, and Y. Asano, submitted
Analysis of COVID-19 evolution based on testing closeness of sequential data
A practical algorithm has been developed for closeness analysis of sequential
data that combines closeness testing with algorithms based on the Markov chain
tester. It was applied to reported sequential data for COVID-19 to analyze the
evolution of COVID-19 during a certain time period (week, month, etc.)
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