1 research outputs found
Imaging Characterization of Cluster-Induced Morphological Changes of a Model Cell Membrane
Understanding
the activity of nanomaterials at the lipid bilayer
surface can provide key information for the feasible design of functional
bioactive agents. Herein, we used micro- and nanoscopic imaging techniques
to evaluate the activity of nanometer-sized inorganic clusters and
report that destruction of the lipid membrane is induced by a cluster-induced
morphological change on the membrane surface. As model experiments,
we used the Keggin-type polyoxometalate (POM) SiW<sub>12</sub>O<sub>40</sub><sup>4–</sup> for the inorganic cluster and a 1,2-dimyristoyl-<i>sn</i>-glycerol-3-phosphatidylcholine (DMPC) and egg phosphatidylcholine
(EPC) bilayer for the cell membrane. Imaging experiments revealed
vigorous desorption of the lipid bilayer from solid substrate by the
formation of POM–lipid assembly through a supramolecular-type
assembly process in which electrostatic and hydrophobic interactions
between the POM and lipid determine the efficiency and dynamics of
assembly formation and thereby determine lipid desorption. Furthermore,
maximum efficiency of lipid desorption was found at the phase-transition
temperature. This phase dependency was explained by the formation
of a “leaky interface” between the gel and fluid domains,
in which freedom in the conformational change of lipids during the
formation of the POM–lipid assemblies becomes maximal