Anti-inflammatory effects, nuclear magnetic resonance identification, and high-performance liquid chromatography isolation of the total flavonoids from Artemisia frigida

AbstractThe aerial parts of Artemisia frigida Willd. are used to treat joint swelling, renal heat, abnormal menstruation, and sore carbuncle. The anti-inflammatory effects of A. frigida have been well-known in folk medicine, suggesting that components extracted from A. frigida could potentially treat inflammatory disease. With the aim of discovering bioactive compounds, in this study, we extracted total flavonoids from the aerial parts of A. frigida and investigated their anti-inflammatory effects against inflammation induced by carrageenan and egg albumin in rats. At the doses studied, total flavonoids (100 mg/kg, 200 mg/kg, and 400 mg/kg) and some isolated compounds (30 mg/kg) showed significant and dose-dependent anti-inflammatory effects. According to the high-performance liquid chromatography analysis of the total flavonoids from A. frigida, there are five major compounds, namely, 5-hydroxy-3′,4′-dimethoxy-7-O-β-d-glucuronide (F1), 5-hydroxy-3′,4′,5′-trimethoxy-7-O-β-d-glucuronide (F2), 5,7,3′-trihydroxy-6,4′-dimethoxyflavone (F3), 5,3′-dihydroxy-6,7,4′-trimethoxyflavone (F4), and 5,3′-dihydroxy-3,6,7,4′-tetramethoxyflavone (F5), which may explain the anti-inflammatory activity

A new sesquiterpene from <i>Ixeris chinensis</i>

<div><p>The petroleum ether (PE) extract from <i>Ixeris chinensis</i> was submitted to chromatographic separation. A new sesquiterpene, namely 14-noreudesma-3-hydroxy-3-en-2,9-dione, was isolated from the PE extract. The structures of the sesquiterpene was elucidated by using spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.</p></div

A biflavonoid glycoside from <i>Lomatogonium carinthiacum</i> (Wulf) Reichb

<div><p>A biflavonoid glycoside with a new carbon skeleton, named carinoside A, was isolated from the <i>n</i>-BuOH crude extract of the whole plant of <i>Lomatogonium carinthiacum</i> (Wulf) Reichb. The structure of the new compound was elucidated by using spectroscopic methods, including UV, IR, HR-ESI-MS and extensive 1D and 2D NMR techniques.</p></div