1N3R-tau induces S phase arrest in HEK293 cells.

<p><b>(A)</b> The distribution of HEK293 with six tau isoforms in sub G1 phase. <b>(B)</b> The distribution of HEK293 with six tau isoforms in G1 phase. *<i>P</i> < 0.05, compared with vector. <b>(C)</b> The distribution of HEK293 with six tau isoforms in S phase. **<i>P</i> < 0.01, compared with vector. <b>(D)</b> The distribution of HEK293 with six tau isoforms in G2/M phase. *<i>P</i> < 0.05, compared with vector.</p

1N3R-tau inhibits cell proliferation measured by BrdU incorporation.

<p>Fluorescent micrographs of eGFP and BrdU expression with six tau isoforms in N2a cells after 48 h transfection. GFP (Green), BrdU (Red). Quantification of BrdU-positive cells in eGFP expressing N2a cells with six tau isoforms after 48 h transfection. Scale bar = 50 μm. All values are standardized with vector. *<i>P</i> < 0.05, compared with vector.</p

1N3R tau induces translocation of cyclin E from nucleus to cytoplasm.

<p><b>(A)</b> N2a cells were transfected transiently with full length tau (2N4R), 1N3R tau and empty vector for 48 h, then cytoplasm and nuclear proteins were extracted and cyclin A, cyclin B1, cyclin D1, cyclin E, Cdk2, Cdk4 were detected by western blotting. DM1A and Lamin B1 represented loading and nuclear reference respectively. <b>(B)</b> Quantitative analysis of (A). All values are standardized with vector. *<i>P</i> < 0.05, compared with vector.</p