Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model

BACKGROUND: Ulcerative colitis is a chronic inflammatory disease and involves multiple etiological factors. Acetic acid (AA)-induced colitis is a reproducible and simple model, sharing many characteristics with human colitis. N-acetylcysteine (NAC) has been widely used as an antioxidant in vivo and in vitro. NAC can affect several signaling pathways involving in apoptosis, angiogenesis, cell growth and arrest, redox-regulated gene expression, and inflammatory response. Therefore, NAC may not only protect against the direct injurious effects of oxidants, but also beneficially alter inflammatory events in colitis. This study was conducted to investigate whether NAC could alleviate the AA-induced colitis in a porcine model. METHODS: Weaned piglets were used to investigate the effects of NAC on AA-induced colitis. Severity of colitis was evaluated by colon histomorphology measurements, histopathology scores, tissue myeloperoxidase activity, as well as concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon. The protective role of NAC was assessed by measurements of antioxidant status, growth modulator, cell apoptosis, and tight junction proteins. Abundances of caspase-3 and claudin-1 proteins in colonic mucosae were determined by the Western blot method. Epidermal growth factor receptor, amphiregulin, tumor necrosis factor-alpha (TNF-α), and toll-like receptor 4 (TLR4) mRNA levels in colonic mucosae were quantified using the real-time fluorescent quantitative PCR. RESULTS: Compared with the control group, AA treatment increased (P < 0.05) the histopathology scores, intraepithelial lymphocyte (IEL) numbers and density in the colon, myeloperoxidase activity, the concentrations of malondialdehyde and pro-inflammatory mediators in the plasma and colon, while reducing (P < 0.05) goblet cell numbers and the protein/DNA ratio in the colonic mucosa. These adverse effects of AA were partially ameliorated (P < 0.05) by dietary supplementation with NAC. In addition, NAC prevented the AA-induced increase in caspase-3 protein, while stimulating claudin-1 protein expression in the colonic mucosa. Moreover, NAC enhanced mRNA levels for epidermal growth factor and amphiregulin in the colonic mucosa. CONCLUSION: Dietary supplementation with NAC can alleviate AA-induced colitis in a porcine model through regulating anti-oxidative responses, cell apoptosis, and EGF gene expression

ICT Changes Everything! But Who Changes ICT?

Information and communication technology (ICT) has a changing power and digitalization is gradually changing society in all aspects of life. Across the western world, men are in majority in the ICT industry, thus, the computer programs that change “everything” are most often made by men. Unless questioned, this male dominance can be perceived as a “norm” and becomes invisible. Against this background, this paper will provide three examples of how a feminist gaze can contribute to raise important questions and produce an awareness of how exclusion mechanisms have produce a highly homosocial tendency in design of ICT systems in the western world. The three cases illustrate how a feminist gaze leading to feminist interventions can make a difference in various ways. The first author presents a case study of a pilot for involving programming in public education in secondary schools in Norway, where a complete lack of gender awareness makes this an offer for boys in most schools. Author two presents a case study comparing the situation in the IT business in the UK and India, finding challenges not only to the situation in the western world, but also to white western feminism. Author three discusses alternative ways of involving women in ICT work, through practices of feminist pedagogy, emphasizing hands-on work

Magnetic susceptibility and VLF-R investigations for determining geothermal blowout contaminated area: a case study from Alaşehir (Manisa/Turkey)

Western Turkey is one of the most spectacular regions of widespread, active continental extension in the world. The most prominent structures of this region are E-W trending Gediz and Buyuk Menderes Grabens. Geothermal activity around city of Manisa in Gediz Graben has been investigated by many researchers and many geothermal boreholes were drilled in order to produce electricity. In the middle of May 2012, a geothermal blowout occurred 150 m away from the deep drilling (depth 1,100 m), in Alkan village of AlaAYehir resort in city of Manisa. After that, four big blowouts took place at the same area and thermal water had been diverted to Alkan stream for almost 4 months. The boron (B) and Fe levels of thermal water are 87 and 11.2 ppm where the background B and Fe values of topsoil vary between 0.2-1.5 ppm and 3.08-5.63 ppm, respectively. The objective of this study was to utilize joint magnetic susceptibility and VLF-R investigations to determine possibly contaminated area with B, related to high Fe level on the topsoil and thermal water in the subterranean layers. It is observed that magnetic susceptibility values vary between 500 and 790 cgs (x10(-6)) in the vicinity of blowouts and 150-340 cgs (x10(-6)) at a distance of 50 m around, where the background average value is 20 cgs (x10(-6)), by using the data collected at 346 stations. Besides, the VLF-R studies were carried out along 18 profiles at three different frequencies. Laterally constrained two-layer inversion was applied to each station; in addition to the inversion of all profiles for each frequency, all lines were stacked, 2-D resistivity maps were obtained, and the contaminated area was determined approximately as 35,000 m(2). The results show not only satisfactory agreement between magnetic susceptibility and VLF-R data, but also provide fast and valued interpretation of geochemical and geophysical properties of the geothermal contaminated area