Avaliação de progênies derivadas de cruzamentos envolvendo o acesso com espiguetas agrupadas da Embrapa.

O presente trabalho teve como objetivo realizar a avaliação de progênies F3:5 provenientes de cruzamentos biparentais envolvendo cultivares/linhagens-elite e o acesso com espiguetas agrupadas da Embrapa, com base no caráter produção de grãos

Avaliação das diferentes práticas de manejo na agregação do solo sob cultivo orgânico de hortaliças em Seropédica (RJ).

Anais do XI Congresso Brasileiro de Agroecologia, São Cristóvão, Sergipe

Utilização integrada do radar de penetração do solo (GPR) e gamaespectrômetro como subsídio na identificação da transição entre um Planossolo Háplico e um Argissolo Vermelho.

O objetivo deste trabalho foi integrar dois métodos geofísicos distintos (GPR e gamaespectrômetro) na delimitação entre dois solos contrastantes. O estudo ocorreu em uma vertente na Fazendinha Agroecológica Km 47, em Seropédica-RJ. Assim, foram descritos dois perfis (Planossolo Háplico e Argissolo Vermelho) nas extremidades de um transecto de 70 metros. Em seguida, foram colocadas barras de ferro nas transições dos horizontes para demarcar suas profundidades no radargrama. Utilizou-se o GPR portando uma antena monoestática blindada de 450 MHz e gamaespectrômetro (leitura dos teores de tório equivalente) ao longo do transecto. Como resultado, o final do horizonte E do Planossolo no radargrama do transecto coincidiu com o aumento das radiações de tório equivalente na parte mais alta e argilosa do terreno (zona do Argissolo). Tradagens confirmaram a transição entre os solos indicada nas imagens. Assim, os métodos integrados apresentaram relevante aferição na transição do solo de textura arenosa para argilosa

LeishVet update and recommendations on feline leishmaniosis

Limited data is available on feline leishmaniosis (FeL) caused by Leishmania infantum worldwide. The LeishVet group presents in this report a review of the current knowledge on FeL, the epidemiological role of the cat in L. infantum infection, clinical manifestations, and recommendations on diagnosis, treatment and monitoring, prognosis and prevention of infection, in order to standardize the management of this disease in cats. The consensus of opinions and recommendations was formulated by combining a comprehensive review of evidence-based studies and case reports, clinical experience and critical consensus discussions. While subclinical feline infections are common in areas endemic for canine leishmaniosis, clinical illness due to L. infantum in cats is rare. The prevalence rates of feline infection with L. infantum in serological or molecular-based surveys range from 0 % to more than 60 %. Cats are able to infect sand flies and, therefore, they may act as a secondary reservoir, with dogs being the primary natural reservoir. The most common clinical signs and clinicopathological abnormalities compatible with FeL include lymph node enlargement and skin lesions such as ulcerative, exfoliative, crusting or nodular dermatitis (mainly on the head or distal limbs), ocular lesions (mainly uveitis), feline chronic gingivostomatitis syndrome, mucocutaneous ulcerative or nodular lesions, hypergammaglobulinaemia and mild normocytic normochromic anaemia. Clinical illness is frequently associated with impaired immunocompetence, as in case of retroviral coinfections or immunosuppressive therapy. Diagnosis is based on serology, polymerase chain reaction (PCR), cytology, histology, immunohistochemistry (IHC) or culture. If serological testing is negative or low positive in a cat with clinical signs compatible with FeL, the diagnosis of leishmaniosis should not be excluded and additional diagnostic methods (cytology, histology with IHC, PCR, culture) should be employed. The most common treatment used is allopurinol. Meglumine antimoniate has been administered in very few reported cases. Both drugs are administered alone and most cats recover clinically after therapy. Follow-up of treated cats with routine laboratory tests, serology and PCR is essential for prevention of clinical relapses. Specific preventative measures for this infection in cats are currently not available

Tyrosinase-catalyzed site-specific immobilization of engineered C-phycocyanin to surface

Enzymatic crosslinking of proteins is often limited by the steric availability of the target residues, as of tyrosyl side chains in the case of tyrosinase. Carrying an N-terminal peptide-tag containing two tyrosine residues, the fluorescent protein C-phycocyanin HisCPC from Synechocystis sp. PCC6803 was crosslinked to fluorescent high-molecular weight forms with tyrosinase. Crosslinking with tyrosinase in the presence of L-tyrosine produced non fluorescent high-molecular weight products. Incubated in the presence of tyrosinase, HisCPC could also be immobilized to amino-modified polystyrene beads thus conferring a blue fluorescence. Crosslinking and immobilization were site-specific as both processes required the presence of the N-terminal peptide in HisCPC