29 research outputs found
Propionato de cĂĄlcio no amaciamento do mĂșsculo Longissimus dorsi de bovinos de corte
Effect of energy concentration in the diets on sensorial and chemical parameters of Morada Nova, Santa Inez and Santa Inez x Dorper lamb meat
Meat quality of Santa InĂȘs sheep raised in confinement with diet containing cactus pear replacing corn
Fatty acid composition of the longissimus dorsi muscle in crossbred steers fed different sources of fatty acids
Lipid and selenium sources on fatty acid composition of intramuscular fat and muscle selenium concentration of Nellore steers
Desempenho e caracterĂsticas de carcaça de novilhos alimentados com dietas contendo diferentes fontes de lipĂdios
CaracterĂsticas de carcaça e qualidade da carne de novilhas alimentadas com silagem de capim-marandu
Perfil aromĂĄtico e qualidade quĂmica da carne de caprinos Saanen alimentados com diferentes nĂveis de concentrado
Impact of fetal exposure to mycotoxins on longissimus muscle fiber hypertrophy and miRNA profile
Abstract Background Longissimus muscle samples were collected from lambs exposed in utero to mycotoxins [E-, endophyte-free tall fescue seed without ergot alkaloids (negative control) or E + , endophyte-infected tall fescue seed containing ergot alkaloids] during mid-gestation (MID; E + /E-) or late-gestation (LATE; E-/E +) harvested at two developmental stages (FETAL, gestational d133) or (MAT, near maturity, 250 d of age; n = 3/treatment/developmental stage). Muscle samples were examined to determine the impact of in utero mycotoxin exposure on skeletal muscle fiber hypertrophy and the miRNA profile at FETAL and MAT. Results Longissimus weight was greater (P \u3c 0.05) in E + /E- lambs compared to E-/E + lambs at MAT; however, FETAL longissimus weight did not differ (P \u3e 0.10) between fescue treatments. Type I fiber cross sectional area was larger (P \u3c 0.10) for E + /E- than E-/E + at MAT but did not differ (P \u3e 0.10) between fescue treatments at FETAL. Type II fiber area was larger (P \u3c 0.05) at MAT in E + /E- compared to E-/E + but did not differ (P \u3c 0.05) between fescue treatments at FETAL. Cross-sectional Type I and Type II longissimus muscle fiber area increased (P \u3c 0.05) from FETAL to MAT by 6.86-fold and 10.83-fold, respectively. The ratio of Type II:Type I muscle fibers was lower (P = 0.04) at MAT compared to FETAL. There were 120 miRNA differentially expressed (q \u3c 0.05) between FETAL and MAT. Maternal fescue treatment did not alter (q \u3e 0.05) expression of miRNAs in the longissimus muscle. miR-133, -29a, -22-3p, and -410-3p were identified as highly significant with a log2 fold change \u3e 4. In vitro satellite cell cultures showed that selected miRNAs (miR-22-3p, 29a, 27a, and 133a) are differentially regulated during proliferation and differentiation indicating a role of miRNA in muscle hypertrophy. Conclusions Exposure to mycotoxins did not alter fiber type but had long-term impacts on postnatal muscle hypertrophy and cross-sectional area. The miRNA profile of the longissimus was not altered by Maternal mycotoxin exposure at FETAL or MAT. Developmental age altered the miRNA transcriptome and mRNA expression of known genes related to muscle growth. These results indicate that Maternal exposure to E + fescue seed during LATE gestation can alter postnatal muscle hypertrophy in sheep; however, these changes are not regulated by the miRNA transcriptome of the longissimus muscle