1,341 research outputs found
Folate catabolites in spot urine as non-invasive biomarkers of folate status during habitual intake and folic acid supplementation.
Folate status, as reflected by red blood cell (RCF) and plasma folates (PF), is related to health and disease risk. Folate degradation products para-aminobenzoylglutamate (pABG) and para-acetamidobenzoylglutamate (apABG) in 24 hour urine have recently been shown to correlate with blood folate.
Since blood sampling and collection of 24 hour urine are cumbersome, we investigated whether the determination of urinary folate catabolites in fasted spot urine is a suitable non-invasive biomarker for folate status in subjects before and during folic acid supplementation.
Immediate effects of oral folic acid bolus intake on urinary folate catabolites were assessed in a short-term pre-study. In the main study we included 53 healthy men. Of these, 29 were selected for a 12 week folic acid supplementation (400 µg). Blood, 24 hour and spot urine were collected at baseline and after 6 and 12 weeks and PF, RCF, urinary apABG and pABG were determined.
Intake of a 400 µg folic acid bolus resulted in immediate increase of urinary catabolites. In the main study pABG and apABG concentrations in spot urine correlated well with their excretion in 24 hour urine. In healthy men consuming habitual diet, pABG showed closer correlation with PF (rs = 0.676) and RCF (rs = 0.649) than apABG (rs = 0.264, ns and 0.543). Supplementation led to significantly increased folate in plasma and red cells as well as elevated urinary folate catabolites, while only pABG correlated significantly with PF (rs = 0.574) after 12 weeks.
Quantification of folate catabolites in fasted spot urine seems suitable as a non-invasive alternative to blood or 24 hour urine analysis for evaluation of folate status in populations consuming habitual diet. In non-steady-state conditions (folic acid supplementation) correlations between folate marker (RCF, PF, urinary catabolites) decrease due to differing kinetics
Pheno4J: a gene to phenotype graph database
Efficient storage and querying of large amounts of genetic and phenotypic data is crucial to contemporary clinical genetic research. This introduces computational challenges for classical relational databases, due to the sparsity and sheer volume of the data. Our Java based solution loads annotated genetic variants and well phenotyped patients into a graph database to allow fast efficient storage and querying of large volumes of structured genetic and phenotypic data. This abstracts technical problems away and lets researchers focus on the science rather than the implementation. We have also developed an accompanying webserver with end-points to facilitate querying of the database
Gauss-Bonnet Black Holes and Heavy Fermion Metals
We consider charged black holes in Einstein-Gauss-Bonnet Gravity with
Lifshitz boundary conditions. We find that this class of models can reproduce
the anomalous specific heat of condensed matter systems exhibiting
non-Fermi-liquid behaviour at low temperatures. We find that the temperature
dependence of the Sommerfeld ratio is sensitive to the choice of Gauss-Bonnet
coupling parameter for a given value of the Lifshitz scaling parameter. We
propose that this class of models is dual to a class of models of
non-Fermi-liquid systems proposed by Castro-Neto et.al.Comment: 17 pages, 6 figures, pdfLatex; small corrections to figure 10 in this
versio
Pituitary apoplexy can mimic acute meningoencephalitis or subarachnoid haemorrhage
Pituitary apoplexy is an uncommon but life-threatening condition that is often overlooked and underdiagnosed. We report a 45-year-old man who presented to our emergency department with a sudden onset headache, acute confusion, signs of meningeal irritation and ophthalmoplegia. An initial diagnosis of acute meningoencephalitis was made, which was amended to pituitary apoplexy following thorough investigation within the emergency department
Lifshitz-like space-time from intersecting branes in string/M theory
We construct 1/4 BPS, threshold F-D bound states (with )
of type II string theories by applying S- and T-dualities to the D1-D5 system
of type IIB string theory. These are different from the known 1/2 BPS,
non-threshold F-D bound states. The near horizon limits of these solutions
yield Lifshitz-like space-times with varying dynamical critical exponent
, for , along with the hyperscaling violation exponent
, showing how Lifshitz-like space-time can be
obtained from string theory. The dilatons are in general non-constant (except
for ). We discuss the holographic RG flows and the phase structures of
these solutions. For , we do not get a Lifshitz-like space-time, but the
near horizon limit in this case leads to an AdS space.Comment: 20 pages, no figure, v2: proper identification of hyperscaling
violation exponent has been made, abstract and the text has been changed
accordingly, note added, v3: minor changes, refs added, version to appear in
JHE
Aspects of holography for theories with hyperscaling violation
We analyze various aspects of the recently proposed holographic theories with
general dynamical critical exponent z and hyperscaling violation exponent
. We first find the basic constraints on from the gravity
side, and compute the stress-energy tensor expectation values and scalar
two-point functions. Massive correlators exhibit a nontrivial exponential
behavior at long distances, controlled by . At short distance, the
two-point functions become power-law, with a universal form for .
Next, the calculation of the holographic entanglement entropy reveals the
existence of novel phases which violate the area law. The entropy in these
phases has a behavior that interpolates between that of a Fermi surface and
that exhibited by systems with extensive entanglement entropy. Finally, we
describe microscopic embeddings of some metrics into full
string theory models -- these metrics characterize large regions of the
parameter space of Dp-brane metrics for . For instance, the theory of
N D2-branes in IIA supergravity has z=1 and over a wide range
of scales, at large .Comment: 35 pages; v2: new references added; v3: proper reference [14] added;
v4: minor clarification
Global biodiversity monitoring: From data sources to Essential Biodiversity Variables
Essential Biodiversity Variables (EBVs) consolidate information from varied biodiversity observation sources. Here we demonstrate the links between data sources, EBVs and indicators and discuss how different sources of biodiversity observations can be harnessed to inform EBVs. We classify sources of primary observations into four types: extensive and intensive monitoring schemes, ecological field studies and satellite remote sensing. We characterize their geographic, taxonomic and temporal coverage. Ecological field studies and intensive monitoring schemes inform a wide range of EBVs, but the former tend to deliver short-term data, while the geographic coverage of the latter is limited. In contrast, extensive monitoring schemes mostly inform the population abundance EBV, but deliver long-term data across an extensive network of sites. Satellite remote sensing is particularly suited to providing information on ecosystem function and structure EBVs. Biases behind data sources may affect the representativeness of global biodiversity datasets. To improve them, researchers must assess data sources and then develop strategies to compensate for identified gaps. We draw on the population abundance dataset informing the Living Planet Index (LPI) to illustrate the effects of data sources on EBV representativeness. We find that long-term monitoring schemes informing the LPI are still scarce outside of Europe and North America and that ecological field studies play a key role in covering that gap. Achieving representative EBV datasets will depend both on the ability to integrate available data, through data harmonization and modeling efforts, and on the establishment of new monitoring programs to address critical data gaps
[89Zr]Oxinate4 for long-term in vivo cell tracking by positron emission tomography
Purpose 111In (typically as [111In]oxinate3) is a gold standard
radiolabel for cell tracking in humans by scintigraphy. A long
half-life positron-emitting radiolabel to serve the same purpose
using positron emission tomography (PET) has long
been sought. We aimed to develop an 89Zr PET tracer for cell
labelling and compare it with [111In]oxinate3 single photon
emission computed tomography (SPECT).
Methods [89Zr]Oxinate4 was synthesised and its uptake and
efflux were measured in vitro in three cell lines and in human
leukocytes. The in vivo biodistribution of eGFP-5T33 murine
myeloma cells labelled using [89Zr]oxinate4 or [111In]oxinate3
was monitored for up to 14 days. 89Zr retention by living
radiolabelled eGFP-positive cells in vivo was monitored by
FACS sorting of liver, spleen and bone marrow cells followed
by gamma counting.
Results Zr labelling was effective in all cell types with yields
comparable with 111In labelling. Retention of 89Zr in cells
in vitro after 24 h was significantly better (range 71 to
>90 %) than 111In (43–52 %). eGFP-5T33 cells in vivo
showed the same early biodistribution whether labelled with
111In or 89Zr (initial pulmonary accumulation followed by
migration to liver, spleen and bone marrow), but later translocation
of radioactivity to kidneys was much greater for 111In.
In liver, spleen and bone marrow at least 92 % of 89Zr
remained associated with eGFP-positive cells after 7 days
in vivo.
Conclusion [89Zr]Oxinate4 offers a potential solution to the
emerging need for a long half-life PET tracer for cell tracking
in vivo and deserves further evaluation of its effects on survival
and behaviour of different cell types
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