19 research outputs found
Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal
Abstract: In this study, we report on the bacterial community associated with the pinewood nematode Bursaphelenchus xylophilus from symptomatic pine wilted trees, as well as from long-term preserved B. xylophilus laboratory collection specimens, emphasizing the close bacteria-nematode associations that may contribute to pine wilt disease development
Bacteria associated with the pinewood nematode Bursaphelenchus xylophilus collected in Portugal
Nematicidal Bacteria Associated to Pinewood Nematode Produce Extracellular Proteases
Bacteria associated with the nematode Bursaphelenchus xylophilus, a pathogen of trees and the causal agent of
pine wilt disease (PWD) may play a role in the disease. In order to evaluate their role (positive or negative to the
tree), strains isolated from the track of nematodes from infected Pinus pinaster trees were screened, in vitro, for their
nematicidal potential. The bacterial products, from strains more active in killing nematodes, were screened in order to
identify and characterize the nematicidal agent. Forty-seven strains were tested and, of these, 21 strains showed
capacity to produce extracellular products with nematicidal activity. All Burkholderia strains were non-toxic. In
contrast, all Serratia strains except one exhibited high toxicity. Nematodes incubated with Serratia strains showed, by
SEM observation, deposits of bacteria on the nematode cuticle. The most nematicidal strain, Serratia sp. A88copa13,
produced proteases in the supernatant. The use of selective inhibitors revealed that a serine protease with 70 kDa
was majorly responsible for the toxicity of the supernatant. This extracellular serine protease is different
phylogenetically, in size and biochemically from previously described proteases. Nematicidal assays revealed
differences in nematicidal activity of the proteases to different species of Bursaphelenchus, suggesting its usefulness
in a primary screen of the nematodes. This study offers the basis for further investigation of PWD and brings new
insights on the role bacteria play in the defense of pine trees against B. xylophilus. Understanding all the factors
involved is important in order to develop strategies to control B. xylophilus dispersion.This research was partially supported by Direcção Regional de Florestas, Fundo Florestal Permanente and Autoridade Florestal Nacional,
through a national project ‘O nemátode-da-madeira-do-pinheiro (NMP), Bursaphelenchus xylophilus’ and by FEDER funds through the Programa
Operacional Factores de Competitividade – COMPETE and by national funds through the Fundação para a Ciência e Tecnologia (FCT), Portugal, under
the project PTDC/AGR-CFL/115373/2009. D.N.P. was supported by FCT, Portugal, graduate fellowship SFRH/BD/61311/2009. G.P. was supported by
FCT, Portugal, fellowship PTDC/AGR-CFL/115373/2009
Diversity of Bacteria Carried by Pinewood Nematode in USA and Phylogenetic Comparison with Isolates from Other Countries
Bacterial Diversity and Community Structure in the Pine Wood Nematode Bursaphelenchus xylophilus and B. mucronatus with Different Virulence by High-Throughput Sequencing of the 16S rDNA
The Microbiome of Endophytic, Wood Colonizing Bacteria from Pine Trees as Affected by Pine Wilt Disease
Metagenomic analysis of the pinewood nematode microbiome reveals a symbiotic relationship critical for xenobiotics degradation
Combining microfluidics and RNA-sequencing to assess the inducible defensome of a mushroom against nematodes
Exploring the consumption of charity-linked products
Cause-related marketing, Charity-linked products, Social marketing, Nonprofit organizations, Consumer behavior,