Adapting feedback types according to students’ affective states

Affective states play a significant role in students’ learning behaviour. Positive affective states can enhance learning, while negative ones can inhibit it. This paper describes the development of an affective state reasoner that is able to adapt the feedback type according to students’ affective states in order to evoke positive affective states and as such improve their learning experience. The reasoner relies on a dynamic Bayesian network trained with data gathered in a series of ecologically valid Wizard-of-Oz studies, where the effect of feedback on students’ affective states was investigated

A simple dummy liver assist device prolongs anhepatic survival in a porcine model of total hepatectomy by slight hypothermia

<p>Abstract</p> <p>Background</p> <p>Advances in intensive care support such as therapeutic hypothermia or new liver assist devices have been the mainstay of treatment attempting to bridge the gap from acute liver failure to liver transplantation, but the efficacy of the available devices in reducing mortality has been questioned. To address this issue, the present animal study was aimed to analyze the pure clinical effects of a simple extracorporeal dummy device in an anhepatic porcine model of acute liver failure.</p> <p>Methods</p> <p>Total hepatectomy was performed in ten female pigs followed by standardized intensive care support until death. Five animals (dummy group, n = 5) underwent additional cyclic connection to an extracorporeal dummy device which consisted of a plasma separation unit. The separated undetoxified plasma was completely returned to the pigs circulation without any plasma substitution or exchange in contrast to animals receiving intensive care support alone (control group, n = 5). All physiological parameters such as vital and ventilation parameters were monitored electronically; laboratory values and endotoxin levels were measured every 8 hours.</p> <p>Results</p> <p>Survival of the dummy device group was 74 ± 6 hours in contrast to 53 ± 5 hours of the control group which was statistically significant (p < 0.05). Body temperature 24 hours after hepatectomy was significantly lower (36.5 ± 0.5°C vs. 38.2 ± 0.7°C) in the dummy device group. Significant lower values were measured for blood lactate (1.9 ± 0.2 vs. 2.5 ± 0.5 mM/L) from 16 hours, creatinine (1.5 ± 0.2 vs. 2.0 ± 0.3 mg/dL) from 40 hours and ammonia (273 ± 122 vs. 1345 ± 700 μg/dL) from 48 hours after hepatectomy until death. A significant rise of endotoxin levels indicated the onset of sepsis at time of death in 60% (3/5) of the dummy device group animals surviving beyond 60 hours from hepatectomy.</p> <p>Conclusions</p> <p>Episodes of slight hypothermia induced by cyclic connection to the extracorporeal dummy device produced a significant survival benefit of more than 20 hours through organ protection and hemodynamic stabilisation. Animal studies which focus on a survival benefit generated by liver assist devices should especially address the aspect of slight transient hypothermia by extracorporeal cooling.</p

STING Agonist 8803 Reprograms the Immune Microenvironment and Increases Survival in Preclinical Models of Glioblastoma

STING agonists can reprogram the tumor microenvironment to induce immunological clearance within the central nervous system. Using multiplexed sequential immunofluorescence (SeqIF) and the Ivy Glioblastoma Atlas, STING expression was found in myeloid populations and in the perivascular space. The STING agonist 8803 increased median survival in multiple preclinical models of glioblastoma, including QPP8, an immune checkpoint blockade-resistant model, where 100% of mice were cured. Ex vivo flow cytometry profiling during the therapeutic window demonstrated increases in myeloid tumor trafficking and activation, alongside enhancement of CD8+ T cell and NK effector responses. Treatment with 8803 reprogrammed microglia to express costimulatory CD80/CD86 and iNOS, while decreasing immunosuppressive CD206 and arginase. In humanized mice, where tumor cell STING is epigenetically silenced, 8803 therapeutic activity was maintained, further attesting to myeloid dependency and reprogramming. Although the combination with a STAT3 inhibitor did not further enhance STING agonist activity, the addition of anti-PD-1 antibodies to 8803 treatment enhanced survival in an immune checkpoint blockade-responsive glioma model. In summary, 8803 as a monotherapy demonstrates marked in vivo therapeutic activity, meriting consideration for clinical translation

JavaScript and JSON essentials: build light weight, scalable, and faster web applications with the power of JSON

JSON is an established and standard format used to exchange data. This book shows how JSON plays different roles in full web development through examples. By the end of this book, you'll have a new perspective on providing solutions for your applications and handling their complexities

Expression and content of terminal oxidases in Azotobacter vinelandii grown with excess NH4+ are modulated by O-2 supply

The influence of the rate of O2 supply to batch cultures on the contents of cytochromes bd and 'o' in NH4 +-grown Azotobacter vinelandii has been investigated. Difference spectra at room temperature (reduced + CO minus reduced) were recorded for whole cells of a wild-type strain and mutants which either lacked or over-produced the cytochrome bd-type terminal oxidase encoded by cydAB. A Tn5-B20 insertion in cydB in the former mutant also provided a means of monitoring cydAB gene expression from measurements of β-galactosidase activity. The content of cytochrome d in the wild-type, and the expression of cydAB-lacZ, in the mutant, increased as the O2 supply was raised, suggesting that O2 regulates cydAB expression even in the absence of diazotrophy. In a strain carrying a mutation in cydR, a regulatory gene upstream of cydAB, and which over-produces cytochrome bd, the responses to O2 supply during growth at different O2 supply rates were reversed. Changes in the content of a haemoprotein detectable in low temperature photodissociation spectra, and attributed to cytochrome b 595 -the high-spin cytochrome b component of the cytochrome bd complex - followed the changes in cytochrome d levels. CO difference spectra of both the wild-type strain and the cytochrome bd-deficient mutant revealed a haemoprotein with spectral characteristics similar to cytochrome o, the levels of which increased as the O2 supply was raised. These results are discussed with reference to previous reports of cytochrome changes in cells grown under N2-fixing conditions

Therapeutic Targeting of the Tumour Microenvironment in Metastatic Colorectal Cancer

Liver metastasis is the primary contributor to the death of patients with colorectal cancer. Despite the overall success of current treatments including targeted therapy, chemotherapy, and immunotherapy combinations in colorectal cancer patients, the prognosis of patients with liver metastasis remains poor. Recent studies have highlighted the importance of the tumour microenvironment and the crosstalk within that determines the fate of circulating tumour cells in distant organs. Understanding the interactions between liver resident cells and tumour cells colonising the liver opens new therapeutic windows for the successful treatment of metastatic colorectal cancer. Here we discuss critical cellular interactions within the tumour microenvironment in primary tumours and in liver metastases that highlight potential therapeutic targets. We also discuss recent therapeutic advances for the treatment of metastatic colorectal cancer

Correlates of tobacco use and disease burden - an analysis of the World Health Organisation South East Asian Countries

Background World Health Organisation´s South-East Asia Region is home to about one fourth of the world's population. Of this, there are about 246 million smokers and 290 million smokeless tobacco users. In addition to the human toll, the effects of tobacco are felt significantly in the economy of a country. The World Bank has declared that, when effects of poverty due to loss of income, spending on tobacco and costs of illness are taken into account, tobacco use is a net loss to an economy. Methods Objective of the study: To determine the correlation of tobacco use with tobacco related disease burden and developmental indicators of South East Asian countries. Study method: Secondary data-based Cross-Sectional Study Data sources: Data on tobacco prevalence, cancer prevalence, human development index, tobacco economics and poverty indicators during the year 2014-2015 were collected from various sources such as WHO Report on Global Tobacco Epidemic 2015, World Bank data, Tobacco Atlas and World Life Expectancy 2014 reports. Data analysis: Data were analyzed using Pearsonn´s Correlation Coefficient on SPSS version 16. Results were considered significant at 95% level of significance. Results The Study represented data of 11 Countries of the WHO South East Asia Region. Current smoking prevalence ranged between 2.80% and 39.80% with a mean prevalence of 20.7% (± 9.24). Both prevalence of smoking and number of cigarettes smoked per year per ca-pita in the 11 countries had a positive moderate correlation with the number of deaths per 100000 population due to lung cancer (r= 0.62 &amp; 0.76; P=0.04 &amp; 0.01). Oral Cancer increased with prevalence of smokeless tobacco use (r=0.07; P=0.07). [Correlates between tobacco use and tobacco burden] Conclusions The study findings suggest that tobacco prevalence, tobacco related deaths and tobacco economics can be correlated with the health profile of countries in the region. These countries show positive correlation between tobacco use and related diseases

An in vitro comparison of resistance to second and third order archwire activations of three different varieties of esthetic brackets

Background: When ceramic brackets were introduced as an esthetic alternative to the stainless steel brackets, it was a step ahead in the use of esthetic appliances for orthodontic treatment. Although ceramic brackets had overcome the drawbacks of the initial polycarbonate brackets such as staining and bracket slot distortion, they posed an altogether different problem. This was on account of the physical properties of the ceramic material, namely brittleness. Purpose of the Study: The purpose of this study is to investigate the physical properties of three different varieties of esthetic brackets, i.e., "MXI" ceramic bracket, "Spirit MB" bracket (Ormco), a composite bracket with metal slot reinforcement and the third one fiber bracket, with silicious copolymer, Natura (Leone Co.). Aims and Objective of the Study : The objective of this study is to compare the torsional resistance of the brackets and resistance of brackets to second order archwire activations. Conclusion: The strength of the selected brackets was more than sufficient to withstand orthodontic load without any fracture or deformation