13 research outputs found
Efficient algorithms for reconstructing gene content by co-evolution
<p>Abstract</p> <p>Background</p> <p>In a previous study we demonstrated that co-evolutionary information can be utilized for improving the accuracy of ancestral gene content reconstruction. To this end, we defined a new computational problem, the Ancestral Co-Evolutionary (ACE) problem, and developed algorithms for solving it.</p> <p>Results</p> <p>In the current paper we generalize our previous study in various ways. First, we describe new efficient computational approaches for solving the ACE problem. The new approaches are based on reductions to classical methods such as linear programming relaxation, quadratic programming, and min-cut. Second, we report new computational hardness results related to the ACE, including practical cases where it can be solved in polynomial time.</p> <p>Third, we generalize the ACE problem and demonstrate how our approach can be used for inferring parts of the genomes of <it>non-ancestral</it> organisms. To this end, we describe a heuristic for finding the portion of the genome ('dominant set’) that can be used to reconstruct the rest of the genome with the lowest error rate. This heuristic utilizes both evolutionary information and co-evolutionary information.</p> <p>We implemented these algorithms on a large input of the ACE problem (95 unicellular organisms, 4,873 protein families, and 10, 576 of co-evolutionary relations), demonstrating that some of these algorithms can outperform the algorithm used in our previous study. In addition, we show that based on our approach a ’dominant set’ cab be used reconstruct a major fraction of a genome (up to 79%) with relatively low error-rate (<it>e.g.</it> 0.11). We find that the ’dominant set’ tends to include metabolic and regulatory genes, with high evolutionary rate, and low protein abundance and number of protein-protein interactions.</p> <p>Conclusions</p> <p>The <it>ACE</it> problem can be efficiently extended for inferring the genomes of organisms that exist today. In addition, it may be solved in polynomial time in many practical cases. Metabolic and regulatory genes were found to be the most important groups of genes necessary for reconstructing gene content of an organism based on other related genomes.</p
Visita de Enfermagem e dúvidas manifestadas pela família em unidade de terapia intensiva
Adaptive Radiation in Mediterranean Cistus (Cistaceae)
lineage consists of
12 species primarily distributed in Mediterranean habitats and
is herein subject to analysis. lineages), which display asymmetric
characteristics: number of species (2 vs. 10), leaf morphologies
(linear vs. linear to ovate), floral characteristics (small,
three-sepalled vs. small to large, three- or five-sepalled
flowers) and ecological attributes (low-land vs. low-land to
mountain environments). A positive phenotype-environment
correlation has been detected by historical reconstructions of
morphological traits (leaf shape, leaf labdanum content and leaf
pubescence). Ecological evidence indicates that modifications of
leaf shape and size, coupled with differences in labdanum
secretion and pubescence density, appear to be related to
success of new species in different Mediterranean habitats.