51 research outputs found
Syncytiotrophoblast Microvesicles Released from Pre-Eclampsia Placentae Exhibit Increased Tissue Factor Activity
Background: Pre-eclampsia is a complication of pregnancy associated with activation of coagulation. It is caused by the placenta, which sheds increased amounts of syncytiotrophoblast microvesicles (STBM) into the maternal circulation. We hypothesized that STBM could contribute to the haemostatic activation observed in pre-eclampsia. Methodology/Principal Findings: STBM were collected by perfusion of the maternal side of placentae from healthy pregnant women and women with pre-eclampsia at caesarean section. Calibrated automated thrombography was used to assess thrombin generation triggered by STBM-borne tissue factor in platelet poor plasma (PPP). No thrombin was detected in PPP alone but the addition of STBM initiated thrombin generation in 14/16 cases. Pre-eclampsia STBM significantly shortened the lag time (LagT, P = 0.01) and time to peak thrombin generation (TTP, P = 0.005) when compared to normal STBM. Blockade of tissue factor eliminated thrombin generation, while inhibition of tissue factor pathway inhibitor significantly shortened LagT (p = 0.01) and TTP (P,0.0001), with a concomitant increase in endogenous thrombin potential. Conclusions/Significance: STBM triggered thrombin generation in normal plasma in a tissue factor dependent manner, indicating that TF activity is expressed by STBM. This is more pronounced in STBM shed from pre-eclampsia placentae. As more STBM are shed in pre-eclampsia these observations give insight into the disordered haemostasis observed in thi
Relationship of serum and tumor levels of iron and iron-binding proteins to lymphocyte immunity against tumor antigen in breast cancer patients
Thyroid gland development in rachycentron canadum during early life stages
The aim of this study was to describe the ontogeny of thyroid follicles in cobia Rachycentron canadum. Larvae were sampled daily (n=15 - 20) from hatching until 15 dah (days after hatching). Following, larvae were sampled every two days by 28 dah; a new sample was taken at 53 dah. The samples were dehydrated, embedded in Paraplast, and sections of 3 µm were dewaxed, rehydrated and stained with HE and PAS. A single follicle was already present 1 dah and three follicles were found 8 dah. The number of follicles increased up to 19 on 53 dah. The diameter of follicles and follicular cell height were lower 1 dah (6.83 ± 1.00 and 4.6 ± 0.01 µm), but increased from 8 dah (24.03 ± 0.46 µm e 6.43 ± 0.46 µm). From 8 dah, the presence of reabsorption vesicles was observed in the colloid and from the 19 dah some follicles did not present colloid. The early thyroid follicle appearance in cobia larvae as well as the high quantity of follicles without colloid and/or with vesicles even after the metamorphosis, might be the explanation of the fast growth of the cobia.O objetivo deste estudo foi descrever a ontogenia dos folĂculos da tireĂłide em Rachycentron canadum. Larvas foram coletadas diariamente (n= 15 – 20) desde a eclosĂŁo atĂ© 15 dae (dias apĂłs eclosĂŁo). Posteriormente foram coletadas a cada dois dias atĂ© o 28 dae; uma nova amostragem ocorreu aos 53 dae. As larvas foram desidratadas e emblocadas em Paraplast e secções de 3 µm foram desparafinadas, reidratas e coradas com HE e PAS. Um folĂculo estava presente ao 1 dae e trĂŞs foram encontrados aos 8 dae. O nĂşmero de folĂculos aumentou atĂ© 19 aos 53 dae. O diâmetro dos folĂculos e a altura das cĂ©lulas foliculares foram menores ao 1dae (68,3 ± 1,00 e 4,6 ± 0,01 µm), mas aumentou a partir do 8 dae (24,03 ± 0,46 µm e 6,43 ± 0,46 µm). A partir do 8 dae a presença de vesĂculas de reabsorção foi observada no colĂłide e a partir de 19 dae alguns folĂculos nĂŁo apresentaram colĂłide. O surgimento precoce do folĂculo da tireĂłide no bijupirá assim como a grande quantidade de folĂculos sem colĂłide e/ou com a presença de vesĂculas mesmo apĂłs a metamorfose podem ser a explicação do rápido crescimento da espĂ©cie
Tumor regression with regional distribution of the targeted toxin TF-CRM107 in patients with malignant brain tumors
Regional patterns of extreme precipitation on Titan consistent with observed alluvial fan distribution
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