2,942 research outputs found
A robust seeding technique for the growth of single grain (RE)BCO and (RE)BCO-Ag bulk superconductors
Bulk, single grains of RE-Ba-Cu-O [(RE)BCO] high temperature superconductors have significant potential for a wide range of applications, including trapped field magnets, energy storage flywheels, superconducting mixers and magnetic separators. One of the main challenges in the production of these materials by the so-called top-seeded melt growth (TSMG) technique is the reliable seeding of large, single grains, which are required for high field applications. A chemically aggressive liquid phase comprising of BaCuO2 and CuO is generated during the single grain growth process, which comes into direct contact with the seed crystal either instantaneously or via infiltration through a buffer pellet, if employed in the process. This can cause either partial or complete melting of the seed, leading subsequently to growth failure. Here, the underlying mechanisms of seed crystal melting and the role of seed porosity in the single grain growth process are investigated. We identify seed porosity as a key limitation in the reliable and successful fabrication of large grain (RE)BCO bulk superconductors for the first time, and propose the use of Mg-doped NdBCO generic seeds fabricated via the infiltration growth (IG) technique to reduce the effects of seed porosity on the melt growth process. Finally, we demonstrate that the use of such seeds leads to better resistance to melting during the single grain growth process, and therefore to a more reliable fabrication technique
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Multiple seeding for the growth of bulk GdBCO-Ag superconductors with single grain behaviour
Rare earth–barium–copper oxide bulk superconductors fabricated in large or complicated geometries are required for a variety of engineering applications. Initiating crystal growth from multiple seeds reduces the time taken to melt-process individual samples and can reduce the problem of poor crystal texture away from the seed. Grain boundaries between regions of independent crystal growth can reduce significantly the flow of current due to crystallographic misalignment and the agglomeration of impurity phases. Enhanced supercurrent flow at such boundaries has been achieved by minimising the depth of the boundary between growth sectors generated during the melt growth process by reducing second phase agglomerations and by a new technique for initiating crystal growth that minimises the misalignment between different growth regions. The trapped magnetic fields measured for the resulting samples exhibit a single trapped field peak indicating they are equivalent to conventional single grains.The authors acknowledge support from the Engineering and Physical Sciences Research Council EP/K02910X/1.This is the final version of the article. It first appeared from the Institute of Physics via 10.1088/0953-2048/30/1/01500
Combined prokaryotic–eukaryotic delivery and expression of therapeutic factors through a primed autocatalytic positive-feedback loop
Progress in bacterial therapy for cancer and infectious diseases is hampered by the absence of safe and efficient vectors. Sustained delivery and high gene expression levels are critical for the therapeutic efficacy. Here we developed a Salmonella typhimrium strain to maintain and safely deliver a plasmid vector to target tissues. This vector is designed to allow dual transcription of therapeutic factors, such as cytotoxic proteins, short hairpin RNAs or combinations, in the nucleus or cytoplasm of eukaryotic cells, with this expression sustained by an autocatalytic positive-feedback loop. Mechanisms to prime the system and maintain the plasmid in the bacterium are also provided. Synergistic effects of attenuated Salmonella and our inter-kingdom system allow the precise expression of Diphtheria toxin A chain (DTA) gene in tumor microenvironment and eradicate large established tumors in immunocompetent animals. In the experiments reported here, 26% of mice (n = 5/19) with aggressive tumors were cured and the others all survived until the end of the experiment. We also demonstrated that ST4 packaged with shRNA-encoding plasmids has sustained knockdown effects in nude mice bearing human MDA-MB-231 xenografts. Three weeks after injection of 5 × 106 ST4/pIKT-shPlk, PLK1 transcript levels in tumors were 62.5 ± 18.6% lower than the vector control group (P = 0.015). The presence of PLK1 5′ RACE-PCR cleavage products confirmed a sustained RNAi-mediated mechanism of action. This innovative technology provides an effective and versatile vehicle for efficient inter-kingdom gene delivery that can be applied to cancer therapy and other purposes.postprin
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The processing and properties of bulk (RE)BCO high temperature superconductors: Current status and future perspectives
Funder: Engineering and Physical Sciences Research Council; doi: http://dx.doi.org/10.13039/501100000266Funder: King Abdulaziz City for Science and Technology; doi: http://dx.doi.org/10.13039/501100004919Bulk (RE)‒Ba‒Cu‒O [(RE)BCO] cuprate high temperature superconductors (HTS) have been developed steadily towards a wide range of sustainable engineering and technological applications since their discovery in 1986 based primarily on their unique potential to trap very large magnetic fields (> 5 T) at temperatures that are accessible potentially by thermo-electric cooling techniques. This paper reviews the current state of the art of the processing of large, single grain (RE)BCO bulk superconductors required to trap fields of this magnitude, and specifically via two advanced fabrication approaches; the traditional top-seeded melt growth (TSMG) process and the more recently developed top-seeded infiltration growth (TSIG) technique. The focus of the review is on optimising the critical processing parameters to achieve high-quality, high performance single grain (RE)BCO bulk superconductors specifically for high-field applications. The review also summarises recent advances in processing, such as the integration of the so-called buffer technique into the TSMG and TSIG processing methodologies to achieve improved reliability in single grain growth with a success rate exceeding 90%, the development of a Mg-doped NdBCO generic seed crystal for the successful growth of all rare-earth and light-rare earth based bulk superconductors [(RE)BCO and (LRE)BCO] and the introduction of nano-size stable, non-superconducting phase(s) to the bulk microstructure to improve the intrinsic flux pinning strength of the material, and hence trapped magnetic field. Details of the 2-step buffer-aided TSIG technique developed recently that yields dense, near-net shaped, high performance (RE)BCO bulk superconductors with improved superconducting and mechanical properties are also presented. Suitable sample-seed configurations for effective multi-seeding are discussed, which enables the production of high aspect ratio, bar-shaped (RE)BCO quasi-single grains that exhibit improved levitation forces required in Maglev-based applications, for example, are discussed. The electrical, mechanical, microstructural and magnetic properties (including those achieved from a pulsed-field magnetisation approach) of the different (RE)BCO systems are presented and the relevant correlation in properties and performance highlighted, accordingly. Finally, a brief summary of existing applications and prospects for near-future exploitation of these remarkable, technologically important materials, and particularly in the medical and pharma-industries, is provided.KACS
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Trapped magnetic field distribution above two magnetized bulk superconductors close to each other
Bulk large-grain superconductors can be used as high-field permanent magnets. Although the properties of such individual trapped field magnets are well documented, much less is known concerning their behaviour when two are brought together. In this work, the interaction between two cylindrical bulk YBa2Cu3O7 (YBCO) superconductors is described. Two sets of experiments were carried out. The first involved the simultaneous magnetization of two bulk superconductors placed a short distance apart. Here, the applied magnetic field was aligned parallel to the c-axis of one bulk, while the other was oriented with its c-axis offset . For a centre-to-centre distance equal to twice the sample height, the presence of the second sample is found not to alter the current distribution inside the first. Consequently, the contribution of both samples simply sums, thus increasing the magnetic flux density between them. In the second set of experiments, the translational approach of the superconductors with parallel c-axes was investigated. The following configurations were considered: (i) face to face approach (with anti-parallel trapped field orientation) and (ii) sideways approach (with parallel trapped field orientation). An irreversible decrease of the trapped field was measured on separation . Repeated approach cycles showed that the irreversible loss of trapped field is largest for the first approach.Henry Royce Institute (Equipment grant ref. EP/P024947/1)
We thank the University of Liege for equipment and travel grants. Michel Houbart is recipient of a FRS-FNRS Research Fellow gran
Redox linked flavin sites in extracellular decaheme proteins involved in microbe-mineral electron transfer
Extracellular microbe-mineral electron transfer is a major driving force for the oxidation of organic carbon in many subsurface environments. Extracellular multi-heme cytochromes of the Shewenella genus play a major role in this process but the mechanism of electron exchange at the interface between cytochrome and acceptor is widely debated. The 1.8 Ă… x-ray crystal structure of the decaheme MtrC revealed a highly conserved CX8C disulfide that, when substituted for AX8A, severely compromised the ability of S. oneidensis to grow under aerobic conditions. Reductive cleavage of the disulfide in the presence of flavin mononucleotide (FMN) resulted in the reversible formation of a stable flavocytochrome. Similar results were also observed with other decaheme cytochromes, OmcA, MtrF and UndA. The data suggest that these decaheme cytochromes can transition between highly reactive flavocytochromes or less reactive cytochromes, and that this transition is controlled by a redox active disulfide that responds to the presence of oxygen
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Composite stacks for reliable > 17 T trapped fields in bulk superconductor magnets
Trapped fields of over 20 T are, in principle, achievable in bulk,
single-grain high temperature cuprate superconductors. The principle barriers
to realizing such performance are, firstly, the large tensile stresses that
develop during the magnetization of such trapped-field magnets as a result of
the Lorentz force, which lead to brittle fracture of these ceramic-like
materials at high fields and, secondly, catastrophic thermal instabilities as a
result of flux movement during magnetization. Moreover, for a batch of samples
nominally fabricated identically, the statistical nature of the failure
mechanism means the best performance (i.e. trapped fields of over 17 T) cannot
be attained reliably. The magnetization process, particularly to higher fields,
also often damages the samples such that they cannot repeatedly trap high
fields following subsequent magnetization. In this study, we report the
sequential trapping of magnetic fields of ~ 17 T, achieving 16.8 T at 26 K
initially and 17.6 T at 22.5 K subsequently, in a stack of two Ag-doped
GdBa2Cu3O7-{\delta} bulk superconductor composites of diameter 24 mm reinforced
with (1) stainless-steel laminations, and (2) shrink-fit stainless steel rings.
A trapped field of 17.6 T is, in fact, comparable with the highest trapped
fields reported to date for bulk superconducting magnets of any mechanical and
chemical composition, and this was achieved using the first composite stack to
be fabricated by this technique
Factors Affecting the Growth of Multiseeded Superconducting Single Grains
© 2016 American Chemical Society.Single grain, rare earth-barium-copper oxide [(RE)BCO] bulk superconductors, fabricated either individually or assembled in large or complicated geometries, have a significant potential for a variety of potential engineering applications. Unfortunately, (RE)BCO single grains have intrinsically very low growth rates, which limits the sample size that may be achieved in a practical, top seeded melt growth process. As a result, a melt process based on the use of two or more seeds (so-called multiseeding) to control the nucleation and subsequent growth of bulk (RE)BCO superconductors has been developed to fabricate larger samples and to reduce the time taken for the melt process. However, the formation of regions that contain non-superconducting phases at grain boundaries has emerged as an unavoidable consequence of this process. This leads to the multiseeded sample behaving as if it is composed of multiple, singly seeded regions. In this work we have examined the factors that lead to the accumulation of non-superconducting phases at grain boundaries in multiseeded (RE)BCO bulk samples. We have studied the microstructure and superconducting properties of a number of samples fabricated by the multiseeded process to explore how the severity of this problem can be reduced significantly, if not eliminated completely. We conclude that, by employing the techniques described, multiseeding is a practical approach to the processing of large high performance superconducting bulk samples for engineering applications.Engineering and Physical Sciences Research Council (Grant ID: EP/K02910X/1
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