2,559 research outputs found
Quantum Geometry and Diffusion
We study the diffusion equation in two-dimensional quantum gravity, and show
that the spectral dimension is two despite the fact that the intrinsic
Hausdorff dimension of the ensemble of two-dimensional geometries is very
different from two. We determine the scaling properties of the quantum gravity
averaged diffusion kernel.Comment: latex2e, 10 pages, 4 figure
Techniques for analysing pattern formation in populations of stem cells and their progeny
<p>Abstract</p> <p>Background</p> <p>To investigate how patterns of cell differentiation are related to underlying intra- and inter-cellular signalling pathways, we use a stochastic individual-based model to simulate pattern formation when stem cells and their progeny are cultured as a monolayer. We assume that the fate of an individual cell is regulated by the signals it receives from neighbouring cells via either diffusive or juxtacrine signalling. We analyse simulated patterns using two different spatial statistical measures that are suited to planar multicellular systems: pair correlation functions (PCFs) and quadrat histograms (QHs).</p> <p>Results</p> <p>With a diffusive signalling mechanism, pattern size (revealed by PCFs) is determined by both morphogen decay rate and a sensitivity parameter that determines the degree to which morphogen biases differentiation; high sensitivity and slow decay give rise to large-scale patterns. In contrast, with juxtacrine signalling, high sensitivity produces well-defined patterns over shorter lengthscales. QHs are simpler to compute than PCFs and allow us to distinguish between random differentiation at low sensitivities and patterned states generated at higher sensitivities.</p> <p>Conclusions</p> <p>PCFs and QHs together provide an effective means of characterising emergent patterns of differentiation in planar multicellular aggregates.</p
Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing.
Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in many body fluids such as blood, breast milk and cerebrospinal fluid. However, there are conflicting results regarding the nature of exRNA. Here, we have separated 2 distinct exRNA profiles released by mast cells, here termed high-density (HD) and low-density (LD) exRNA. The exRNA in both fractions was characterized by microarray and next-generation sequencing. Both exRNA fractions contained mRNA and miRNA, and the mRNAs in the LD exRNA correlated closely with the cellular mRNA, whereas the HD mRNA did not. Furthermore, the HD exRNA was enriched in lincRNA, antisense RNA, vault RNA, snoRNA, and snRNA with little or no evidence of full-length 18S and 28S rRNA. The LD exRNA was enriched in mitochondrial rRNA, mitochondrial tRNA, tRNA, piRNA, Y RNA, and full-length 18S and 28S rRNA. The proteomes of the HD and LD exRNA-containing fractions were determined with LC-MS/MS and analyzed with Gene Ontology term finder, which showed that both proteomes were associated with the term extracellular vesicles and electron microscopy suggests that at least a part of the exRNA is associated with exosome-like extracellular vesicles. Additionally, the proteins in the HD fractions tended to be associated with the nucleus and ribosomes, whereas the LD fraction proteome tended to be associated with the mitochondrion.We show that the 2 exRNA signatures released by a single cell type can be separated by floatation on a density gradient. These results show that cells can release multiple types of exRNA with substantial differences in RNA species content. This is important for any future studies determining the nature and function of exRNA released from different cells under different conditions.112212Ysciescopu
Holographic two dimensional QCD and Chern-Simons term
We present a holographic realization of large Nc massless QCD in two
dimensions using a D2/D8 brane construction. The flavor axial anomaly is dual
to a three dimensional Chern-Simons term which turns out to be of leading
order, and it affects the meson spectrum and holographic renormalization in
crucial ways. The massless flavor bosons that exist in the spectrum are found
to decouple from the heavier mesons, in agreement with the general lore of
non-Abelian bosonization. We also show that an external dynamical photon
acquires a mass through the three dimensional Chern-Simons term as expected
from the Schwinger mechanism. Massless two dimensional QCD at large Nc exhibits
anti-vector-meson dominance due to the axial anomaly.Comment: 22 page
Bias in MRI Measurements of Apparent Diffusion Coefficient and Kurtosis: Implications for Choice of Maximum Diffusion Encoding
Tissue water diffusion is non-Gaussian and the expressions used to calculate diffusion parameters are approximations which introduce systematic errors dependent on the maximum diffusion encoding, diffusion time, etc. This study aimed at characterizing biases in estimates of both apparent diffusion coefficient and kurtosis, and determines their dependence on these parameters. Similar to the approach of several previous studies, Taylor expansion of the diffusion signal was used to calculate biases. Predicted errors were compared with data from one volunteer. Predicted errors agreed well with the measured errors and also the published diffusion tensor imaging measurements. The equations derived predict biases in measured diffusion parameters and explain much of the discrepancy between measurements obtained with different acquisition protocols. The equations may also be used to choose appropriate diffusion encoding for diffusion weighted, tensor, and kurtosis imaging
Holographic chiral magnetic spiral
We study the ground state of baryonic/axial matter at zero temperature
chiral-symmetry broken phase under a large magnetic field, in the framework of
holographic QCD by Sakai-Sugimoto. Our study is motivated by a recent proposal
of chiral magnetic spiral phase that has been argued to be favored against
previously studied phase of homogeneous distribution of axial/baryonic currents
in terms of meson super-currents dictated by triangle anomalies in QCD. Our
results provide an existence proof of chiral magnetic spiral in strong coupling
regime via holography, at least for large axial chemical potentials, whereas we
don't find the phenomenon in the case of purely baryonic chemical potential.Comment: 24 pages, 15 figure
TGF-b2 induction regulates invasiveness of theileria-transformed leukocytes and disease susceptibility
Theileria parasites invade and transform bovine leukocytes causing either East Coast fever (T. parva), or tropical theileriosis (T. annulata). Susceptible animals usually die within weeks of infection, but indigenous infected cattle show markedly reduced pathology, suggesting that host genetic factors may cause disease susceptibility. Attenuated live vaccines are widely used to control tropical theileriosis and attenuation is associated with reduced invasiveness of infected macrophages in vitro. Disease pathogenesis is therefore linked to aggressive invasiveness, rather than uncontrolled proliferation of Theileria-infected leukocytes. We show that the invasive potential of Theileria-transformed leukocytes involves TGF-b signalling. Attenuated live vaccine lines express reduced TGF-b2 and their invasiveness can be rescued with exogenous TGF-b. Importantly, infected macrophages from disease susceptible Holstein-Friesian (HF) cows express more TGF-b2 and traverse Matrigel with great efficiency compared to those from disease-resistant Sahiwal cattle. Thus, TGF-b2 levels correlate with disease susceptibility. Using fluorescence and time-lapse video microscopy we show that Theileria-infected, disease-susceptible HF macrophages exhibit increased actin dynamics in their lamellipodia and podosomal adhesion structures and develop more membrane blebs. TGF-b2-associated invasiveness in HF macrophages has a transcription-independent element that relies on cytoskeleton remodelling via activation of Rho kinase (ROCK). We propose that a TGF-b autocrine loop confers an amoeboid-like motility on Theileria-infected leukocytes, which combines with MMP-dependent motility to drive invasiveness and virulence
Recovery of gastric evacuation rate in Atlantic cod Gadus morhua L. surgically implanted with a dummy telemetry devic
D3/D7 Quark-Gluon Plasma with Magnetically Induced Anisotropy
We study the effects of the temperature and of a magnetic field in the setup
of an intersection of D3/D7 branes, where a large number of D7 branes is
smeared in the transverse directions to allow for a perturbative solution in a
backreaction parameter. The magnetic field sources an anisotropy in the plasma,
and we investigate its physical consequences for the thermodynamics and energy
loss of particles probing the system. In particular we comment on the
stress-energy tensor of the plasma, the propagation of sound in the directions
parallel and orthogonal to the magnetic field, the drag force of a quark moving
through the medium and jet quenching.Comment: 29 pages + appendices, 5 figures. v2 Version to appear in JHEP, with
minor revisions, references added and typos correcte
Recurrence of Diabetic Pedal Ulcerations Following Tendo-Achilles Lengthening
Foot and ankle surgeons are frequently challenged by the devastating systemic consequences of diabetes mellitus manifested through neuropathy, integumentary and joint breakdown, delayed healing, decreased ability to fight infection, and fragile tendon/ligaments. Diabetic neuropathic pedal ulcerations lead to amputations at an alarming rate and also carry a high mortality rate. This article will discuss causes of diabetic pedal ulcerations that persist or recur after tendo-Achilles lengthening and will highlight areas that need to be addressed by the practitioner such as infection, vascular and nutritional status, glucose control, off-loading, biomechanics, and patient compliance
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