Benthic foraminifers and siliceous sponge spicules assemblages in the Quaternary rhodolith rich sediments from Pontine Archipelago shelf

The bottom samples (Quaternary in age) of two cores (CS1 and Caro1) collected at 60 and 122 m water depth in the marine area near Ponza Island (Pontine Archipelago, Tyrrhenian Sea) are investigated. In particular, benthic foraminifers and siliceous sponge spicules are considered. The coralline red algae (pralines, boxworks and unattached branches) are abundant in both samples and, particularly, in the CS1 bottom as well as the benthic foraminifers. The siliceous sponge spicules also are very diversified and abundant in the CS1 bottom sample, while in the Caro1 bottom they are rare and fragmented. Benthic foraminiferal assemblage of two samples is dominated by Asterigerinata mamilla and Lobatula lobatula, typical epiphytic species but also able to live on circalittoral detrital seafloors, adapting to an epifaunal lifestyle. Based on these data the bottom of the studied cores represents the upper circalittoral zone, within the present-day depth limit distribution of coralline red algae in the Pontine Archipelago (shallower than 100 m water depth)

Analysis of ring laser gyroscopes including laser dynamics

Inertial sensors stimulate very large interest, not only for their application but also for fundamental physics tests. Ring laser gyros, which measure angular rotation rate, are certainly among the most sensitive inertial sensors, with excellent dynamic range and bandwidth. Large area ring laser gyros are routinely able to measure fractions of prad/s, with high duty cycle and bandwidth, providing fast, direct and local measurement of relevant geodetic and geophysical signals. Improvements of a factor $10-100$ would open the windows for general relativity tests, as the GINGER project, an Earth based experiment aiming at the Lense-Thirring test at $1\%$ level. However, it is well known that the dynamics of the laser induces non-linearities, and those effects are more evident in small scale instruments. Sensitivity and accuracy improvements are always worthwhile, and in general there is demand for high sensitivity environmental study and development of inertial platforms, where small scale transportable instruments should be used. We discuss a novel technique to analyse the data, aiming at studying and removing those non-linearity. The analysis is applied to the two ring laser prototypes GP2 and GINGERINO, and angular rotation rate evaluated with the new and standard methods are compared. The improvement is evident, it shows that the back-scatter problem of the ring laser gyros is negligible with a proper analysis of the data, improving the performances of large scale ring laser gyros, but also indicating that small scale instruments with sensitivity of nrad/s are feasible.Comment: 9 pages and 7 figure

Fc receptor-mediated phagocytosis occurs in macrophages at exceedingly low cytosolic Ca2+ levels.

Cytosolic free Ca2+ ([Ca2+]i) homeostasis was investigated in mouse peritoneal macrophages and in the macrophage-like cell line J774. [Ca2+]i measurements were performed in both cells in suspension and cells in monolayers loaded with either quin2 or fura-2. Resting [Ca2+]i was 110-140 and 85-120 nM for cell suspensions and monolayers, respectively. There were no significant differences in [Ca2+]i between the two macrophage populations whether quin2 or fura-2 were used as Ca2+ indicators. Addition of heat-aggregated IgG, IgG-coated erythrocyte ghosts, or a rat monoclonal antibody (2.4G2) directed against mouse Fc receptor II induced a rise in [Ca2+]i. This [Ca2+]i increase was consistently observed in J774 and peritoneal macrophage suspensions and in J774 macrophage monolayers; in contrast it was observed inconsistently in peritoneal macrophages in monolayer cultures. The increase in [Ca2+]i induced by ligation of Fc receptors was inhibited totally in macrophages in suspension and by 80% in macrophages in monolayers by a short preincubation of macrophages with PMA; however, phagocytosis itself was unaffected. The effect of reducing cytosolic Ca2+ to very low concentrations on Fc receptor-mediated phagocytosis was also investigated. By incubating macrophages with high concentrations of quin2/AM in the absence of extracellular Ca2+, or by loading EGTA into the cytoplasm, the [Ca2+]i was buffered and clamped to 1-10 nM. Despite this, the phagocytosis of IgG-coated erythrocytes proceeded normally. These observations confirm the report of Young et al. (Young, J. D., S. S. Ko, and Z. A. Cohn. 1984. Proc. Natl. Acad. Sci. USA. 81:5430-5434) that ligation of Fc receptors causes Ca2+ mobilization in macrophages. However, these results confirm and extend the findings of McNeil et al. (McNeil, P. L., J. A. Swanson, S. D. Wright, S. C. Silverstein, and D. L. Taylor. 1986. J. Cell Biol. 102:1586-1592) that a rise in [Ca2+]i is not required for Fc receptor-mediated phagocytosis; and they provide direct evidence that Fc receptor-mediated phagocytosis occurs normally even at exceedingly low [Ca2+]i

Extracellular ATP Induces a Distorted Maturation of Dendritic Cells and Inhibits Their Capacity to Initiate Th1 Responses

AbstractDendritic cells (DCs) express functional purinergic receptors, but the effects of purine nucleotides on DC functions have been marginally investigated. In this study, we report on the ability of micromolar concentrations of ATP to affect the maturation and Ag-presenting function of monocyte-derived DCs in vitro. Chronic stimulation (24 h) of DCs with low, noncytotoxic ATP doses increased membrane expression of CD54, CD80, CD86, and CD83, slightly reduced the endocytic activity of DCs, and augmented their capacity to promote proliferation of allogeneic naive T lymphocytes. Moreover, ATP enhanced LPS- and soluble CD40 ligand-induced CD54, CD86, and CD83 expression. On the other hand, ATP markedly and dose-dependently inhibited LPS- and soluble CD40 ligand-dependent production of IL-1α, IL-1β, TNF-α, IL-6, and IL-12, whereas IL-1 receptor antagonist and IL-10 production was not affected. As a result, T cell lines generated from allogeneic naive CD45RA+ T cells primed with DCs matured in the presence of ATP produced lower amounts of IFN-γ and higher levels of IL-4, IL-5, and IL-10 compared with T cell lines obtained with LPS-stimulated DCs. ATP inhibition of TNF-α and IL-12 production by mature DCs was not mediated by PGs or elevation of intracellular cAMP and did not require ATP degradation. The inability of UTP and the similar potency of ADP to reproduce ATP effects indicated that ATP could function through the P2X receptor family. These results suggest that extracellular ATP may serve as an important regulatory signal to dampen IL-12 production by DCs and thus prevent exaggerated and harmful immune responses

Ytterbium Disilicate/Monosilicate Multilayer Environmental Barrier Coatings: Influence of Atmospheric Plasma Spray Parameters on Composition and Microstructure

first_pagesettingsOrder Article Reprints Open AccessArticle Ytterbium Disilicate/Monosilicate Multilayer Environmental Barrier Coatings: Influence of Atmospheric Plasma Spray Parameters on Composition and Microstructure by Giulia Di Iorio,Laura Paglia *ORCID,Giulia PedrizzettiORCID,Virgilio GenovaORCID,Francesco MarraORCID,Cecilia BartuliORCID andGiovanni PulciORCID INSTM Reference Laboratory for Materials and Surface Engineering, Sapienza University of Rome, Eudossiana 18, 00184 Rome, Italy * Author to whom correspondence should be addressed. Coatings 2023, 13(9), 1602; https://doi.org/10.3390/coatings13091602 Original submission received: 10 August 2023 / Revised: 31 August 2023 / Accepted: 11 September 2023 / Published: 13 September 2023 Downloadkeyboard_arrow_down Browse Figures Review Reports Versions Notes Abstract SiC/SiC ceramic matrix composites (SiCf/SiC CMCs) are regarded as the new materials for the hot-section components of aircraft gas turbine engines, since they have one-third of the density of metallic superalloys, a higher temperature capability, good mechanical strength, and excellent thermal shock resistance. However, high-temperature water-vapor-rich combustion gases can induce severe surface recession phenomena in SiC/SiC leading to component failure. For this reason, it is necessary to design protective coatings, i.e., environmental barrier coatings (EBCs), able to protect the SiC/SiC surface in combustion environments. In the present work, ytterbium monosilicate (Yb2SiO5), stable when exposed to water vapor at high temperatures, and ytterbium disilicate (Yb2Si2O7), characterized by a thermal expansion coefficient closer to that of the substrate, were selected for a multilayer EBC system. EBCs were processed using the atmospheric plasma spray (APS) technique. A set of deposition parameters were tested, varying the power of the torch, and the composition and microstructure of the deposited coatings were studied in terms of porosity, crack density, and post-deposition phase retention by performing SEM, EDS, and XRD analysis. The results allow for the definition of the influence of deposition parameters on the final properties of multilayer EBC coatings

Benthic foraminiferal assemblages and rhodolith facies evolution in post-LGM sediments from the Pontine Archipelago shelf (Central Tyrrhenian Sea, Italy)

The seabed of the Pontine Archipelago (Tyrrhenian Sea) insular shelf is peculiar as it is characterized by a mixed siliciclastic–carbonate sedimentation. In order to reconstruct the Late Quaternary paleoenvironmental evolution of the Pontine Archipelago, this study investigates the succession of facies recorded by two sediment cores. For this purpose, benthic foraminifera and rhodoliths assemblages were considered. The two cores (post-Last Glacial Maximum in age) were collected at 60 (CS1) and 122 m (Caro1) depth on the insular shelf off Ponza Island. The paleontological data were compared with seismo-stratigraphic and lithological evidence. The cores show a deepening succession, with a transition from a basal rhodolith-rich biodetritic coarse sand to the surface coralline-barren silty sand. This transition is more evident along core Caro1 (from the bottom to the top), collected at a deeper water depth than CS1. In support of this evidence, along Caro1 was recorded a fairly constant increase in the amount of planktonic foraminiferal and a marked change in benthic foraminiferal assemblages (from Asterigerinata mamilla and Lobatula lobatula assemblage to Cassidulina carinata assemblage). Interestingly, the dating of the Caro1 bottom allowed us to extend to more than 13,000 years BP the rhodolith record in the Pontine Archipelago, indicating the possible presence of an active carbonate factory at that time

Functional and structural alterations in the endoplasmic reticulum and mitochondria during apoptosis triggered by C2-ceramide and??CD95/APO-1/FAS receptor stimulation

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