Technical considerations for the use of CRISPR/Cas9 in hematology research

The hematopoietic system is responsible for transporting oxygen and nutrients, fighting infections, and repairing tissue damage. Hematopoietic system dysfunction therefore causes a range of serious health consequences. Lifelong hematopoiesis is maintained by repopulating multipotent hematopoietic stem cells (HSCs) that replenish shorter-lived, mature blood cell types. A prokaryotic mechanism of immunity, the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 nuclease system, has been recently "repurposed" to mutate mammalian genomes efficiently and in a sequence-specific manner. The application of this genome-editing technology to hematology has afforded new approaches for functional genomics and even the prospect of "correcting" dysfunctional HSCs in the treatment of serious genetic hematological diseases. In this Perspective, we provide an overview of three recent CRISPR/Cas9 methods in hematology: gene disruption, gene targeting, and saturating mutagenesis. We also summarize the technical considerations and advice provided during the May 2017 International Society of Experimental Hematology New Investigator Committee webinar on the same topic

Um programa sobre promoção da saúde do adulto para trabalhadores em saúde Un programa sobre promoción de la salud del adulto para trabajadores en salud A program about adult health promotion for health workers

O objetivo desse trabalho é avaliar uma metodologia alternativa de educação em saúde do adulto desenvolvida entre trabalhadores em saúde. A avaliação foi feita através da pergunta orientadadora: O que significou para você o Curso de Saúde do adulto? A análise consistiu em destacar dos depoimentos as afirmações que mais se repetiam, e agrupá-las em sete categorias: 01. Aprender coisas novas; 02. Aprender para orientar as pessoas que freqüentam a Unidade Básica de Saúde; 03. Interação com os colegas; 04. Sentir-se valorizando profissionalmente; 05. Preocupação com os filhos; 06. Melhorar estilo de vida; 07. Sugestões. Os resultados remetem aos pressupostos apresentados na introdução do trabalho, bem como os do modelo epidemiológico proposto por BLUM4.<br>El objetivo de este trabajo es avaluar una metodologia alternativa de educación en salud del adulto desarrollada entre trabajadores de salud. La avaluación fué hecha a través de la pregunta orientadora: ¿Lo que ha significado para Ud. el curso Salud del Adulto? La análisis se ha constituído para destacar de las deposiciones, las afirmaciones que más se repetian, y agrupadas en siete categorias: 01. Aprender cosas nuevas; 02. Aprender para orientar las personas que frecuentan las Unidades Básicas de Salud; 03. Interacción entre los colegas; 04. Sentirse valorado en la profesión; 05. Preocupación con los hijos; 06. Mejor estilo de vida; 07. Sugestiones. Los resultados remeten a los presupuestos presentados en la introducción del trabajo así como los el modelo epidemiológico propuesto por BLUM4.<br>The porpoise of this work is to evaluate an alternative methodology of education in adult health care undertaken with health care workers. The evaluation was made by the directional question: what did the Adult Health Course mean to you? The analysis consisted of detecting in the responses, the affirmatives that were respective and grouping them in seven categories: 01. Learning new things; 02. Learning to orient people who goes to the basic Health Unit; 03. Interaction with colleagues; 04. Feeling professionally appraned; 05. Worrisome with sons; 06. Improving way of life; 07. Suggestions. The results to the presuppositions presented in the introduction of the article, as well as, with the Epidemiological Model proposed by BLUM4

Changes in the composition of cotton fibre cell walls during development

Purified cell walls, prepared from cotton fibres (Gossypium arboreum L.) at different growth stages, were subjected to successive extractions to give pectic, hemicellulosic, and agr-cellulosic fractions. The protein content and sugars obtained after hydrolysis of the total cell walls and of the various fractions were quantitatively estimated. The amount of protein in the fibre cell walls from one ovule reached a maximum value at the end of the elongation growth, decreased, and then reached a second maximum at the end of the secondary wall deposition. The absolute amounts of fucose, galactose, mannose, rhamnose, arabinose, uronic acid, and non-cellulosic glucose residues all reached a maximum at the end of the primary wall formation or at the beginning of the secondary wall formation. Only the absolute amounts of xylose and of the cellulosic glucose residues increased until the end of the fibre development. Most conspicuous was the decrease in the absolute amounts of non-cellulosic glucose and of arabinose residues during the secondary wall formation, possibly indicating a turnover of at least some of the hemicellulosic wall material