AglH, a thermophilic UDP‑<i>N</i>‑acetylglucosamine‑1‑phosphate:dolichyl phosphate GlcNAc‑1‑phosphotransferase initiating protein<i> N</i>‑glycosylation pathway in <i>Sulfolobus acidocaldarius</i>, is capable of complementing the eukaryal Alg7

AglH, a predicted UDP-GlcNAc-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase, is initiating the protein N-glycosylation pathway in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. AglH successfully replaced the endogenous GlcNAc-1-phosphotransferase activity of Alg7 in a conditional lethal Saccharomyces cerevisiae strain, in which the first step of the eukaryal protein N-glycosylation process was repressed. This study is one of the few examples of cross-domain complementation demonstrating a conserved polyprenyl phosphate transferase reaction within the eukaryal and archaeal domain like it was demonstrated for Methanococcus voltae (Shams-Eldin et al. 2008). The topology prediction and the alignment of the AglH membrane protein with GlcNAc-1-phosphotransferases from the three domains of life show significant conservation of amino acids within the different proposed cytoplasmic loops. Alanine mutations of selected conserved amino acids in the putative cytoplasmic loops II (D(100)), IV (F(220)) and V (F(264)) demonstrated the importance of these amino acids for cross-domain AlgH activity in in vitro complementation assays in S. cerevisiae. Furthermore, antibiotic treatment interfering directly with the activity of dolichyl phosphate GlcNAc-1-phosphotransferases confirmed the essentiality of N-glycosylation for cell survival

Cruise: YR121006, Stations: S5038- S5077, Clay Bank, York River Virginia 6-hour MUDBED Calibration Survey bracketing a Flood Tide

During each station in the survey a profile or bottom time series was collected with a suite of instrumentation including: a RDI 1200 KHz ADCP, a YSI 6600 CTD, a Sequia LISST 100X, a Sontek ADVOcean and a PICS floc camera. The raw data of profile stations are processed to provide a smooth profile of data throughout the water column and a series of between 2 to 5 minute bursts from various heights in the water column. Bottom bursts are time series collected when the profiler was resting on the seafloor. Total Suspended Solids (and fixed solids) were sampled from depth to calibrate the acoustic backscatter. The “logbook” is the hand written field notes and instrument setup documents. The “Consecutive Station Log” is an excel spreadsheet of the metadata associated with each station in the survey. Excel spreadsheet “Averaged Data” contains burst averaged data and statistics from the water column and bottom bursts. Raw and processed data from each instrument are zipped in a folder, or series of folders, identified by the type and serial number of the instrument. All times are Eastern Standard Time (EST).Dataset consists of profile and water column burst data and bottom burst data collected as part of a 6-hour anchor station survey in support of an Acoustic Doppler Velicometer (ADV) tripod deployed in nearby location