A Model for the Interplay of Receptor Recycling and Receptor-Mediated Contact in T Cells

Orientation of organelles inside T cells (TC) toward antigen-presenting cells (APC) ensures that the immune response is properly directed, but the orientation mechanisms remain largely unknown. Structural dynamics of TC are coupled to dynamics of T-cell receptor (TCR), which recognizes antigen on the APC surface. Engagement of the TCR triggers its internalization followed by delayed polarized recycling to the plasma membrane through the submembrane recycling compartment (RC), which organelle shares intracellular location with the TC effector apparatus. TCR engagement also triggers TC-APC interface expansion enabling further receptor engagement. To analyze the interplay of the cell-cell contact and receptor dynamics, we constructed a new numerical model. The new model displays the experimentally observed selective stabilization of the contact initiated next to the RC, and only transient formation of contact diametrically opposed to the RC. In the general case wherein the TC-APC contact is initiated in an arbitrary orientation to the RC, the modeling predicts that the contact dynamics and receptor recycling can interact, resulting effectively in migration of the contact to the TC surface domain adjacent to the submembrane RC. Using three-dimensional live-cell confocal microscopy, we obtain data consistent with this unexpected behavior. We conclude that a TC can stabilize its contact with an APC by aligning it with the polarized intracellular traffic of TCR. The results also suggest that the orientation of TC organelles, such as the RC and the effector apparatus, toward the APC can be achieved without any intracellular translocation of the organelles

Sucrose in the concentrated solution or the supercooled “state” : a review of caramelisation reactions and physical behaviour

Sucrose is probably one of the most studied molecules by food scientists, since it plays an important role as an ingredient or preserving agent in many formulations and technological processes. When sucrose is present in a product with a concentration near or greater than the saturation point—i.e. in the supercooled state—it possesses high potentialities for the food industry in areas as different as pastry industry, dairy and frozen desserts or films and coatings production. This paper presents a review on critical issues and research on highly concentrated sucrose solutions—mainly, on sucrose thermal degradation and relaxation behaviour in such solutions. The reviewed works allow identifying several issues with great potential for contributing to significant advances in Food Science and Technology.Authors are grateful for the valuable discussions with Teresa S. Brandao and Rosiane Lopes da Cunha during this research. Author M. A. C. Quintas acknowledges the financial support of her research by FCT grant SFRH/BPD/41715/2007

Generation of Up to Date Land Cover Maps for Central Asia

Human activity and climate variability has always changed the Earth’s surface and both will mainly contribute to future alteration in land cover and land use changes. In this chapte we demonstrate a land cover and land use classification approach for Central Asia addressing regional characteristics of the study area. With the aim of regional classification map for Central Asia a specific classification scheme based on the Land Cover Classification System (LCCS) of the Food and Agriculture Organisation of the United Nations Environment Programme (FAO-UNEP) was developed. The classification was performed by using a supervised classification method applied on metrics, which were derived from Moderate Resolution Imaging Spectroradiometer (MODIS) data with 250 m spatial resolution. The metrics wer derived from annual time-series of red and nearinfrared reflectance as well as from Normalized Difference Vegetation Index (NDVI) and thus reflect the temporal behavior of different land cover types. Reference data required for a supervised classification approach were collected from several high resolution satellite imagery distributed all over the study area. The overall accuracy results for performed classification of the year 2001 and 2009 are 91.2 and 91.3 %. The comparison of both classification maps shows significant alterations for different classes. Water bodies such as Shardara Water Reservoir and Aral Sea have changed in their extent. Whereby, the size of the Shardara Water Reservoir is very dynamic from year to year due to water management and the eastern lobe of southern Aral Sea has decreased because of the lack of inflow from Amu Darja. Furthermore, some large scale changes were detected in sparsely vegetated areas in Turkmenistan, where spring precipitation mainly affects the vegetation density. In the north of Kazakhstan significant forest losses caused by forest fires and logging were detected. The presented classification approach is a suitable tool for monitoring land cover and land use in Central Asia. Such independent information is important for accurate assessment of water and land recourses