Circulating tumor cells: Cancer's deadly couriers

10.1063/PT.3.2275Physics Today67226-30PHTO

DNA methylation of apoptosis genes in rectal cancer predicts patient survival and tumor recurrence

Development and application of statistical models for medical scientific researc

LINE-1 Hypomethylation Predicts Tumor Recurrence and Patient Survival in Early Stage Rectal Cancer

Surgical oncolog

Epigenetic status of LINE-1 predicts clinical outcome in early-stage rectal cancer

Surgical oncolog

Chromatin status of apoptosis genes correlates with sensitivity to chemo-, immune- and radiation therapy in colorectal cancer cell lines

Surgical oncolog

Assessment of DNA methylation status in early stages of breast cancer development

BACKGROUND: Molecular pathways determining the malignant potential of premalignant breast lesions remain unknown. In this study, alterations in DNA methylation levels were monitored during benign, premalignant and malignant stages of ductal breast cancer development. METHODS: To study epigenetic events during breast cancer development, four genomic biomarkers (Methylated-IN-Tumour (MINT)17, MINT31, RARβ2 and RASSF1A) shown to represent DNA hypermethylation in tumours were selected. Laser capture microdissection was employed to isolate DNA from breast lesions, including normal breast epithelia (n=52), ductal hyperplasia (n=23), atypical ductal hyperplasia (n=31), ductal carcinoma in situ (DCIS, n=95) and AJCC stage I invasive ductal carcinoma (IDC, n=34). Methylation Index (MI) for each biomarker was calculated based on methylated and unmethylated copy numbers measured by Absolute Quantitative Assessment Of Methylated Alleles (AQAMA). Trends in MI by developmental stage were analysed. RESULTS: Methylation levels increased significantly during the progressive stages of breast cancer development; P-values are 0.0012, 0.0003, 0.012, <0.0001 and <0.0001 for MINT17, MINT31, RARβ2, RASSF1A and combined biomarkers, respectively. In both DCIS and IDC, hypermethylation was associated with unfavourable characteristics. CONCLUSION: DNA hypermethylation of selected biomarkers occurs early in breast cancer development, and may present a predictor of malignant potential

Primary tumor classification according to methylation pattern is prognostic in patients with early stage ER-negative breast cancer

Development and application of statistical models for medical scientific researc

IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel

10.1038/ncomms9746Nature Communications6874

Hypomethylation of LINE-1 in primary tumor has poor prognosis in young breast cancer patients: a retrospective cohort study

Surgical oncolog