Pathogen infection influences a distinct microbial community composition in sorghum RILs

AIMS: The rhizosphere microbiome substantially affects plant health, yet comparatively little is known regarding the foliar community dynamics. Here, we examine the relationship between the microbiota and their response to natural infection by pathogens. METHODS: We established an experimental system using a set of sorghum recombinant inbred lines (RILS). These RILS included four models denoted as resistant, moderately resistant, susceptible and highly susceptible. A combination of 16S rRNA and ITS gene amplicon approaches was used to assess bacteria and fungi, respectively, in foliar samples. RESULTS: We show that the foliar microbiome differs substantially in asymptomatic and symptomatic RILs subsequent to natural infection by pathogens. A significant association was found between plant health and microbial community structure. Our analyses revealed several distinct fungal and bacterial pathogens. These pathogens included Gibberella and Pantoea genera, which were associated with the highly susceptible group. In addition to these pathogens, we also found signatures for Ascochyta, a known plant pathogenic genus. Members of the bacterial genus Methylorubrum and the fungal genus Hannaella, both known to exhibit plant growthpromoting (PGP) traits, were associated with the resistant and moderately resistant groups. These data also reveal numerous highly diverse fungal and bacterial taxa in RILs that did not show symptoms. We also found taxonomic differences between the microbiota hosted by the symptomatic and asymptomatic RILs. CONCLUSIONS: Together, these data suggest that pathogen infection may result in distinct microbiota. These results suggest that highly diverse microbiome may promote the plants ability to resist the effects of pathogens potentially contributing to plant health.The National Research Foundation, the Agricultural Research Council and the University of South Africa.http://link.springer.com/journal/11104pm2022BiochemistryGeneticsMicrobiology and Plant Patholog

Functional analysis and characterization of the human RBBP6 promoters based on a combination of molecular biology and in silico approaches provide additional evidence for RBBP6 role in apoptosis

Retinoblastoma Binding Protein 6 (RBBP6) has beenimplicated in the apoptotic pathway and regulation of the cellcycle through its interaction with p53, Rb and YB1 andubiquitination of YB1. Analysis of transcripts indicated theexistence of two promoters responsible for the regulation of thegene namely Promoter 0 (P0) and Promoter 1 (P1). With acombination of molecular and computational techniques weanalysed and functionally characterised these two promoters ofthe RBBP6 gene. In this study, we demonstrate that the activityof both promoters increase following camptothecin-inducedapoptosis, with notable difference in activities of P0 relative toP1, though activities of both have increased. Computationalanalysis suggests that both P0 and P1 transcription start sites arelocated within CpG islands, and that P1 has a functional TATAboxcontrary to P0. Promoter content analysis further suggestedthe presence of six-transcription factor binding sites that arecommon to both promoters and several others unique to eachpromoter. Functional analysis of all the transcription factorsusing GO, KEGG and DAVID analysis, associated P0 morestrongly with apoptosis, while P1 was associated more with theregulation of the cell cycle. In addition, P1 was also associatedwith ubiquitin-mediated proteolysis, while both promoters havebeen associated with the antigen processing and presentationpathway. Although an association between RBBP6 and cancerhas already been made, we also found additional associationswith diabetes and Alzheimer’s disease

Functional analysis and characterization of the human RBBP6 promoters based on a combination of molecular biology and in silico approaches provide additional evidence for RBBP6 role in apoptosis

Retinoblastoma Binding Protein 6 (RBBP6) has been implicated in the apoptotic pathway and regulation of the cell cycle through its interaction with p53, Rb and YB1 and ubiquitination of YB1. Analysis of transcripts indicated the existence of two promoters responsible for the regulation of the gene namely Promoter 0 (P0) and Promoter 1 (P1). With a combination of molecular and computational techniques we analysed and functionally characterised these two promoters of the RBBP6 gene. In this study, we demonstrate that the activity of both promoters increase following camptothecin-induced apoptosis, with notable difference in activities of P0 relative to P1, though activities of both have increased. Computational analysis suggests that both P0 and P1 transcription start sites are located within CpG islands, and that P1 has a functional TATAbox contrary to P0. Promoter content analysis further suggested the presence of six-transcription factor binding sites that are common to both promoters and several others unique to each promoter. Functional analysis of all the transcription factors using GO, KEGG and DAVID analysis, associated P0 more strongly with apoptosis, while P1 was associated more with the regulation of the cell cycle. In addition, P1 was also associated with ubiquitin-mediated proteolysis, while both promoters have been associated with the antigen processing and presentation pathway. Although an association between RBBP6 and cancer has already been made, we also found additional associations with diabetes and Alzheimer’s disease. - See more at: http://dl4.globalstf.org/?wpsc-product=functional-analysis-and-characterization-of-the-human-rbbp6-promoters-based-on-a-combination-of-molecular-biology-and-in-silico-approaches-provide-additional-evidence-for-rbbp6-role-in-apoptosis#more-231