3 research outputs found
Supplementary Material for: Application of a Global Proteomic Approach to Archival Precursor Lesions: Deleted in Malignant Brain Tumors 1 and Tissue Transglutaminase 2 Are Upregulated in Pancreatic Cancer Precursors
<p><i>Background:</i> Pancreatic cancer is an almost uniformly fatal
disease, and early detection is a critical determinant of improved
survival. A variety of noninvasive precursor lesions of pancreatic
adenocarcinoma have been identified, which provide a unique opportunity
for intervention prior to onset of invasive cancer. Biomarker discovery
in precursor lesions has been hampered by the ready availability of
fresh specimens, and limited yields of proteins suitable for large scale
screening. <i>Methods:</i> We utilized Liquid Tissue®, a novel
technique for protein extraction from archival formalin-fixed material,
and mass spectrometry to conduct a global proteomic analysis of an
intraductal papillary mucinous neoplasm (IPMN). Tissue microarrays
comprised of 38 IPMNs were used for validation of candidate proteins. <i>Results:</i>
The proteomic analysis of the IPMN Liquid Tissue lysate resulted in
identification of 1,534 peptides corresponding to 523 unique proteins. A
subset of 25 proteins was identified that had previously been reported
as upregulated in pancreatic cancer. Immunohistochemical analysis for
two of these, deleted in malignant brain tumors 1 (DMBT1) and tissue
transglutaminase 2 (TGM2), confirmed their overexpression in IPMNs. <i>Conclusion:</i>
Global proteomics analysis using the Liquid Tissue workflow is a
feasible approach for unbiased biomarker discovery in limited archival
material, particularly applicable to precursor lesions of cancer.</p